Cytochrome c released from enriched mouse liver mitochondria was quantified with the Rat/Mouse Cytochrome c ELISA (Catalog # MCTC0). X ± SEM, n=3.
Total: Cytochrome c released from mouse liver mitochondria with Triton® X-100
Untreated: Cytochrome c released from untreated mitochondria
BID: Cytochrome c released from mitochondria when incubated with casp-8 cleaved human BID (Catalog # 882-B8)
BID + Bcl-w: Cytochrome c released from mitochondria when incubated with casp-8 cleaved human BID and human Bcl-w (Catalog # 824-BW)
BID + Bcl-w + MAB8241: Cytochrome c released from mitochondria when incubated with casp-8 cleaved human BID, human Bcl-w and Bcl-w neutralizing antibody
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Bcl-w is a member of the Bcl-2 family of proteins that regulates outer mitochondrial membrane permeability. Bcl-w is an anti-apoptotic member that prevents release of cytochrome c from the mitochondria intermembrane space into the cytosol. Bcl-w is required for normal sperm maturation. Natural Bcl-w contains a carboxyl-terminal mitochondria targeting sequence.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.