Detection of Human LRRC3B by Western Blot. Western blot shows lysates of human prostate tissue and human testis tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human LRRC3B Monoclonal Antibody (Catalog # MAB7635) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for LRRC3B at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
LRRC3B, also known as LRP15, is a transmembrane leucine-rich protein that is downregulated by promoter methylation in gastric and colorectal cancers. It confers resistance to ultraviolet light-induced DNA damage and functions as a tumor suppressor. Mature human LRRC3B contains an LRR N-terminal domain (aa 34-64), 3 LRR repeats (aa 65-135), an LRR C-terminal domain (aa 145-197), a transmembrane segment (aa 205-225), and a C-terminal region (aa 226-259). Within aa 28-205, human LRRC3B shares 100% aa sequence identity with mouse and rat LRRC3B.
Leucine Rich Repeat Containing 3B
Entrez Gene IDs:
116135 (Human); 218763 (Mouse)
leucine rich repeat containing 3B; leucine-rich repeat-containing protein 3B; LRP15; LRP15Leucine-rich repeat protein LRP15; MGC102927
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