Human/Mouse BCAP Antibody

Catalog # Availability Size / Price Qty
AF4857
AF4857-SP
Detection of Human/Mouse BCAP by Western Blot.
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Product Details
Citations (2)
FAQs
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Human/Mouse BCAP Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects endogenous human and mouse BCAP in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human BCAP
Met462-Leu650
Accession # Q6ZUJ8
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse BCAP antibody by Western Blot. View Larger

Detection of Human/Mouse BCAP by Western Blot. Western blot shows lysates of MO7e human megakaryocytic leukemic cell line and L1.2 mouse pro-B cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse BCAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4857) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for BCAP at approximately 100 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BCAP

BCAP (B-cell adaptor for phosphoinositide 3-kinase (PI3K)) participates in linking the B cell antigen receptor (BCR) with the PI3K pathway. Tyrosine phosphorylation of BCAP by BCR-associated protein tyrosine kinases, such as Syk and Btk, generates binding sites for the p85 subunit of PI3K, resulting in activation of the PI3K pathway. Human and mouse BCAP contain 3 YXXM SH2 binding motifs for interaction with PI3K.

Long Name
B Cell Adaptor Protein
Entrez Gene IDs
118788 (Human); 83490 (Mouse); 294048 (Rat)
Alternate Names
1810044J04Rik; B cell adaptor protein; BCAP; BCAPB-cell adapter for phosphoinositide 3-kinase; B-cell phosphoinositide 3-kinase adapter protein 1; FLJ35564; phosphoinositide 3-kinase adapter protein 1; phosphoinositide-3-kinase adaptor protein 1; Pik3ap1; RP11-34E5.3

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Citations for Human/Mouse BCAP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Negative Regulation of TLR Signaling by BCAP Requires Dimerization of Its DBB Domain
    Authors: JU Lauenstein, MJ Scherm, A Udgata, MC Moncrieffe, DI Fisher, NJ Gay
    J. Immunol., 2020;0(0):.
    Species: Escherichia coli
    Sample Types: Whole Cells
    Applications: ICC
  2. Phosphorylation of the multi functional signal transducer B-cell adaptor protein (BCAP) promotes recruitment of multiple SH2/SH3 proteins including GRB2
    Authors: JU Lauenstein, A Udgata, A Bartram, D De Sutter, DI Fisher, S Halabi, S Eyckerman, NJ Gay
    J. Biol. Chem., 2019;0(0):.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Goat IgG (H+L) Antibody

WB,ELISA

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