Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Methionine Aminopeptidase 1D/MAP1D Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Methionine Aminopeptidase 1D/MAP1D
Arg44-Ala335
Accession # Q6UB28

Specificity

Detects human Methionine Aminopeptidase 1D/MAP1D in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human Methionine Aminopeptidase 1D/MAP1D Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Methionine Aminopeptidase 1D/MAP1D (Catalog # 4714-ZN), see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Human Methionine Aminopeptidase 1D/MAP1D (Catalog # 4714-ZN)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Methionine Aminopeptidase 1D/MAP1D

Methionine Aminopeptidase 1D (MAP1D) is a member of the Methionine Aminopeptidase family (MetAPs) (1). MetAPs catalyze the removal of the initiator methionine residue from nascent peptides (2) and are essential for cell growth (3). MAP1D is localized in mitochondria (4) and is over-expressed in colon cancer cell lines and colon tumors (1). The N-terminal leader peptide (residue 1‑43) of MAP1D has been shown to be responsible for targeting to mitochondria (4).

References

  1. Leszczyniecka, M. et al. (2006) Oncogene 25:3471.
  2. Lowther, W.T. and B.W. Matthews (2000) Biochim. Biophys. Acta. 1477:157.
  3. Li, X. and Y.H. Chang (1995) Proc. Natl. Acad. Sci. USA 92:12357.
  4. Serero, A. et al. (2003) J. Biol. Chem. 278:52953.

Alternate Names

MAP1D, MetAP1D

Entrez Gene IDs

254042 (Human)

Gene Symbol

METAP1D

UniProt

Q6UB28

Additional Methionine Aminopeptidase 1D/MAP1D Products

Product Documents for Human Methionine Aminopeptidase 1D/MAP1D Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Methionine Aminopeptidase 1D/MAP1D Antibody

For research use only

Related Research Areas

Metalloproteases and Regulators

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