Key Product Details

Species Reactivity

Human

Applications

Western Blot, Intracellular Staining by Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 464818
View all mGluR5 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human mGluR5
Gln21-Ser509
Accession # P41594

Specificity

Detects human mGluR5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human mGluR1, R2, R3, R4, R7, or R8 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human mGluR5 Antibody

Detection of mGluR5 antibody in MG‑63 Human Cell Line antibody by Flow Cytometry.

Detection of mGluR5 in MG‑63 Human Cell Line by Flow Cytometry.

MG‑63 human osteosarcoma cell line was stained with Mouse Anti-Human mGluR5 Monoclonal Antibody (Catalog # MAB4514, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Applications for Human mGluR5 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/106 cells
Sample: MG‑63 human osteosarcoma cell line fixed with paraformaldehyde and permeabilized with saponin

Western Blot

1 µg/mL
Sample: Recombinant Human mGluR5

Reviewed Applications

Read 1 review rated 4 using MAB4514 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: mGluR5

Human metabotropic glutamate receptor 5 (mGluR5; also mGluR5b) is a 150 kDa 7-transmembrane glycoprotein that belongs to group I of the C-family of G-protein coupled receptors. mGluR5 is constitutively expressed and regulates neuronal ion channel activity. Human mGluR5 is 1212 aa in length and contains an extracellular domain (ECD) of 558 amino acids. Through its ECD, mGluR5 either homodimerizes or heterodimerizes with the Ca++-sensor receptor. There is one alternate splice form (mGluR5a) that shows a 32 aa deletion between aa 877‑908 in the cytoplasmic tail. Over aa 19‑509, human mGluR5 is 98% aa identical to mouse, rat, and dog mGluR5.

Long Name

Metabotropic Glutamate Receptor 5

Alternate Names

GPRC1E, GRM5, mGlu5

Entrez Gene IDs

2915 (Human); 108071 (Mouse); 24418 (Rat)

Gene Symbol

GRM5

UniProt

P41594

Additional mGluR5 Products

Product Documents for Human mGluR5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human mGluR5 Antibody

For research use only

Citations for Human mGluR5 Antibody

Customer Reviews for Human mGluR5 Antibody (1)

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  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: See PMID 24236200
    Species: Human
    Verified Customer | Posted 02/20/2015

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