Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry, Western Blot, ELISA Development

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Mindin Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Mindin
Glu32-Val331
Accession # Q9BUD6

Specificity

Detects human Mindin in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human Mindin Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human Mindin

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Mindin

Mindin, also known as Spondin-2 and DIL-1 (Differentially expressed in cancerous and non-cancerous lung cells 1) belongs to the F-spondin family of secreted extracellular matrix proteins. Family members are characterized by two N-terminal F-spondin domains (FS1 and FS2), and a third C-terminal thrombospondin type I repeat (TSP-1). Secreted Mindin exists as a disulfide-linked dimer that also forms higher order oligomers. It functions as a pattern recognition receptor for microbes and an adhesion molecule for neurons. Mindin is secreted by neurons, macrophages and fibroblasts in a variety of tissues. Human Mindin shares 88% amino acid sequence homology with mouse and rat proteins.

Alternate Names

DIL1, RG-1, SPON2, Spondin 2

Entrez Gene IDs

10417 (Human); 100689 (Mouse)

Gene Symbol

SPON2

UniProt

Q9BUD6

Additional Mindin Products

Product Documents for Human Mindin Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Mindin Antibody

For research use only

Citations for Human Mindin Antibody

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FAQs

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