Human/Mouse BCAP Antibody

Detection of Human BCAP by Western Blot.

Western Blot shows lysates of RL human non-Hodgkin's lymphoma B cell line and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/ml of Goat Anti-Human/Mouse BCAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4857) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for BCAP at approximately 80 kDa and 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of Human BCAP/PIK3AP1 by Western Blot

BCAP is hyperphosphorylated in B-cell, macrophages, and Expi293F cells.A, lysates from THP-1 and Ramos cells were dephosphorylated with lambda -phosphatase and immunoblotted for BCAP. B, His-Avi-tagged BCAP expressed in Expi293F cells was purified and dephosphorylated with lambda -phosphatase before immunostaining for tyrosine and serine phosphorylation. C, phosphorylation sites of BCAP expressed in Expi293F cells were determined by phosphopeptide mapping. BCAP was digested with trypsin, chymotrypsin, Asp-N, and Glu-C prior to MS. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31527084), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human BCAP/PIK3AP1 by Western Blot

BCAP is phosphorylated by BTK, LYN, and SYK. Purified BCAP, dephosphorylated BCAP ( lambda -BCAP), and myelin basic protein (MyBP) were phosphorylated with (A) tyrosine kinases SYK, LYN, BTK, TYK2, and ITK, or (B) serine kinases CSNK1A1, CSNK2A2, and GSK-3. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31527084), licensed under a CC-BY license. Not internally tested by R&D Systems.