Human MIF Antibody

  (14 citations)
(1 Review)
    
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Product Details
Citations (14)
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  • Species Reactivity
    Human
  • Specificity
    Detects human MIF in Western blots. In Western blots, this antibody shows approximately 25% cross-reactivity with recombinant mouse MIF.
  • Source
    Monoclonal Mouse IgG1 Clone # 12302
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human MIF
    Pro2-Ala115
    Accession # P14174
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Simple Western
    10 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human MIF by Western Blot. Western blot shows lysate of K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human MIF Monoclonal Antibody (Catalog # MAB289) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MIF at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Humean MIF by Simple WesternTM. Simple Western lane view shows lysates of THP‑1 human acute monocytic leukemia cell line and U937 human histiocytic lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for MIF at approximately 12 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human MIF Monoclonal Antibody (Catalog # MAB289). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.        
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MIF

MIF (or macrophage migration inhibitory factor) was the first lymphokine/cytokine to be recognized in the pregenomics era (1, 2). Regardless, it is one of the least understood of all inflammatory mediators (1, 3). Human MIF is a 12.5 kDa, 115 amino acid (aa) nonglycosylated polypeptide that is synthesized without a signal sequence (4-7). Secretion occurs nonclassically via an ABCA1 transporter (8). The initiating Met is removed, leaving Pro as the first amino acid. The molecule consists of two alpha -helices and six beta -strands, four of which form a beta -sheet. The two remaining beta -strands interact with other MIF molecules, creating a trimer (2, 9, 10). Structure-function studies suggest MIF is bifunctional with segregated topology. The N- and C-termini mediate enzyme activity (in theory). Phenylpyruvate tautomerase activity (enol-to-keto) has been demonstrated and is dependent upon Pro at position #1 (11). Amino acids 50-65 have also been suggested to contain thiol-protein oxidoreductase activity (12). MIF has proinflammatory cytokine activity centered around aa’s 49-65. On fibroblasts, MIF induces, IL-1, IL-8, and MMP expression; on macrophages, MIF stimulates NO production and TNF-alpha release following IFN-gamma activation (13, 14). MIF apparently acts through CD74 and CD44, likely in some form of trimeric interaction (15, 16). Human MIF is active on mouse cells (14). Human MIF is 90%, 94%, 95%, and 90% aa identical to mouse, bovine, porcine, and rat MIF, respectively.

  • References:
    1. Norand, E.F. and M. Leech (2005) Front. Biosci. 10:12.
    2. Donn, R.P. and D.W. Ray (2004) J. Endocrinol. 182:1.
    3. Calandra, T. and T. Roger (2003) Nat. Rev. Immunol. 3:791.
    4. Kozak, C.A. et al. (1995) Genomics 27:405.
    5. Weiser, W.Y. et al. (1989) Proc. Natl. Acad. Sci. USA 86:7522.
    6. Paralkar, V. and G. Wistow (1994) Genomics 19:48.
    7. Wistow, G.J. et al. (1993) Proc. Natl. Acad. Sci. USA 90:1272.
    8. Flieger, O. et al. (2003) FEBS Lett. 551:78.
    9. Philo, J.S. et al. (2004) Biophys. Chem. 108:77.
    10. Sun, H-W. et al. (1996) Protein Eng. 9:631.
    11. Stamps, S.L. et. al. (2000) Biochemistry 39:9671.
    12. Nguyen, M.T. et al. (2003) J. Biol. Chem. 278:33654.
    13. Sato, A. et al. (2003) Dev. Comp. Immunol. 27:401.
    14. Bernhagen, J. et al. (1994) Biochemistry 33:14144.
    15. Leng, L. et al. (2003) J. Exp. Med. 197:1467.
    16. Meyer-Siegler, K.L. and P.L. Vera (2005) J. Urol. 173:615.
  • Long Name:
    Macrophage Migration Inhibitory Factor
  • Entrez Gene IDs:
    4282 (Human); 17319 (Mouse)
  • Alternate Names:
    EC 5.3.2.1; EC 5.3.3.12; GIFmacrophage migration inhibitory factor; GLIF; Glycosylation-inhibiting factor; L-dopachrome isomerase; L-dopachrome tautomerase; macrophage migration inhibitory factor (glycosylation-inhibiting factor); MIF; MMIF; Phenylpyruvate tautomerase
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Association of Macrophage Migration Inhibitory Factor Polymorphisms with Total Plasma IgE Levels in Patients with Atopic Dermatitis in Korea
    Authors: JS Kim, J Choi, HJ Hahn, YB Lee, DS Yu, JW Kim
    PLoS ONE, 2016;11(9):e0162477.
    Species: Human
    Sample Type: Plasma
    Application: ELISA - Capture
  2. Macrophage Migration Inhibitory Factor in Acute Adipose Tissue Inflammation.
    Authors: Kim B, Rongisch R, Hager S, Grieb G, Nourbakhsh M, Rennekampff H, Bucala R, Bernhagen J, Pallua N
    PLoS ONE, 2015;10(9):e0137366.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: ELISA Capture
  3. iTRAQ Quantitative Proteomic Comparison of Metastatic and Non-Metastatic Uveal Melanoma Tumors.
    Authors: Crabb J, Hu B, Crabb J, Triozzi P, Saunthararajah Y, Tubbs R, Singh A
    PLoS ONE, 2015;10(8):e0135543.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: WB
  4. Macrophage migration inhibitory factor engages PI3K/Akt signalling and is a prognostic factor in metastatic melanoma.
    Authors: Oliveira, Camila S, de Bock, Charles, Molloy, Timothy, Sadeqzadeh, Elham, Geng, Xin Yan, Hersey, Peter, Zhang, Xu Dong, Thorne, Rick F
    BMC Cancer, 2014;14(0):630.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  5. A multiplex immunoassay for human adipokine profiling.
    Authors: Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E
    Clin. Chem., 2010;56(0):1320.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: Luminex Development
  6. Intracellular interaction of myosin light chain kinase with macrophage migration inhibition factor (MIF) in endothelium.
    Authors: Wadgaonkar R, Dudek SM, Zaiman AL, Linz-McGillem L, Verin AD, Nurmukhambetova S, Romer LH, Garcia JG
    J. Cell. Biochem., 2005;95(4):849-58.
    Species: Bovine
    Sample Type: Cell Lysates
    Application: IP
  7. Macrophage migration inhibitory factor stimulates angiogenic factor expression and correlates with differentiation and lymph node status in patients with esophageal squamous cell carcinoma.
    Authors: Ren Y, Law S, Huang X, Lee PY, Bacher M, Srivastava G, Wong J
    Ann. Surg., 2005;242(1):55-63.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  8. Elevated macrophage migration inhibitory factor (MIF) levels in the urine of patients with focal glomerular sclerosis.
    Authors: Matsumoto K, Maruyama N, Maruyama T, Ohnishi Y, Nonaka S, Inoshita A, Ito K, Kitajima S, Abe M, Satomura A, Fujita T
    Clin. Exp. Immunol., 2005;139(2):338-47.
    Species: Human
    Sample Type: Urine
    Application: ELISA Development
  9. Up-regulation of macrophage migration inhibitory factor in infants with acute neonatal necrotizing enterocolitis.
    Authors: Ren Y, Lin CL, Li Z, Chen XY, Huang X, Lui V, Nicholls J, Lan HY, Tam PK
    Histopathology, 2005;46(6):659-67.
    Species: Human
    Sample Type: Serum
    Application: Neut
  10. Regulatory role of C5a on macrophage migration inhibitory factor release from neutrophils.
    Authors: Riedemann NC, Guo RF, Gao H, Sun L, Hoesel M, Hollmann TJ, Wetsel RA, Zetoune FS, Ward PA
    J. Immunol., 2004;173(2):1355-9.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  11. High concentrations of circulating macrophage migration inhibitory factor in patients with severe blunt trauma: Is serum macrophage migration inhibitory factor concentration a valuable prognostic factor?
    Authors: Chuang CC, Hung CJ, Tsai MC, Chuang YC
    Crit. Care Med., 2004;32(3):734-9.
    Species: Human
    Sample Type: Serum
    Application: ELISA Development
  12. Estrogen modulates cutaneous wound healing by downregulating macrophage migration inhibitory factor.
    Authors: Ashcroft GS, Mills SJ, Lei K, Gibbons L, Jeong MJ, Taniguchi M, Burow M, Horan MA, Wahl SM, Nakayama T
    J. Clin. Invest., 2003;111(9):1309-18.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  13. Urinary levels of macrophage migration inhibitory factor in patients with IgA nephropathy.
    Authors: Matsumoto K, Kanmatsuse K
    Nephron, 2002;92(2):309-15.
    Species: Human
    Sample Type: Urine
    Application: ELISA Development
  14. Human V gamma 2V delta 2 T cells augment migration-inhibitory factor secretion and counteract the inhibitory effect of glucocorticoids on IL-1 beta and TNF-alpha production.
    Authors: Wang L, Das H, Kamath A, Li L, Bukowski JF
    J. Immunol., 2002;168(10):4889-96.
    Species: Human
    Sample Type: Cell Culture Supernates
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Isotype Controls
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Mouse IgG1 Isotype Control

Ctrl MAB002 148  
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Mouse IgG1 Isotype Control

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Reconstitution Buffers
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Reconstitution Buffer 1 (PBS)

RB01
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Secondary Antibodies
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Mouse IgG HRP-conjugated Antibody

WB HAF007 32  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 12  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow , IHC , ICC NL007 10
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow , IHC , ICC NL008 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 4  
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG Antibody

WB AF007 4
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow , IHC , ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

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Average Rating: 5 (Based on 1 review)

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ELISA Human MIF Antibody MAB289 Array MAB289
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 ELISA Human Anonymous 11/07/2017
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ELISA Human MIF Antibody MAB289
ELISA: Human MIF Antibody [MAB289].

Summary

Application ELISA
Sample Tested Serum and Plasma
Species Human

Other Experimental Details

Other Experimental Details This antibody was used as a matched pair with AF289 to build a sandwich ELISA to measure MIF in human serum and plasma. The antibody worked well.

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