Human/Mouse Bcl-w Antibody

  
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse Bcl‑w. Neutralizes recombinant human and mouse Bcl-w, but not recombinant human Bcl‑2 or Bcl‑x.
  • Source
    Monoclonal Mouse IgG1 Clone # 90336
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human Bcl-w
    Met1-Thr172
    Accession # Q92843
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Neutralization
    Measured by its ability to neutralize Recombinant Human Bcl-w (Catalog # 824-BW) inhibition of caspase-8-cleaved Recombinant Human BID (Catalog # 882-B8) induced release of cytochrome c from isolated mouse liver mitochondria using the Rat/Mouse Cytochrome c Quantikine ELISA Kit (Catalog # MCTC0).
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Bcl-w Inhibition of BID-induced Release of Cytochrome c and Neutralization by BCL-w Antibody. Cytochrome c released from enriched mouse liver mitochondria solubilized with Triton® X-100 was quantified with the Rat/Mouse Cytochrome c ELISA (Catalog # MCTC0).

Untreated: Release of Cytochrome c from untreated mitochondria

BID: Recombinant Human BID (Caspase-8-cleaved) (Catalog # 882-B8) induced release of Cytochrome c from mitochondria

BID + Bcl-w: Recombinant Human Bcl‑w (Catalog # 824-BW) inhibition of Recombinant Human BID (Caspase-8-cleaved) (Catalog # 882-B8) induced release of Cytochrome c from mitochondria

BID + Bcl-w + MAB8241: Mouse Anti-Human/Mouse Bcl‑w Monoclonal Antibody (Catalog # MAB8241) neutralized Recombinant Human Bcl‑w (Catalog # 824-BW) inhibition of Recombinant Human BID (Caspase-8-cleaved) (Catalog # 882-B8) induced release of Cytochrome c from mitochondria

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Bcl-w
Bcl-w is a member of the Bcl-2 family of proteins that regulates outer mitochondrial membrane permeability (1, 2). Bcl-w is an anti-apoptotic member that prevents release of cytochrome c from the mitochondria intermembrane space into the cytosol. Bcl-w is required for normal sperm maturation (3, 4, 5). Natural Bcl-w contains a carboxyl-terminal mitochondria targeting sequence. Recombinant Bcl-w missing the mitochondrial targeting sequence maintains its ability to neutralize pro-apoptotic Bcl-2 family members. Neutralization by Bcl-w appears to be through binding the BH3 region of pro-apoptotic Bcl-2 family members. This activity does not require the mitochondrial targeting sequence.
  • References:
    1. Gross, A. et al. (1999) Genes and Develop. 13:1899.
    2. Kroemer, G. (1997) Nature Med. 3:614.
    3. Ross, J.A., et al. (1998) Nat. Genet. 18:251.
    4. Print, C.G. et al. (1998) Proc. Natl. Acad. Sci. USA 95:12323.
    5. Yan, W. et al. (2000) Mol. Endocrin. 14:682.
  • Long Name:
    B Cell Lymphoma/Leukemia w
  • Entrez Gene IDs:
    599 (Human); 12050 (Mouse)
  • Alternate Names:
    Apoptosis regulator Bcl-W; BCL2L2; Bcl2-L-2; BCL2-like 2; Bclw; Bcl-w; BCLWbcl-2-like protein 2; KIAA0271BCL2-L-2
Related Research Areas
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Secondary Antibodies
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Mouse IgG HRP-conjugated Antibody

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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse IgG Antibody

WB AF007 4
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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