Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Western Blot

Cited:

ELISA Development

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all HSP10/EPF Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human HSP10/EPF
Ala2-Asp102
Accession # P61604

Specificity

Detects endogenous human and mouse HSP10/EPF in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse HSP10/EPF Antibody

Detection of Human/Mouse HSP10/EPF antibody by Western Blot.

Detection of Human/Mouse HSP10/EPF by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line, A431 human epithelial carcinoma cell line, and Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL Human/Mouse HSP10/EPF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3298) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for HSP10/EPF was detected at approximately 8-9 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 4.

Applications for Human/Mouse HSP10/EPF Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line, A431 human epithelial carcinoma cell line, and Neuro-2A mouse neuroblastoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HSP10/EPF

The heat shock proteins (HSPs) are a highly conserved family of stress response proteins. HSPs function primarily as molecular chaperones, facilitating the folding of other cellular proteins, preventing protein aggregation, or targeting improperly folded proteins to specific degradative pathways. HSP10, also known as HSPE1, chaperonin 10 and Early Pregnancy Factor (EPF), is a 10.8 kDa protein with an apparent molecular weight of 8‑9 kDa that exists intracellularly as a 7 subunit multimer and assists HSP60 in folding non-native proteins.

Long Name

Heat Shock 10 kDa Protein 1

Alternate Names

Chaperonin 10 Homolog, EPF, GroES, HSPE1

Entrez Gene IDs

3336 (Human); 69253 (Mouse)

Gene Symbol

HSPE1

UniProt

P61604

Additional HSP10/EPF Products

Product Documents for Human/Mouse HSP10/EPF Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse HSP10/EPF Antibody

For research use only

Related Research Areas

Heat Shock Proteins (HSPs)

Citations for Human/Mouse HSP10/EPF Antibody

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