Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all FoxJ3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human FoxJ3
Met375-Tyr552
Accession # NP_055762

Specificity

Detects human and mouse FoxJ3 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse FoxJ3 Antibody

Detection of Human FoxJ3 antibody by Western Blot.

Detection of Human FoxJ3 by Western Blot.

Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and Jurkat human acute T cell leukemia cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse FoxJ3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5786) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for FoxJ3 was detected at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse FoxJ3 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line and Jurkat human acute T cell leukemia cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FoxJ3

FoxJ3 (Forkhead box protein J3) is a 69 kDa (predicted) nuclear protein that is a Class 1 member of the Fox family of transcription factors. It is expressed in embryonic neuroectoderm, neural crest and somitic condensations, and is suggested to participate in terminal tissue differentiation. Human FoxJ3 is 622 amino acids (aa) in length. It contains one forkhead DNA binding region (aa 77‑168) plus a phosphorylation site at Ser223. There are two potential splice variants. One shows a deletion of aa 177‑210, while a second shows a 12 aa substitution for aa 177‑622. Over aa 375‑552, human FoxJ3 shares 93% aa identity with mouse FoxJ3.

Long Name

Forkhead Box J3

Alternate Names

forkhead box J3, forkhead box protein J3, KIAA1041, MGC165036, MGC176686

Entrez Gene IDs

22887 (Human); 230700 (Mouse); 313554 (Rat)

Gene Symbol

FOXJ3

UniProt

NP_055762

Additional FoxJ3 Products

Product Documents for Human/Mouse FoxJ3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse FoxJ3 Antibody

For research use only

Related Research Areas

Early Ectodermal Lineage Markers

Citations for Human/Mouse FoxJ3 Antibody

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