Human/Mouse CXCL12/SDF-1 Antibody

(33 citations)   
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse CXCL12/SDF‑1 alpha and human CXCL12/SDF‑1 beta.
  • Source
    Monoclonal Mouse IgG1 Clone # 79014
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human CXCL12/SDF‑1 alpha
    Lys22-Lys89
    Accession # Q6ICW0
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Neutralization

    Measured by its ability to neutralize CXCL12/SDF‑1 alpha -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR4. At 111 μg/mL, this antibody will neutralize > 50% of the chemotactic effect due to 2 ng/mL Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF‑1 alpha.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Chemotaxis Induced by CXCL12/SDF‑1 alpha and Neutral­ization by Human/Mouse CXCL12/SDF‑1 Antibody. Recombinant Human/Feline/
Rhesus Macaque CXCL12/
SDF‑1 alpha (Catalog # 350-NS) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR4 in a dose-dependent manner (orange line). Chemotaxis elicited by Recombinant Human/Feline/
Rhesus Macaque CXCL12/
SDF‑1 alpha (2 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human/Mouse CXCL12/SDF‑1 Monoclonal Antibody (Catalog # MAB310). At 111 μg/mL, this antibody will neutralize  50% of the chemotactic effect.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCL12/SDF-1

CXCL12, also known as SDF-1 (stromal cell-derived factor 1) and PBSF (Pre-B cell growth stimulatory factor), is a member of the CXC chemokine superfamily. Two alternatively spliced mRNAs encode CXCL12 alpha (89 aa residues) and CXCL12 beta (93 aa residues) that differ by the addition of 4 amino acids at the carboxy-terminus. Human and mouse CXCL12 are highly conserved. Mature human and mouse CXCL12 differ only by 1 amino acid.

  • Entrez Gene IDs:
    6387 (Human); 20315 (Mouse); 493806 (Feline); 574334 (Rhesus Macaque)
  • Alternate Names:
    chemokine (C-X-C motif) ligand 12; C-X-C motif chemokine 12; CXCL12; hIRH; hSDF-1; Intercrine reduced in hepatomas; IRH; PBSF; PBSFSDF-1a; Pre-B cell growth-stimulating factor; SCYB12; SDF1; SDF-1; SDF1A; SDF1B; SDF1SDF-1b; stromal cell-derived factor 1; TLSF; TLSF-a; TLSF-b; TPAR1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

33 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Stromal Cell-Derived Factor 1 Mediates Immune Cell Attraction upon Urinary Tract Infection
    Authors: B Isaacson, T Hadad, A Glasner, C Gur, Z Granot, G Bachrach, O Mandelboim
    Cell Rep, 2017;20(1):40-47.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut
  2. Physiologically activated mammary fibroblasts promote postpartum mammary cancer
    Authors: Q Guo, J Minnier, J Burchard, K Chiotti, P Spellman, P Schedin
    JCI Insight, 2017;2(6):e89206.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  3. CYP4A in tumor-associated macrophages promotes pre-metastatic niche formation and metastasis
    Authors: XW Chen, TJ Yu, J Zhang, Y Li, HL Chen, GF Yang, W Yu, YZ Liu, XX Liu, CF Duan, HL Tang, M Qiu, CL Wang, H Zheng, J Yue, AM Guo, J Yang
    Oncogene, 2017;0(0):.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: Functional Assay
  4. CXCR4+CD45- cells are niche forming for osteoclastogenesis via the SDF-1, CXCL7, and CX3CL1 signaling pathways in bone marrow
    Authors: Yoh Goto
    Stem Cells, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  5. Hepatic carcinoma-associated fibroblasts enhance immune suppression by facilitating the generation of myeloid-derived suppressor cells
    Oncogene, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  6. Peritoneal Dissemination Requires an Sp1-Dependent CXCR4/CXCL12 Signaling Axis and Extracellular Matrix-Directed Spheroid Formation.
    Authors: Kasagi Y, Harada Y, Morodomi Y, Iwai T, Saito S, Yoshida K, Oki E, Saeki H, Ohgaki K, Sugiyama M, Onimaru M, Maehara Y, Yonemitsu Y
    Cancer Res, 2016;76(2):347-57.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut
  7. Natural nitration of CXCL12 reduces its signaling capacity and chemotactic activity in vitro and abrogates intra-articular lymphocyte recruitment in vivo
    Authors: R Janssens, A Mortier, D Boff, V Vanheule, M Gouwy, C Franck, O Larsen, MM Rosenkilde, J Van Damme, FA Amaral, MM Teixeira, S Struyf, P Proost
    Oncotarget, 2016;7(38):62439-62459.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Capture
  8. Lipopolysaccharide Promotes Choroidal Neovascularization by Up-Regulation of CXCR4 and CXCR7 Expression in Choroid Endothelial Cell.
    Authors: Feng Y, Guo H, Yuan F, Shen M
    PLoS ONE, 2015;10(8):e0136175.
    Species: Rat
    Sample Type: In Vivo
    Application: Neut
  9. Platelet expression of stromal cell-derived factor-1 is associated with the degree of valvular aortic stenosis.
    Authors: Wurster T, Tegtmeyer R, Borst O, Rath D, Geisler T, Gawaz M, Bigalke B
    PLoS ONE, 2014;9(5):e97405.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay
  10. SDF1-a facilitates Lin-/Sca1+ cell homing following murine experimental cerebral ischemia.
    Authors: Mocco J, Afzal A, Ansari S, Wolfe A, Caldwell K, Connolly E, Scott E
    PLoS ONE, 2014;9(1):e85615.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  11. An important role of the SDF-1/CXCR4 axis in chronic skin inflammation.
    Authors: Zgraggen, Silvana, Huggenberger, Reto, Kerl, Katrin, Detmar, Michael
    PLoS ONE, 2014;9(4):e93665.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut
  12. IL-17-induced CXCL12 recruits B cells and induces follicle formation in BALT in the absence of differentiated FDCs.
    Authors: Fleige H, Ravens S, Moschovakis G, Bolter J, Willenzon S, Sutter G, Haussler S, Kalinke U, Prinz I, Forster R
    J Exp Med, 2014;211(4):643-51.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  13. Vitamin D promotes vascular regeneration.
    Authors: Wong M, Leisegang M, Kruse C, Vogel J, Schurmann C, Dehne N, Weigert A, Herrmann E, Brune B, Shah A, Steinhilber D, Offermanns S, Carmeliet G, Badenhoop K, Schroder K, Brandes R
    Circulation, 2014;130(12):976-86.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  14. CXCR4 and a cell-extrinsic mechanism control immature B lymphocyte egress from bone marrow.
    Authors: Beck T, Gomes A, Cyster J, Pereira J
    J Exp Med, 2014;211(13):2567-81.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  15. Inhibition of CXCR4-CXCL12 chemotaxis in melanoma by AMD11070.
    Authors: O'Boyle, G, Swidenbank, I, Marshall, H, Barker, C E, Armstrong, J, White, S A, Fricker, S P, Plummer, R, Wright, M, Lovat, P E
    Br J Cancer, 2013;108(8):1634-40.
  16. CXCL12-CXCR4 signaling is required for the maintenance of mouse spermatogonial stem cells.
    Authors: Yang, Qi-En, Kim, Dongwon, Kaucher, Amy, Oatley, Melissa, Oatley, Jon M
    J Cell Sci, 2013;126(0):1009-20.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  17. Hematopoietic stem cell cytokines and fibroblast growth factor-2 stimulate human endothelial cell-pericyte tube co-assembly in 3D fibrin matrices under serum-free defined conditions.
    Authors: Smith A, Bowers S, Stratman A, Davis G
    PLoS ONE, 2013;8(12):e85147.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  18. Stromal cell-derived factor-1 is essential for photoreceptor cell protection in retinal detachment.
    Authors: Otsuka H, Arimura N, Sonoda S
    Am. J. Pathol., 2010;177(5):2268-77.
    Species: Rat
    Sample Type: In Vivo
    Application: In vivo
  19. CXCR4 and CXCR7 regulate angiogenesis and CT26.WT tumor growth independent from SDF-1.
    Authors: Kollmar O, Rupertus K, Scheuer C, Nickels RM, Haberl GC, Tilton B, Menger MD, Schilling MK
    Int. J. Cancer, 2010;126(6):1302-15.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  20. Stimulation of chondrocyte hypertrophy by chemokine stromal cell-derived factor 1 in the chondro-osseous junction during endochondral bone formation.
    Authors: Wei L, Kanbe K, Lee M, Wei X, Pei M, Sun X, Terek R, Chen Q
    Dev. Biol., 2010;341(1):236-45.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  21. CXCL12 enhances exogenous CD4+CD25+ T cell migration and prevents embryo loss in non-obese diabetic mice.
    Authors: Lin Y, Xu L, Jin H, Zhong Y, Di J, Lin QD
    Fertil. Steril., 2009;91(6):2687-96.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  22. Rapid chemotherapy-induced acute endothelial progenitor cell mobilization: implications for antiangiogenic drugs as chemosensitizing agents.
    Authors: Shaked Y, Henke E, Roodhart JM, Mancuso P, Langenberg MH, Colleoni M, Daenen LG, Man S, Xu P, Emmenegger U, Tang T, Zhu Z, Witte L, Strieter RM, Bertolini F, Voest EE, Benezra R, Kerbel RS
    Cancer Cell, 2008;14(3):263-73.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  23. CXCL12 is essential for migration of activated Langerhans cells from epidermis to dermis.
    Authors: Ouwehand K, Santegoets SJ, Bruynzeel DP, Scheper RJ, de Gruijl TD, Gibbs S
    Eur. J. Immunol., 2008;38(11):3050-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  24. CXCL12 (SDF-1alpha) suppresses ongoing experimental autoimmune encephalomyelitis by selecting antigen-specific regulatory T cells.
    Authors: Meiron M, Zohar Y, Anunu R, Wildbaum G, Karin N
    J. Exp. Med., 2008;205(11):2643-55.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  25. Activation of bone marrow-derived microglia promotes photoreceptor survival in inherited retinal degeneration.
    Authors: Sasahara M, Otani A, Oishi A, Kojima H, Yodoi Y, Kameda T, Nakamura H, Yoshimura N
    Am. J. Pathol., 2008;172(6):1693-703.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  26. Pulmonary stromal-derived factor-1 expression and effect on neutrophil recruitment during acute lung injury.
    Authors: Petty JM, Sueblinvong V, Lenox CC, Jones CC, Cosgrove GP, Cool CD, Rai PR, Brown KK, Weiss DJ, Poynter ME, Suratt BT
    J. Immunol., 2007;178(12):8148-57.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  27. Osteoblasts support B-lymphocyte commitment and differentiation from hematopoietic stem cells.
    Authors: Zhu J, Garrett R, Jung Y, Zhang Y, Kim N, Wang J, Joe GJ, Hexner E, Choi Y, Taichman RS, Emerson SG
    Blood, 2007;109(9):3706-12.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  28. Cloning and expression pattern of dog SDF-1 and the implications of altered expression of SDF-1 in ischemic myocardium.
    Authors: Wei YJ, Tang Y, Li J, Cui CJ, Zhang H, Zhang XL, Zhang H, Hu SS
    Cytokine, 2007;40(1):52-9.
    Species: Canine
    Sample Type: Tissue Homogenates
    Application: WB
  29. SDF-1 is both necessary and sufficient to promote proliferative retinopathy.
    Authors: Butler JM, Guthrie SM, Koc M, Afzal A, Caballero S, Brooks HL, Mames RN, Segal MS, Grant MB, Scott EW
    J. Clin. Invest., 2005;115(1):86-93.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  30. The role of CCL21 in recruitment of T-precursor cells to fetal thymi.
    Authors: Ueno T, Kuse S, Saito F, Nitta T, Kakiuchi T, Hollander GA, Takahama Y
    Blood, 2005;105(1):31-9.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  31. CXCR4 enhances adhesion of B16 tumor cells to endothelial cells in vitro and in vivo via beta(1) integrin.
    Authors: Cardones AR, Murakami T, Hwang ST
    Cancer Res., 2003;63(20):6751-7.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  32. Serum inactivation contributes to the failure of stromal-derived factor-1 to block HIV-I infection in vivo.
    Authors: Villalba S, Salvucci O, Aoki Y, De La Luz Sierra M, Gupta G, Davis D, Wyvill K, Little R, Yarchoan R, Tosato G
    J. Leukoc. Biol., 2003;74(5):880-8.
    Species: Human
    Sample Type: Serum
    Application: IP
  33. Mesenchymal Stem Cells Ameliorate Atherosclerotic Lesions via Restoring Endothelial Function.
    Authors: Lin Y, Yet S, Hsu Y, Wang G, Hung S
    Stem Cells Transl Med, 0;4(1):44-55.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
Expand to show all 33 Citations
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Isotype Control

Ctrl MAB002 138  
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Mouse IgG1 Isotype Control

Ctrl MAB002R 1
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse IgG Antibody

WB AF007 4
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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