Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 961703
View all LYPD8 Primary Antibodies »

Product Specifications

Immunogen

Human embryonic kidney cell line HEK293-derived transfected with human LYPD8
Met1-Asn215
Accession # Q6UX82

Specificity

Detects human LYPD8 in direct ELISAs. Stains human LYPD8 transfectants but not irrelevant transfectants in flow cytometry.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human LYPD8 Antibody

Detection of LYPD8 antibody in HEK293 Human Cell Line Transfected with Human LYPD8 and eGFP antibody by Flow Cytometry.

Detection of LYPD8 in HEK293 Human Cell Line Transfected with Human LYPD8 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with either (A) human LYPD8 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human LYPD8 Monoclonal Antibody (Catalog # MAB9087) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.
LYPD8 antibody in HEK293 Human Cell Line by Immunocytochemistry (ICC).

LYPD8 in HEK293 Human Cell Line.

LYPD8 was detected in immersion fixed HEK293 human embryonic kidney cell line using Mouse Anti-Human LYPD8 Monoclonal Antibody (Catalog # MAB9087) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell membrane and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human LYPD8 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with human LYPD8 and eGFP

Immunocytochemistry

5-25 µg/mL
Sample: Immersion fixed HEK293 human embryonic kidney cell line

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LYPD8

Ly6/PLAUR domain containing 8 (LYPD8), is a GPI-linked protein with structural similarity to the urokinase-type plasminogen activator receptor (uPAR) (1). Mature human LYPD8 contains one uPAR/Ly6 domain and a Ser/Thr/Pro-rich (STP) region that may serve as a target for protease mediated shedding as has been shown for the related C4.4A/LYPD3 molecule (2, 3). Mature human LYPD8 shares 40% amino acid sequence identity with mouse and rat LYPD8.

References

  1. Kong, H.K. and J.H. Park (2012) BMB Rep. 45:595.
  2. Hansen, L.V. et al. (2004) Biochem. J. 380:845.
  3. Esselens, C.W. et al. (2008) Biol. Chem. 389:1075.

Long Name

Ly6/PLAUR Domain-containing Protein 8

Entrez Gene IDs

646627 (Human); 70163 (Mouse); 691259 (Rat)

Gene Symbol

LYPD8

UniProt

Q6UX82

Additional LYPD8 Products

Product Documents for Human LYPD8 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human LYPD8 Antibody

For research use only

Related Research Areas

Cancer Biomarkers

Citations for Human LYPD8 Antibody

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Protocols

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