Human Lymphotoxin  beta R/TNFRSF3 Antibody

Catalog # Availability Size / Price Qty
MAB6291-100
MAB6291-SP
CXCL8/IL-8 Secretion Induced by Human Lymphotoxin  beta R/TNFRSF3 Antibody.
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Human Lymphotoxin  beta R/TNFRSF3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Lymphotoxin beta R/TNFRSF3 in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 71315
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Lymphotoxin beta R/TNFRSF3
Gln31-Met227
Accession # P36941
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Agonist Activity
Measured by its ability to induce CXCL8/IL-8 secretion in the A375 human melanoma cell line. The Neutralization Dose (ND50) is typically 1.5-9 ng/mL.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Agonist Activity CXCL8/IL-8 Secretion Induced by Human Lymphotoxin  beta R/TNFRSF3 Antibody. View Larger

CXCL8/IL-8 Secretion Induced by Human Lymphotoxin beta R/TNFRSF3 Antibody. Human Lymphotoxin beta R/TNFRSF3 Monoclonal Antibody (Catalog # MAB6291) activates Lymphotoxin beta Receptor on the A375 human melanoma cell line in a dose-dependent manner which inducesCXCL8/IL-8 Secretion, as measured by the Human CXCL8/IL-8 Quantikine ELISA (Catalog # D8000C). The ND50is typically 1.5-9 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lymphotoxin beta R/TNFRSF3

Lymphotoxin beta receptor (LT beta R), also known as TNF RIII and TNF R-related protein (TNF Rrp) is a member of the TNF receptor superfamily, designated TNFRSF3. Human LT beta R cDNA encodes a 435 amino acid (aa) residue type I membrane protein with a putative 30 aa residue signal peptide, a 193 aa residue extracellular domain and a 171 aa residue cytoplasmic domain. The extracellular domain of LT beta R contains four cysteine-rich motifs characteristic of the TNF receptor superfamily. The cytoplasmic region of LT beta R shares little sequence similarity with other TNF receptor family members, suggesting that different signaling mechanisms may be used. LT beta R is expressed in a variety of tissues including visceral and lymphoid tissues. LT beta R is also expressed by cell lines of monocytic, epithelial, and fibroblastic origins but not by T and B lymphocytes. Human and mouse LT beta R share 76% aa sequence homology. The TNF family ligands that have been shown to bind and activate LT beta R include LIGHT (also a ligand for HVEM) and the heterotrimeric lymphotoxin LT alpha 1/ beta 2 or LT alpha 2/ beta 1. Depending on the cell type, activation of LT beta R has been shown to induce NF kappa B activation, chemokine production, growth arrest, and apoptosis. In vivo, LT beta R has been shown to play a critical role in controlling cellular immune functions and lymphoid organogenesis.

References
  1. Zhai, Y. et al. (1998) J. Clin. Invest. 102:1142.
  2. Rennert, P.D. et al. (1998) Immunity 9:71.
  3. Degli-Esposti, M.A. et al. (1997) J. Immunol 158:1756.
  4. Mackay, F. et al. (1996) J. Biol. Chem. 271:8618.
  5. Crowe, P.D. et al. (1994) Science 264:707.
Long Name
Lymphotoxin beta Receptor
Entrez Gene IDs
4055 (Human); 17000 (Mouse); 297604 (Rat)
Alternate Names
CD18; D12S370; ltbetar; LT-BETA-R; LTBR; lymphotoxin B receptor; Lymphotoxin beta R; lymphotoxin beta receptor (TNFR superfamily, member 3); Lymphotoxin-beta receptor; LymphotoxinbR; TNF RIII; TNF Rrp; TNFCRTNF-RIII; TNFR superfamily, member 3; TNFR2-RP; TNFR3; TNF-R-III; TNFR-RP; TNFRSF3; TNFRSF3TNFR-III; Tumor necrosis factor C receptor; Tumor necrosis factor receptor 2-related protein; tumor necrosis factor receptor superfamily member 3; tumor necrosis factor receptor superfamily, member 3; Tumor necrosis factor receptor type III

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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