|Detection of Human MAD1L1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human MAD1L1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4194) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for MAD1L1 at approximately 90 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.|
MAD1L1 (mitotic arrest deficient 1-like 1) is a member of the spindle-assembly checkpoint (SAC) which prevents anaphase from occurring until all chromosomes are properly aligned at the metaphase plate. Upon checkpoint activation, MAD1L1 recruits MAD2 to unattached kinetochores, promoting the binding of MAD2 to CDC20, the activator for the anaphase-promoting complex. MAD1L1 has been implicated to have tumor-suppressing functions, but in small-cell lung cancer cell lines, the MAD1L1 gene is the most frequent copy number gain, suggesting a more complex role.
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