Human/Mouse FoxJ3 Antibody

Catalog # Availability Size / Price Qty
Detection of Human FoxJ3 by Western Blot.
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Product Details
Supplemental Products

Human/Mouse FoxJ3 Antibody Summary

Species Reactivity
Human, Mouse
Detects human and mouse FoxJ3 in Western blots.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant human FoxJ3
Accession # NP_055762
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human FoxJ3 antibody by Western Blot. View Larger

Detection of Human FoxJ3 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and Jurkat human acute T cell leukemia cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse FoxJ3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5786) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for FoxJ3 was detected at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FoxJ3

FoxJ3 (Forkhead box protein J3) is a 69 kDa (predicted) nuclear protein that is a Class 1 member of the Fox family of transcription factors. It is expressed in embryonic neuroectoderm, neural crest and somitic condensations, and is suggested to participate in terminal tissue differentiation. Human FoxJ3 is 622 amino acids (aa) in length. It contains one forkhead DNA binding region (aa 77‑168) plus a phosphorylation site at Ser223. There are two potential splice variants. One shows a deletion of aa 177‑210, while a second shows a 12 aa substitution for aa 177‑622. Over aa 375‑552, human FoxJ3 shares 93% aa identity with mouse FoxJ3.

Long Name
Forkhead Box J3
Entrez Gene IDs
22887 (Human); 230700 (Mouse); 313554 (Rat)
Alternate Names
forkhead box J3; forkhead box protein J3; FoxJ3; KIAA1041; MGC165036; MGC176686

Product Datasheets

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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