Human Lymphotoxin Antibody

Catalog # Availability Size / Price Qty
Cytotoxicity Induced by Lymphotoxin  alpha 2/ beta 1 and Neutralization by Human Lymphotoxin Antibody.
1 Image
Product Details
Citations (2)
Supplemental Products

Human Lymphotoxin Antibody Summary

Species Reactivity
Recognizes human Lymphotoxin alpha chains in alpha 2 beta 1 or alpha 1 beta 2 trimers and also human TNF-beta. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) APRIL, rhBAFF, rhEDA-A2, rhFas Ligand, rhLIGHT, rhGITR Ligand, rhOX40 Ligand, recombinant mouse RANKL, rhTNF-alpha, rhTRAIL, rhTRANCE, rhTWEAK, or rhVEGI is observed.
Monoclonal Mouse IgG1 Clone # 135125
Protein A or G purified from hybridoma culture supernatant
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Lymphotoxin  alpha / beta
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.


Recommended Concentration
Measured by its ability to neutralize Lymphotoxin alpha 2/ beta 1-induced cytotoxicity in the L‑929 mouse fibroblast cell line. Matthews, N. and M. L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M. J. et al. (eds): IRL Press. 221. The Neutralization Dose (ND50) is typically 0.15-0.9 µg/mL in the presence of 10 ng/mL Recombinant Human Lymphotoxin alpha 2/ beta 1 and 1 µg/mL actinomycin D.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cytotoxicity Induced by Lymphotoxin  alpha 2/ beta 1 and Neutralization by Human Lymphotoxin Antibody. View Larger

Cytotoxicity Induced by Lymphotoxin alpha 2/ beta 1 and Neutralization by Human Lymphotoxin Antibody. Recombinant Human Lympho-toxin a2/ beta 1(Catalog # 679-TX) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin. Cytotoxicity elicited by Recombinant Human Lymphotoxin a2/ beta 1is neutralized (green line) by increasing concen-trations of Mouse Anti-Human Lymphotoxin Monoclonal Antibody (Catalog # MAB1370). The ND50 is typically 0.15-0.9 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lymphotoxin

Lymphotoxin alpha, also known as tumor necrosis factor (TNF) beta, and Lymphotoxin beta are members of the TNF superfamily and have been designated TNFSF1 and TNFSF3, respectively. Secreted Lymphotoxin alpha forms a homotrimer (TNF-beta) and heterotrimeric complexes with the type II membrane protein, Lymphotoxin beta (either alpha 2 beta 1 or alpha 1 beta 2 timers). These trimers serve as ligands for Lymphotoxin beta receptor, TNF RI and TNF RII and play a role in the development and maturation of the immune system.

Entrez Gene IDs
4049 (Human); 16992 (Mouse); 102138497 (Cynomolgus Monkey)
Alternate Names
LT; Lymphotoxin alpha; Lymphotoxin; tnfb; TNFSF1; TNLG1E

Product Datasheets

You must select a language.


Citations for Human Lymphotoxin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

  1. Innate lymphoid cells integrate stromal and immunological signals to enhance antibody production by splenic marginal zone B cells.
    Authors: Magri G, Miyajima M, Bascones S, Mortha A, Puga I, Cassis L, Barra C, Comerma L, Chudnovskiy A, Gentile M, Llige D, Cols M, Serrano S, Arostegui J, Juan M, Yague J, Merad M, Fagarasan S, Cerutti A
    Nat Immunol, 2014;15(4):354-64.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  2. A role for lymphotoxin in primary Sjogren's disease.
    Authors: Shen L, Suresh L, Wu J, Xuan J, Li H, Zhang C, Pankewycz O, Ambrus JL
    J. Immunol., 2010;185(10):6355-63.
    Species: Human
    Sample Types: Saliva
    Applications: Western Blot


No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Lymphotoxin Antibody

There are currently no reviews for this product. Be the first to review Human Lymphotoxin Antibody and earn rewards!

Have you used Human Lymphotoxin Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review