Human/Mouse Bcl-w Antibody Summary
Accession # Q92843
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Bcl-w Inhibition of BID-induced Release of Cytochrome c and Neutralization by BCL-w Antibody.
Cytochrome c released from enriched mouse liver mitochondria solubilized with Triton®X-100 was quantified with the Rat/Mouse Cytochrome c ELISA (Catalog # MCTC0).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Bcl-w is a member of the Bcl-2 family of proteins that regulates outer mitochondrial membrane permeability (1, 2). Bcl-w is an anti-apoptotic member that prevents release of cytochrome c from the mitochondria intermembrane space into the cytosol. Bcl-w is required for normal sperm maturation (3, 4, 5). Natural Bcl-w contains a carboxyl-terminal mitochondria targeting sequence. Recombinant Bcl-w missing the mitochondrial targeting sequence maintains its ability to neutralize pro-apoptotic Bcl-2 family members. Neutralization by Bcl-w appears to be through binding the BH3 region of pro-apoptotic Bcl-2 family members. This activity does not require the mitochondrial targeting sequence.
- Gross, A. et al. (1999) Genes and Develop. 13:1899.
- Kroemer, G. (1997) Nature Med. 3:614.
- Ross, J.A., et al. (1998) Nat. Genet. 18:251.
Print, C.G. et al. (1998) Proc. Natl. Acad. Sci. USA 95:12323.
Yan, W. et al. (2000) Mol. Endocrin. 14:682.
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