Human Mindin Antibody

Catalog # Availability Size / Price Qty
AF2609
AF2609-SP
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews

Human Mindin Antibody Summary

Species Reactivity
Human
Specificity
Detects human Mindin in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Mindin
Glu32-Val331
Accession # Q9BUD6
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Mindin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Mindin

Mindin, also known as Spondin-2 and DIL-1 (Differentially expressed in cancerous and non-cancerous lung cells 1) belongs to the F-spondin family of secreted extracellular matrix proteins. Family members are characterized by two N-terminal F-spondin domains (FS1 and FS2), and a third C-terminal thrombospondin type I repeat (TSP-1). Secreted Mindin exists as a disulfide-linked dimer that also forms higher order oligomers. It functions as a pattern recognition receptor for microbes and an adhesion molecule for neurons. Mindin is secreted by neurons, macrophages and fibroblasts in a variety of tissues. Human Mindin shares 88% amino acid sequence homology with mouse and rat proteins.

Entrez Gene IDs
10417 (Human); 100689 (Mouse)
Alternate Names
Differentially expressed in cancerous and non-cancerous lung cells 1; DIL1; DIL-1; DIL1FLJ34460; DKFZp686G21139; FLJ16313; FLJ22401; Mindin; M-SPONDIN; RG-1; SPON2; Spondin 2; spondin 2, extracellular matrix protein; spondin-2

Product Datasheets

Citations for Human Mindin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. SPON2 Promotes M1-like Macrophage Recruitment and Inhibits Hepatocellular Carcinoma Metastasis by Distinct Integrin-Rho GTPase-Hippo Pathways
    Authors: YL Zhang, Q Li, XM Yang, F Fang, J Li, YH Wang, Q Yang, L Zhu, HZ Nie, XL Zhang, MX Feng, SH Jiang, GA Tian, LP Hu, HY Lee, SJ Lee, Q Xia, ZG Zhang
    Cancer Res., 2018;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  2. Spondin-2 (SPON2), a more prostate-cancer-specific diagnostic biomarker.
    Authors: Qian X, Li C, Pang B
    PLoS ONE, 2012;7(5):e37225.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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