Human/Mouse Aurora B Antibody Summary
Accession # Q96GD4
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human Aurora B by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line, and A20 mouse B cell lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human Aurora B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4006) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Aurora B at approximately 40 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Aurora B
Aurora B, a serine/threonine protein kinase, is a key regulator of the onset of cytokinesis and chromosome segregation. Phosphorylation of Histone H3 at Ser 10 and Ser 28 by Aurora B contributes to chromosome number instability and mitotic chromosome condensation. In addition, Aurora B phosphorylates MCAK (mitotic centromere-associated kinesin) on Ser 92 and this inhibits MCAK’s microtubule depolymerizing activity. Aurora B localizes to kinetochore fibers during chromosome movement and segregation.
Citation for Human/Mouse Aurora B Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Generation of human induced pluripotent stem cells using epigenetic regulators reveals a germ cell-like identity in partially reprogrammed colonies.
Authors: Goyal, Akshi, Chavez, Shawn L, Reijo Pera, Renee A
PLoS ONE, 2013;8(12):e82838.
Sample Types: Whole Cells
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