Human Max Antibody Summary
Accession # P61244
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human Max by Western Blot. Western blot shows lysates of LNCaP human prostate cancer cell line and PC-3 human prostate cancer cell line. Gels were loaded with 20 µg of cytoplasmic (Cyto) and 10 µg of nuclear extracts (Nuc). PVDF membrane was probed with 1 µg/mL Goat Anti-Human Max Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4304) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for Max was detected at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Max is a member of the basic helix-loop-helix leucine zipper (bHLHZ) family of transcription factors. Max is able to form homodimers and heterodimers with other family members (Mad, Mxi1, and Myc). These homodimers and heterodimers compete for a common DNA target site (the E box (CACGTG)). MYC:MAX heterodimers are associated with transcriptional activation and cellular proliferation, whereas, the MAD:MAX heterodimers are associated with transcriptional repression and cellular differentiation. Max has six alternatively spliced transcript variants.
Citation for Human Max Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Reversible linkage of two distinct small molecule inhibitors of Myc generates a dimeric inhibitor with improved potency that is active in myc over-expressing cancer cell lines.
Authors: Wanner, Jutta, Romashko, Darlene, Werner, Douglas, May, Earl W, Peng, Yue, Schulz, Ryan, Foreman, Kenneth, Russo, Suzanne, Arnold, Lee D, Pingle, Maneesh, Bergstrom, Donald E, Barany, Francis, Thomson, Stuart
PLoS ONE, 2015;10(4):e0121793.
Sample Types: Cell Lysates
Applications: Western Blot
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