Detection of Human/Mouse BCAP by Western Blot. Western blot shows lysates of MO7e human megakaryocytic leukemic cell line and L1.2 mouse pro-B cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse BCAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4857) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for BCAP at approximately 100 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
BCAP (B-cell adaptor for phosphoinositide 3-kinase (PI3K)) participates in linking the B cell antigen receptor (BCR) with the PI3K pathway. Tyrosine phosphorylation of BCAP by BCR-associated protein tyrosine kinases, such as Syk and Btk, generates binding sites for the p85 subunit of PI3K, resulting in activation of the PI3K pathway. Human and mouse BCAP contain 3 YXXM SH2 binding motifs for interaction with PI3K.
B Cell Adaptor Protein
Entrez Gene IDs:
118788 (Human); 83490 (Mouse); 294048 (Rat)
1810044J04Rik; B cell adaptor protein; BCAPB-cell adapter for phosphoinositide 3-kinase; B-cell phosphoinositide 3-kinase adapter protein 1; FLJ35564; phosphoinositide 3-kinase adapter protein 1; phosphoinositide-3-kinase adaptor protein 1; Pik3ap1; RP11-34E5.3
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