Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 758315
View all LRRC3B Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human LRRC3B
Phe28-Ala205
Accession # Q96PB8

Specificity

Detects human LRRC3B in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human LRRC3B Antibody

Detection of Human LRRC3B antibody by Western Blot.

Detection of Human LRRC3B by Western Blot.

Western blot shows lysates of human prostate tissue and human testis tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human LRRC3B Monoclonal Antibody (Catalog # MAB7635) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for LRRC3B at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human LRRC3B Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: human prostate tissue and human testis tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LRRC3B

LRRC3B, also known as LRP15, is a transmembrane leucine-rich protein that is downregulated by promoter methylation in gastric and colorectal cancers. It confers resistance to ultraviolet light-induced DNA damage and functions as a tumor suppressor. Mature human LRRC3B contains an LRR N-terminal domain (aa 34-64), 3 LRR repeats (aa 65-135), an LRR C-terminal domain (aa 145-197), a transmembrane segment (aa 205-225), and a C-terminal region (aa 226-259). Within aa 28-205, human LRRC3B shares 100% aa sequence identity with mouse and rat LRRC3B.

Long Name

Leucine Rich Repeat Containing 3B

Alternate Names

LRP15

Entrez Gene IDs

116135 (Human); 218763 (Mouse)

Gene Symbol

LRRC3B

UniProt

Q96PB8 (Human)

Additional LRRC3B Products

Product Documents for Human LRRC3B Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human LRRC3B Antibody

For research use only

Related Research Areas

Cancer Biomarkers

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