Human/Mouse BCAP Antibody

Catalog # Availability Size / Price Qty
Detection of Human/Mouse BCAP by Western Blot.
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Product Details
Citations (2)
Supplemental Products

Human/Mouse BCAP Antibody Summary

Species Reactivity
Human, Mouse
Detects endogenous human and mouse BCAP in Western blots.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant human BCAP
Accession # Q6ZUJ8
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse BCAP antibody by Western Blot. View Larger

Detection of Human/Mouse BCAP by Western Blot. Western blot shows lysates of MO7e human megakaryocytic leukemic cell line and L1.2 mouse pro-B cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse BCAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4857) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for BCAP at approximately 100 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BCAP

BCAP (B-cell adaptor for phosphoinositide 3-kinase (PI3K)) participates in linking the B cell antigen receptor (BCR) with the PI3K pathway. Tyrosine phosphorylation of BCAP by BCR-associated protein tyrosine kinases, such as Syk and Btk, generates binding sites for the p85 subunit of PI3K, resulting in activation of the PI3K pathway. Human and mouse BCAP contain 3 YXXM SH2 binding motifs for interaction with PI3K.

Long Name
B Cell Adaptor Protein
Entrez Gene IDs
118788 (Human); 83490 (Mouse); 294048 (Rat)
Alternate Names
1810044J04Rik; B cell adaptor protein; BCAP; BCAPB-cell adapter for phosphoinositide 3-kinase; B-cell phosphoinositide 3-kinase adapter protein 1; FLJ35564; phosphoinositide 3-kinase adapter protein 1; phosphoinositide-3-kinase adaptor protein 1; Pik3ap1; RP11-34E5.3

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Citations for Human/Mouse BCAP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Negative Regulation of TLR Signaling by BCAP Requires Dimerization of Its DBB Domain
    Authors: JU Lauenstein, MJ Scherm, A Udgata, MC Moncrieffe, DI Fisher, NJ Gay
    J. Immunol., 2020;0(0):.
    Species: Escherichia coli
    Sample Types: Whole Cells
    Applications: ICC
  2. Phosphorylation of the multi functional signal transducer B-cell adaptor protein (BCAP) promotes recruitment of multiple SH2/SH3 proteins including GRB2
    Authors: JU Lauenstein, A Udgata, A Bartram, D De Sutter, DI Fisher, S Halabi, S Eyckerman, NJ Gay
    J. Biol. Chem., 2019;0(0):.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay


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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Goat IgG (H+L) Antibody


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