Human Lymphotoxin Antibody

Catalog # Availability Size / Price Qty
MAB1370
MAB1370-SP
Cytotoxicity Induced by Lymphotoxin  alpha 2/ beta 1 and Neutralization by Human Lymphotoxin Antibody.
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Citations (2)
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Human Lymphotoxin Antibody Summary

Species Reactivity
Human
Specificity
Recognizes human Lymphotoxin alpha chains in alpha 2 beta 1 or alpha 1 beta 2 trimers and also human TNF-beta. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) APRIL, rhBAFF, rhEDA-A2, rhFas Ligand, rhLIGHT, rhGITR Ligand, rhOX40 Ligand, recombinant mouse RANKL, rhTNF-alpha, rhTRAIL, rhTRANCE, rhTWEAK, or rhVEGI is observed.
Source
Monoclonal Mouse IgG1 Clone # 135125
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Lymphotoxin  alpha / beta
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Neutralization
Measured by its ability to neutralize Lymphotoxin alpha 2/ beta 1-induced cytotoxicity in the L‑929 mouse fibroblast cell line. Matthews, N. and M. L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M. J. et al. (eds): IRL Press. 221. The Neutralization Dose (ND50) is typically 0.15-0.9 µg/mL in the presence of 10 ng/mL Recombinant Human Lymphotoxin alpha 2/ beta 1 and 1 µg/mL actinomycin D.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization Cytotoxicity Induced by Lymphotoxin  alpha 2/ beta 1 and Neutralization by Human Lymphotoxin Antibody. View Larger

Cytotoxicity Induced by Lymphotoxin  alpha 2/ beta 1 and Neutralization by Human Lymphotoxin Antibody.

Recombinant Human Lympho­toxin  alpha 2/ beta 1 (Catalog # 679-TX) induces cytotoxicity in the the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin. Cytotoxicity elicited by Recombinant Human Lymphotoxin  alpha 2/ beta 1 is neutralized (green line) by increasing concen­trations of Mouse Anti-Human Lymphotoxin Monoclonal Antibody (Catalog # MAB1370). The ND50 is typically 0.15‑0.9 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lymphotoxin

Lymphotoxin alpha, also known as tumor necrosis factor (TNF) beta, and Lymphotoxin beta are members of the TNF superfamily and have been designated TNFSF1 and TNFSF3, respectively. Secreted Lymphotoxin alpha forms a homotrimer (TNF-beta) and heterotrimeric complexes with the type II membrane protein, Lymphotoxin beta (either alpha 2 beta 1 or alpha 1 beta 2 timers). These trimers serve as ligands for Lymphotoxin beta receptor, TNF RI and TNF RII and play a role in the development and maturation of the immune system.

Entrez Gene IDs
4049 (Human); 16992 (Mouse)
Alternate Names
LT; Lymphotoxin alpha; Lymphotoxin; tnfb; TNFSF1; TNLG1E

Product Datasheets

Citations for Human Lymphotoxin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Innate lymphoid cells integrate stromal and immunological signals to enhance antibody production by splenic marginal zone B cells.
    Authors: Magri G, Miyajima M, Bascones S, Mortha A, Puga I, Cassis L, Barra C, Comerma L, Chudnovskiy A, Gentile M, Llige D, Cols M, Serrano S, Arostegui J, Juan M, Yague J, Merad M, Fagarasan S, Cerutti A
    Nat Immunol, 2014;15(4):354-64.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  2. A role for lymphotoxin in primary Sjogren's disease.
    Authors: Shen L, Suresh L, Wu J, Xuan J, Li H, Zhang C, Pankewycz O, Ambrus JL
    J. Immunol., 2010;185(10):6355-63.
    Species: Human
    Sample Types: Saliva
    Applications: WB

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Isotype Controls

Mouse IgG1 Isotype Control

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Reconstitution Buffers

Secondary Antibodies

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