Detection of Human MIF by Western Blot.
Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line and U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human MIF Monoclonal Antibody (Catalog # MAB2893) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MIF at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
MIF (or macrophage Migration Inhibitory Factor) was the first lymphokine/cytokine to be recognized in the pregenomics era (1, 2). Regardless, it is one of the least understood of all inflammatory mediators (1, 3). Human MIF is a 12.5 kDa, 115 amino acid (aa) nonglycosylated polypeptide that is synthesized without a signal sequence (4-7). Secretion occurs nonclassically via an ABCA1 transporter (8). The initiating Met is removed, leaving Pro as the first amino acid. The molecule consists of two alpha -helices and six beta -strands, four of which form a beta -sheet. The two remaining beta -strands interact with other MIF molecules, creating a trimer (2, 9, 10). Structure-function studies suggest MIF is bifunctional with segregated topology. The N- and C-termini mediate enzyme activity (in theory). Phenylpyruvate tautomerase activity (enol-to-keto) has been demonstrated and is dependent upon Pro at position #1 (11). Amino acids 50-65 have also been suggested to contain thiol-protein oxidoreductase activity (12). MIF has proinflammatory cytokine activity centered around aa’s 49-65. On fibroblasts, MIF induces, IL-1, IL-8, and MMP expression; on macrophages, MIF stimulates NO production and TNF-alpha release following IFN-gamma activation (13, 14). MIF apparently acts through CD74 and CD44, likely in some form of trimeric interaction (15, 16). Human MIF is active on mouse cells (14). Human MIF is 90%, 94%, 95%, and 90% aa identical to mouse, bovine, porcine, and rat MIF, respectively.
Norand, E.F. and M. Leech (2005) Front. Biosci. 10:12.
Donn, R.P. and D.W. Ray (2004) J. Endocrinol. 182:1.
Calandra, T. and T. Roger (2003) Nat. Rev. Immunol. 3:791.
Kozak, C.A. et al. (1995) Genomics 27:405.
Weiser, W.Y. et al. (1989) Proc. Natl. Acad. Sci. USA 86:7522.
Paralkar, V. and G. Wistow (1994) Genomics 19:48.
Wistow, G.J. et al. (1993) Proc. Natl. Acad. Sci. USA 90:1272.
Flieger, O. et al. (2003) FEBS Lett. 551:78.
Philo, J.S. et al. (2004) Biophys. Chem. 108:77.
Sun, H-W. et al. (1996) Protein Eng. 9:631.
Stamps, S.L. et. al. (2000) Biochemistry 39:9671.
Nguyen, M.T. et al. (2003) J. Biol. Chem. 278:33654.
Sato, A. et al. (2003) Dev. Comp. Immunol. 27:401.
Bernhagen, J. et al. (1994) Biochemistry 33:14144.
Leng, L. et al. (2003) J. Exp. Med. 197:1467.
Meyer-Siegler, K.L. and P.L. Vera (2005) J. Urol. 173:615.
Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.