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Human/Mouse CXCL12/SDF-1 Antibody

Catalog #: MAB310
43 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB310-SP
MAB310-100
MAB310-500
Chemotaxis Induced by CXCL12/SDF‑1 alpha and Neutral-ization by Human/Mouse CXCL12/SDF‑1 Antibody.
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Citations (43)
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Human/Mouse CXCL12/SDF-1 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse CXCL12/SDF‑1 alpha and human CXCL12/SDF‑1 beta.
Source
Monoclonal Mouse IgG1 Clone # 79014
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human CXCL12/SDF‑1 alpha
Lys22-Lys89
Accession # Q6ICW0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Neutralization

Measured by its ability to neutralize CXCL12/SDF‑1 alpha -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR4. At 111 μg/mL, this antibody will neutralize > 50% of the chemotactic effect due to 2 ng/mL Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF‑1 alpha.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Chemotaxis Induced by CXCL12/SDF‑1 alpha and Neutral-ization by Human/Mouse CXCL12/SDF‑1 Antibody. View Larger

Chemotaxis Induced by CXCL12/SDF‑1 alpha and Neutral-ization by Human/Mouse CXCL12/SDF‑1 Antibody. Recombinant Human/Feline/ Rhesus Macaque CXCL12/ SDF-1a (Catalog # 350-NS) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR4 in a dose-dependent manner (orange line). Chemotaxis elicited by Recombinant Human/Feline/ Rhesus Macaque CXCL12/ SDF-1a (2 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human/Mouse CXCL12/SDF-1 Monoclonal Antibody (Catalog # MAB310). At 111 µg/mL, this antibody will neutralize > 50% of the chemotactic effect.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL12/SDF-1

CXCL12, also known as SDF-1 (stromal cell-derived factor 1) and PBSF (Pre-B cell growth stimulatory factor), is a member of the CXC chemokine superfamily. Two alternatively spliced mRNAs encode CXCL12 alpha (89 aa residues) and CXCL12 beta (93 aa residues) that differ by the addition of 4 amino acids at the carboxy-terminus. Human and mouse CXCL12 are highly conserved. Mature human and mouse CXCL12 differ only by 1 amino acid.

Entrez Gene IDs
6387 (Human); 20315 (Mouse); 493806 (Feline); 574334 (Rhesus Macaque)
Alternate Names
chemokine (C-X-C motif) ligand 12; C-X-C motif chemokine 12; CXCL12; hIRH; hSDF-1; Intercrine reduced in hepatomas; IRH; PBSF; PBSFSDF-1a; Pre-B cell growth-stimulating factor; SCYB12; SDF1; SDF-1; SDF1A; SDF1B; SDF1SDF-1b; stromal cell-derived factor 1; TLSF; TLSF-a; TLSF-b; TPAR1

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Citations for Human/Mouse CXCL12/SDF-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

43 Citations: Showing 1 - 10
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  1. Tumor-infiltrated activated B cells suppress liver metastasis of colorectal cancers
    Authors: Y Xu, Z Wei, M Feng, D Zhu, S Mei, Z Wu, Q Feng, W Chang, M Ji, C Liu, Y Zhu, L Shen, F Yang, Y Chen, Y Feng, J Xu, D Zhu
    Cell Reports, 2022;40(9):111295.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  2. Engagement of the CXCL12-CXCR4 Axis in the Interaction of Endothelial Progenitor Cell and Smooth Muscle Cell to Promote Phenotype Control and Guard Vascular Homeostasis
    Authors: SF Mause, E Ritzel, A Deck, F Vogt, EA Liehn
    International Journal of Molecular Sciences, 2022;23(2):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  3. Plasma cell dynamics in the bone marrow niche
    Authors: Z Benet, Z Jing, DR Fooksman
    Cell Reports, 2021;34(6):108733.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  4. Combination of Fish Oil and Selenium Enhances Anticancer Efficacy and Targets Multiple Signaling Pathways in Anti-VEGF Agent Treated-TNBC Tumor-Bearing Mice
    Authors: CH Guo, S Hsia, CH Chung, YC Lin, MY Shih, PC Chen, GW Hsu, CT Fan, CL Peng
    Marine Drugs, 2021;19(4):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  5. Involvement of CXCL12/CXCR4 in the motility of human first-trimester endometrial epithelial cells through an autocrine mechanism by activating PI3K/AKT signaling
    Authors: J Zheng, D Qu, C Wang, L Ding, W Zhou
    BMC Pregnancy Childbirth, 2020;20(1):87.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  6. Reciprocal signaling and direct physical interactions between fibroblasts and breast cancer cells in a 3D environment
    Authors: DJ Wessels, N Pradhan, YN Park, MA Klepitsch, DF Lusche, KJ Daniels, KD Conway, ER Voss, SV Hegde, TP Conway, DR Soll
    PLoS ONE, 2019;14(6):e0218854.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  7. Indian Hedgehog Suppresses a Stromal Cell-Driven Intestinal Immune Response
    Authors: BF Westendorp, NVJA Büller, ON Karpus, WA van Dop, J Koster, R Versteeg, PJ Koelink, CY Snel, S Meisner, JJTH Roelofs, A Uhmann, E Ver Loren, J Heijmans, H Hahn, V Muncan, ME Wildenberg, GR van den Br
    Cell Mol Gastroenterol Hepatol, 2018;5(1):67-82.e1.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Periarteriolar Glioblastoma Stem Cell Niches Express Bone Marrow Hematopoietic Stem Cell Niche Proteins
    Authors: VVV Hira, JR Wormer, H Kakar, B Breznik, B van der Sw, R Hulsbos, W Tigchelaar, Z Tonar, M Khurshed, RJ Molenaar, CJF Van Noorde
    J. Histochem. Cytochem., 2018;0(0):2215541774917.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  9. Stromal Cell-Derived Factor 1 Mediates Immune Cell Attraction upon Urinary Tract Infection
    Authors: B Isaacson, T Hadad, A Glasner, C Gur, Z Granot, G Bachrach, O Mandelboim
    Cell Rep, 2017;20(1):40-47.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  10. Intrafibrillar silicified collagen scaffold modulates monocyte to promote cell homing, angiogenesis and bone regeneration
    Authors: Ji-Hua Chen
    Biomaterials, 2017;113(0):203-216.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  11. CYP4A in tumor-associated macrophages promotes pre-metastatic niche formation and metastasis
    Authors: XW Chen, TJ Yu, J Zhang, Y Li, HL Chen, GF Yang, W Yu, YZ Liu, XX Liu, CF Duan, HL Tang, M Qiu, CL Wang, H Zheng, J Yue, AM Guo, J Yang
    Oncogene, 2017;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Functional Assay
  12. Physiologically activated mammary fibroblasts promote postpartum mammary cancer
    Authors: Q Guo, J Minnier, J Burchard, K Chiotti, P Spellman, P Schedin
    JCI Insight, 2017;2(6):e89206.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  13. Hepatic carcinoma-associated fibroblasts enhance immune suppression by facilitating the generation of myeloid-derived suppressor cells
    Oncogene, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  14. Cathepsin K cleavage of SDF-1? inhibits its chemotactic activity towards glioblastoma stem-like cells
    Authors: Vashendriya V V Hira
    Biochim. Biophys. Acta, 2016;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  15. Natural nitration of CXCL12 reduces its signaling capacity and chemotactic activity in vitro and abrogates intra-articular lymphocyte recruitment in vivo
    Authors: R Janssens, A Mortier, D Boff, V Vanheule, M Gouwy, C Franck, O Larsen, MM Rosenkilde, J Van Damme, FA Amaral, MM Teixeira, S Struyf, P Proost
    Oncotarget, 2016;7(38):62439-62459.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Capture)
  16. Peritoneal Dissemination Requires an Sp1-Dependent CXCR4/CXCL12 Signaling Axis and Extracellular Matrix-Directed Spheroid Formation.
    Authors: Kasagi Y, Harada Y, Morodomi Y, Iwai T, Saito S, Yoshida K, Oki E, Saeki H, Ohgaki K, Sugiyama M, Onimaru M, Maehara Y, Yonemitsu Y
    Cancer Res, 2016;76(2):347-57.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  17. CXCR4+CD45- cells are niche forming for osteoclastogenesis via the SDF-1, CXCL7, and CX3CL1 signaling pathways in bone marrow
    Authors: Yoh Goto
    Stem Cells, 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  18. Lipopolysaccharide Promotes Choroidal Neovascularization by Up-Regulation of CXCR4 and CXCR7 Expression in Choroid Endothelial Cell.
    Authors: Feng Y, Guo H, Yuan F, Shen M
    PLoS ONE, 2015;10(8):e0136175.
    Species: Rat
    Sample Types: In Vivo
    Applications: Neutralization
  19. An important role of the SDF-1/CXCR4 axis in chronic skin inflammation.
    Authors: Zgraggen, Silvana, Huggenberger, Reto, Kerl, Katrin, Detmar, Michael
    PLoS ONE, 2014;9(4):e93665.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  20. IL-17-induced CXCL12 recruits B cells and induces follicle formation in BALT in the absence of differentiated FDCs.
    Authors: Fleige H, Ravens S, Moschovakis G, Bolter J, Willenzon S, Sutter G, Haussler S, Kalinke U, Prinz I, Forster R
    J Exp Med, 2014;211(4):643-51.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  21. SDF1-a facilitates Lin-/Sca1+ cell homing following murine experimental cerebral ischemia.
    Authors: Mocco J, Afzal A, Ansari S, Wolfe A, Caldwell K, Connolly E, Scott E
    PLoS ONE, 2014;9(1):e85615.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  22. CXCR4 and a cell-extrinsic mechanism control immature B lymphocyte egress from bone marrow.
    Authors: Beck T, Gomes A, Cyster J, Pereira J
    J Exp Med, 2014;211(13):2567-81.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  23. Vitamin D promotes vascular regeneration.
    Authors: Wong M, Leisegang M, Kruse C, Vogel J, Schurmann C, Dehne N, Weigert A, Herrmann E, Brune B, Shah A, Steinhilber D, Offermanns S, Carmeliet G, Badenhoop K, Schroder K, Brandes R
    Circulation, 2014;130(12):976-86.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  24. Platelet expression of stromal cell-derived factor-1 is associated with the degree of valvular aortic stenosis.
    Authors: Wurster T, Tegtmeyer R, Borst O, Rath D, Geisler T, Gawaz M, Bigalke B
    PLoS ONE, 2014;9(5):e97405.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  25. Hematopoietic stem cell cytokines and fibroblast growth factor-2 stimulate human endothelial cell-pericyte tube co-assembly in 3D fibrin matrices under serum-free defined conditions.
    Authors: Smith A, Bowers S, Stratman A, Davis G
    PLoS ONE, 2013;8(12):e85147.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  26. Inhibition of CXCR4-CXCL12 chemotaxis in melanoma by AMD11070.
    Authors: O'Boyle, G, Swidenbank, I, Marshall, H, Barker, C E, Armstrong, J, White, S A, Fricker, S P, Plummer, R, Wright, M, Lovat, P E
    Br J Cancer, 2013;108(8):1634-40.
  27. CXCL12-CXCR4 signaling is required for the maintenance of mouse spermatogonial stem cells.
    Authors: Yang, Qi-En, Kim, Dongwon, Kaucher, Amy, Oatley, Melissa, Oatley, Jon M
    J Cell Sci, 2013;126(0):1009-20.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  28. CXCR4 and CXCR7 regulate angiogenesis and CT26.WT tumor growth independent from SDF-1.
    Authors: Kollmar O, Rupertus K, Scheuer C, Nickels RM, Haberl GC, Tilton B, Menger MD, Schilling MK
    Int. J. Cancer, 2010;126(6):1302-15.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  29. Stimulation of chondrocyte hypertrophy by chemokine stromal cell-derived factor 1 in the chondro-osseous junction during endochondral bone formation.
    Authors: Wei L, Kanbe K, Lee M, Wei X, Pei M, Sun X, Terek R, Chen Q
    Dev. Biol., 2010;341(1):236-45.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  30. Stromal cell-derived factor-1 is essential for photoreceptor cell protection in retinal detachment.
    Authors: Otsuka H, Arimura N, Sonoda S
    Am. J. Pathol., 2010;177(5):2268-77.
    Species: Rat
    Sample Types: In Vivo
    Applications: Neutralization
  31. CXCL12 enhances exogenous CD4+CD25+ T cell migration and prevents embryo loss in non-obese diabetic mice.
    Authors: Lin Y, Xu L, Jin H, Zhong Y, Di J, Lin QD
    Fertil. Steril., 2009;91(6):2687-96.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  32. CXCL12 (SDF-1alpha) suppresses ongoing experimental autoimmune encephalomyelitis by selecting antigen-specific regulatory T cells.
    Authors: Meiron M, Zohar Y, Anunu R, Wildbaum G, Karin N
    J. Exp. Med., 2008;205(11):2643-55.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  33. Rapid chemotherapy-induced acute endothelial progenitor cell mobilization: implications for antiangiogenic drugs as chemosensitizing agents.
    Authors: Shaked Y, Henke E, Roodhart JM, Mancuso P, Langenberg MH, Colleoni M, Daenen LG, Man S, Xu P, Emmenegger U, Tang T, Zhu Z, Witte L, Strieter RM, Bertolini F, Voest EE, Benezra R, Kerbel RS
    Cancer Cell, 2008;14(3):263-73.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  34. CXCL12 is essential for migration of activated Langerhans cells from epidermis to dermis.
    Authors: Ouwehand K, Santegoets SJ, Bruynzeel DP, Scheper RJ, de Gruijl TD, Gibbs S
    Eur. J. Immunol., 2008;38(11):3050-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  35. Activation of bone marrow-derived microglia promotes photoreceptor survival in inherited retinal degeneration.
    Authors: Sasahara M, Otani A, Oishi A, Kojima H, Yodoi Y, Kameda T, Nakamura H, Yoshimura N
    Am. J. Pathol., 2008;172(6):1693-703.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  36. Cloning and expression pattern of dog SDF-1 and the implications of altered expression of SDF-1 in ischemic myocardium.
    Authors: Wei YJ, Tang Y, Li J, Cui CJ, Zhang H, Zhang XL, Zhang H, Hu SS
    Cytokine, 2007;40(1):52-9.
    Species: Canine
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  37. Osteoblasts support B-lymphocyte commitment and differentiation from hematopoietic stem cells.
    Authors: Zhu J, Garrett R, Jung Y, Zhang Y, Kim N, Wang J, Joe GJ, Hexner E, Choi Y, Taichman RS, Emerson SG
    Blood, 2007;109(9):3706-12.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  38. Pulmonary stromal-derived factor-1 expression and effect on neutrophil recruitment during acute lung injury.
    Authors: Petty JM, Sueblinvong V, Lenox CC, Jones CC, Cosgrove GP, Cool CD, Rai PR, Brown KK, Weiss DJ, Poynter ME, Suratt BT
    J. Immunol., 2007;178(12):8148-57.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  39. The role of CCL21 in recruitment of T-precursor cells to fetal thymi.
    Authors: Ueno T, Kuse S, Saito F, Nitta T, Kakiuchi T, Hollander GA, Takahama Y
    Blood, 2005;105(1):31-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  40. SDF-1 is both necessary and sufficient to promote proliferative retinopathy.
    Authors: Butler JM, Guthrie SM, Koc M, Afzal A, Caballero S, Brooks HL, Mames RN, Segal MS, Grant MB, Scott EW
    J. Clin. Invest., 2005;115(1):86-93.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  41. CXCR4 enhances adhesion of B16 tumor cells to endothelial cells in vitro and in vivo via beta(1) integrin.
    Authors: Cardones AR, Murakami T, Hwang ST
    Cancer Res., 2003;63(20):6751-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  42. Serum inactivation contributes to the failure of stromal-derived factor-1 to block HIV-I infection in vivo.
    Authors: Villalba S, Salvucci O, Aoki Y, De La Luz Sierra M, Gupta G, Davis D, Wyvill K, Little R, Yarchoan R, Tosato G
    J. Leukoc. Biol., 2003;74(5):880-8.
    Species: Human
    Sample Types: Serum
    Applications: Immunoprecipitation
  43. Mesenchymal Stem Cells Ameliorate Atherosclerotic Lesions via Restoring Endothelial Function.
    Authors: Lin Y, Yet S, Hsu Y, Wang G, Hung S
    Stem Cells Transl Med, 0;4(1):44-55.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization

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