Human Lymphotoxin  beta R/TNFRSF3 Antibody MAB6291-100: R&D Systems

Human Lymphotoxin  beta R/TNFRSF3 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Lymphotoxin beta R/TNFRSF3 in direct ELISAs.
  • Source
    Monoclonal Mouse IgG1 Clone # 71315
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Lymphotoxin beta R/TNFRSF3
    Gln31-Met227
    Accession # P36941
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Agonist Activity
    Measured by its ability to induce CXCL8/IL-8 secretion in the A375 human melanoma cell line. The Neutralization Dose (ND50) is typically 1.5-9 ng/mL.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
CXCL8/IL-8 Secretion Induced by Human Lymphotoxin beta R/TNFRSF3 Antibody. Human Lymphotoxin beta R/TNFRSF3 Monoclonal Antibody (Catalog # MAB6291) activates Lymphotoxin beta Receptor on the A375 human melanoma cell line in a dose-dependent manner which inducesCXCL8/IL-8 Secretion, as measured by the Human CXCL8/IL-8 Quantikine ELISA (Catalog # D8000C). The ND50is typically 1.5-9 ng/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Lymphotoxin beta R/TNFRSF3

Lymphotoxin beta receptor (LT beta R), also known as TNF RIII and TNF R-related protein (TNF Rrp) is a member of the TNF receptor superfamily, designated TNFRSF3. Human LT beta R cDNA encodes a 435 amino acid (aa) residue type I membrane protein with a putative 30 aa residue signal peptide, a 193 aa residue extracellular domain and a 171 aa residue cytoplasmic domain. The extracellular domain of LT beta R contains four cysteine-rich motifs characteristic of the TNF receptor superfamily. The cytoplasmic region of LT beta R shares little sequence similarity with other TNF receptor family members, suggesting that different signaling mechanisms may be used. LT beta R is expressed in a variety of tissues including visceral and lymphoid tissues. LT beta R is also expressed by cell lines of monocytic, epithelial, and fibroblastic origins but not by T and B lymphocytes. Human and mouse LT beta R share 76% aa sequence homology. The TNF family ligands that have been shown to bind and activate LT beta R include LIGHT (also a ligand for HVEM) and the heterotrimeric lymphotoxin LT alpha 1/ beta 2 or LT alpha 2/ beta 1. Depending on the cell type, activation of LT beta R has been shown to induce NF kappa B activation, chemokine production, growth arrest, and apoptosis. In vivo, LT beta R has been shown to play a critical role in controlling cellular immune functions and lymphoid organogenesis.

  • References:
    1. Zhai, Y. et al. (1998) J. Clin. Invest. 102:1142.
    2. Rennert, P.D. et al. (1998) Immunity 9:71.
    3. Degli-Esposti, M.A. et al. (1997) J. Immunol 158:1756.
    4. Mackay, F. et al. (1996) J. Biol. Chem. 271:8618.
    5. Crowe, P.D. et al. (1994) Science 264:707.
  • Long Name:
    Lymphotoxin beta Receptor
  • Entrez Gene IDs:
    4055 (Human); 17000 (Mouse); 297604 (Rat)
  • Alternate Names:
    CD18; D12S370; ltbetar; LT-BETA-R; LTBR; lymphotoxin B receptor; Lymphotoxin beta R; lymphotoxin beta receptor (TNFR superfamily, member 3); Lymphotoxin-beta receptor; LymphotoxinbR; TNF RIII; TNF Rrp; TNFCRTNF-RIII; TNFR superfamily, member 3; TNFR2-RP; TNFR3; TNF-R-III; TNFR-RP; TNFRSF3; TNFRSF3TNFR-III; Tumor necrosis factor C receptor; Tumor necrosis factor receptor 2-related protein; tumor necrosis factor receptor superfamily member 3; tumor necrosis factor receptor superfamily, member 3; Tumor necrosis factor receptor type III
Related Research Areas
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

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Mouse IgG Horseradish Peroxidase-conjugated Antibody

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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse IgG Antibody

WB AF007 3
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 3  
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 2
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 1
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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