Human/Mouse HSP10/EPF Antibody

Catalog #: AF3298
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3298
AF3298-SP

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Detection of Human/Mouse HSP10/EPF by Western Blot.

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse HSP10/EPF Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects endogenous human and mouse HSP10/EPF in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human HSP10/EPF
Ala2-Asp102
Accession # P61604

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human/Mouse HSP10/EPF by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, A431 human epithelial carcinoma cell line, and Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL Human/Mouse HSP10/EPF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3298) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for HSP10/EPF was detected at approximately 8-9 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 4.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HSP10/EPF

The heat shock proteins (HSPs) are a highly conserved family of stress response proteins. HSPs function primarily as molecular chaperones, facilitating the folding of other cellular proteins, preventing protein aggregation, or targeting improperly folded proteins to specific degradative pathways. HSP10, also known as HSPE1, chaperonin 10 and Early Pregnancy Factor (EPF), is a 10.8 kDa protein with an apparent molecular weight of 8‑9 kDa that exists intracellularly as a 7 subunit multimer and assists HSP60 in folding non-native proteins.

Long Name

Heat Shock 10 kDa Protein 1

Entrez Gene IDs

3336 (Human); 69253 (Mouse)

Alternate Names

10 kDa chaperonin; 10 kDa heat shock protein, mitochondrial; Chaperonin 10 Homolog; Chaperonin 10; CPN10HSP10; Early-pregnancy factor; EPF; GroES; heat shock 10kD protein 1 (chaperonin 10); heat shock 10kDa protein 1 (chaperonin 10); HSP10; HSPE1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human/Mouse HSP10/EPF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Circulating heat shock protein 60 levels are elevated in HIV patients and are reduced by anti-retroviral therapy.
Authors: Anraku, Itaru, Rajasuriar, Reena, Dobbin, Caroline, Brown, Richard, Lewin, Sharon R, Suhrbier, Andreas
PLoS ONE, 2012;7(9):e45291.
Species: Human
Sample Types: Plasma
Applications: ELISA Development