Detects human and mouse Bcl‑w. Neutralizes recombinant human and mouse Bcl-w, but not recombinant human Bcl‑2 or Bcl‑x.
Monoclonal Mouse IgG1 Clone # 90336
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human Bcl-w Met1-Thr172 Accession # Q92843
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Measured by its ability to neutralize Recombinant Human Bcl-w (Catalog # 824-BW) inhibition of caspase-8-cleaved Recombinant Human BID (Catalog # 882-B8) induced release of cytochrome c from isolated mouse liver mitochondria using the Rat/Mouse Cytochrome c Quantikine ELISA Kit (Catalog # MCTC0).
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Bcl-w Inhibition of BID-induced Release of Cytochrome c and Neutralization by BCL-w Antibody. Cytochrome c released from enriched mouse liver mitochondria solubilized with Triton® X-100 was quantified with the Rat/Mouse Cytochrome c ELISA (Catalog # MCTC0).
Untreated: Release of Cytochrome c from untreated mitochondria
BID: Recombinant Human BID (Caspase-8-cleaved) (Catalog # 882-B8) induced release of Cytochrome c from mitochondria
BID + Bcl-w: Recombinant Human Bcl‑w (Catalog # 824-BW) inhibition of Recombinant Human BID (Caspase-8-cleaved) (Catalog # 882-B8) induced release of Cytochrome c from mitochondria
BID + Bcl-w + MAB8241: Mouse Anti-Human/Mouse Bcl‑w Monoclonal Antibody (Catalog # MAB8241) neutralized Recombinant Human Bcl‑w (Catalog # 824-BW) inhibition of Recombinant Human BID (Caspase-8-cleaved) (Catalog # 882-B8) induced release of Cytochrome c from mitochondria
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Bcl-w is a member of the Bcl-2 family of proteins that regulates outer mitochondrial membrane permeability (1, 2). Bcl-w is an anti-apoptotic member that prevents release of cytochrome c from the mitochondria intermembrane space into the cytosol. Bcl-w is required for normal sperm maturation (3, 4, 5). Natural Bcl-w contains a carboxyl-terminal mitochondria targeting sequence. Recombinant Bcl-w missing the mitochondrial targeting sequence maintains its ability to neutralize pro-apoptotic Bcl-2 family members. Neutralization by Bcl-w appears to be through binding the BH3 region of pro-apoptotic Bcl-2 family members. This activity does not require the mitochondrial targeting sequence.
Gross, A. et al. (1999) Genes and Develop. 13:1899.
Kroemer, G. (1997) Nature Med. 3:614.
Ross, J.A., et al. (1998) Nat. Genet. 18:251.
Print, C.G. et al. (1998) Proc. Natl. Acad. Sci. USA 95:12323.
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