Human Lymphotoxin Antibody

Catalog #: MAB1370
2 Citations Datasheet / COA / SDS

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MAB1370
MAB1370-SP

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Cytotoxicity Induced by Lymphotoxin alpha 2/ beta 1 and Neutralization by Human Lymphotoxin Antibody.

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Citations (2)

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Human Lymphotoxin Antibody Summary

Species Reactivity

Human

Specificity

Recognizes human Lymphotoxin alpha chains in alpha ₂ beta ₁ or alpha ₁ beta ₂ trimers and also human TNF-beta. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) APRIL, rhBAFF, rhEDA-A2, rhFas Ligand, rhLIGHT, rhGITR Ligand, rhOX40 Ligand, recombinant mouse RANKL, rhTNF-alpha, rhTRAIL, rhTRANCE, rhTWEAK, or rhVEGI is observed.

Source

Monoclonal Mouse IgG₁ Clone # 135125

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Lymphotoxin alpha / beta

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration

Sample

Neutralization

Measured by its ability to neutralize Lymphotoxin alpha 2/ beta 1-induced cytotoxicity in the L‑929 mouse fibroblast cell line. Matthews, N. and M. L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M. J. et al. (eds): IRL Press. 221. The Neutralization Dose (ND₅₀) is typically 0.15-0.9 µg/mL in the presence of 10 ng/mL Recombinant Human Lymphotoxin alpha 2/ beta 1 and 1 µg/mL actinomycin D.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Cytotoxicity Induced by Lymphotoxin alpha 2/ beta 1 and Neutralization by Human Lymphotoxin Antibody. Recombinant Human Lympho-toxin a₂/ beta ₁(Catalog # 679-TX) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin. Cytotoxicity elicited by Recombinant Human Lymphotoxin a₂/ beta ₁is neutralized (green line) by increasing concen-trations of Mouse Anti-Human Lymphotoxin Monoclonal Antibody (Catalog # MAB1370). The ND₅₀ is typically 0.15-0.9 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lymphotoxin

Lymphotoxin alpha, also known as tumor necrosis factor (TNF) beta, and Lymphotoxin beta are members of the TNF superfamily and have been designated TNFSF1 and TNFSF3, respectively. Secreted Lymphotoxin alpha forms a homotrimer (TNF-beta) and heterotrimeric complexes with the type II membrane protein, Lymphotoxin beta (either alpha ₂ beta ₁ or alpha ₁ beta ₂ timers). These trimers serve as ligands for Lymphotoxin beta receptor, TNF RI and TNF RII and play a role in the development and maturation of the immune system.

Entrez Gene IDs

4049 (Human); 16992 (Mouse); 102138497 (Cynomolgus Monkey)

Alternate Names

LT; Lymphotoxin alpha; Lymphotoxin; tnfb; TNFSF1; TNLG1E

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human Lymphotoxin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Innate lymphoid cells integrate stromal and immunological signals to enhance antibody production by splenic marginal zone B cells.
Authors: Magri G, Miyajima M, Bascones S, Mortha A, Puga I, Cassis L, Barra C, Comerma L, Chudnovskiy A, Gentile M, Llige D, Cols M, Serrano S, Arostegui J, Juan M, Yague J, Merad M, Fagarasan S, Cerutti A
Nat Immunol, 2014;15(4):354-64.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
A role for lymphotoxin in primary Sjogren's disease.
Authors: Shen L, Suresh L, Wu J, Xuan J, Li H, Zhang C, Pankewycz O, Ambrus JL
J. Immunol., 2010;185(10):6355-63.
Species: Human
Sample Types: Saliva
Applications: Western Blot