Human LRRC3B Antibody

Catalog # Availability Size / Price Qty
MAB7635
MAB7635-SP
Detection of Human LRRC3B by Western Blot.
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Human LRRC3B Antibody Summary

Species Reactivity
Human
Specificity
Detects human LRRC3B in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # 758315
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human LRRC3B
Phe28-Ala205
Accession # Q96PB8
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human LRRC3B antibody by Western Blot. View Larger

Detection of Human LRRC3B by Western Blot. Western blot shows lysates of human prostate tissue and human testis tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human LRRC3B Monoclonal Antibody (Catalog # MAB7635) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for LRRC3B at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LRRC3B

LRRC3B, also known as LRP15, is a transmembrane leucine-rich protein that is downregulated by promoter methylation in gastric and colorectal cancers. It confers resistance to ultraviolet light-induced DNA damage and functions as a tumor suppressor. Mature human LRRC3B contains an LRR N-terminal domain (aa 34-64), 3 LRR repeats (aa 65-135), an LRR C-terminal domain (aa 145-197), a transmembrane segment (aa 205-225), and a C-terminal region (aa 226-259). Within aa 28-205, human LRRC3B shares 100% aa sequence identity with mouse and rat LRRC3B.

Long Name
Leucine Rich Repeat Containing 3B
Entrez Gene IDs
116135 (Human); 218763 (Mouse)
Alternate Names
leucine rich repeat containing 3B; leucine-rich repeat-containing protein 3B; LRP15; LRP15Leucine-rich repeat protein LRP15; LRRC3B; MGC102927

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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