Detection of Human/Mouse Bub3 by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, HeLa human cervical epithelial carcinoma cell line, 293T human embryonic kidney cell line, and NIH‑3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse Bub3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5706) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Bub3 at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Bub3 (Budding uninhibited by benzimidazoles 3 homolog; also Mitotic checkpoint protein Bub3) is a 35‑40 kDa member of the WD repeat Bub3 family of molecules. It plays a key role in chromosome segregation by serving as a component of the mitotic checkpoint complex. This complex inhibits Cdc20, and E3 ubiquitin ligase that initiates the metaphase to anaphase transition, until microtubules are properly aligned with, and bound to, chromatid kinetochores. Human Bub3 is 328 amino acids (aa) in length. It contains five WD40 repeats (aa 5‑43, 46‑83, 86‑124, 128‑163 and 223‑262) plus a phosphorylation site at Ser211 and a Lys at # 216 that may form an interchain crosslink with Gly. There are two splice variants that show two or four aa deletion over the four C‑terminal amino acids. Full‑length human and mouse Bub3 share 99% aa identity.
BUB3 Budding Uninhibited By Benzimidazoles 1 Homolog
Entrez Gene IDs:
9184 (Human); 12237 (Mouse)
BUB3 (budding uninhibited by benzimidazoles 3, yeast) homolog; BUB3 budding uninhibited by benzimidazoles 3 homolog; Bub3; BUB3L; budding uninhibited by benomyl; budding uninhibited by benzimidazoles 3 homolog (yeast); hBUB3; mitotic checkpoint component; mitotic checkpoint protein BUB3
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