Human/Mouse MBD4 Antibody

Catalog # Availability Size / Price Qty
Detection of Human and Mouse MBD4 by Western Blot.
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Product Details
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Human/Mouse MBD4 Antibody Summary

Species Reactivity
Human, Mouse
Detects human and mouse MBD4 in Western blots.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant human MBD4
Accession # O95243
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human and Mouse MBD4 antibody by Western Blot. View Larger

Detection of Human and Mouse MBD4 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Raji human Burkitt's lymphoma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse MBD4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5935) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for MBD4 at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MBD4

MBD4 (Methyl CpG-binding domain protein 4; also MED1) is a 65-68 kDa member of the methyl CpG binding protein family. It is expressed in non-germline cells, and serves at least two functions: one, it interacts with FADD to up- or down-regulate apoptosis, and two, it acts as a DNA N-glycosylase that excises improper chromosomal thymines that are created through the spontaneous deamination of methylcytosine to thymine. Human MDB4 is 580 amino acids (aa) in length. It contains one DNA-binding MBD domain (aa 76-148) and one endonuclease domain (aa 455-580). There are three isoform variants. One shows a deletion of aa 395-400, another shows an AlaPro substitution for aa 539-580, and a third shows a Gln substitution for aa 83-401. Over aa 382-580, human MBD4 shares 90% aa identity with mouse MBD4.

Long Name
Methyl-CpG Binding Domain Protein 4
Entrez Gene IDs
8930 (Human); 17193 (Mouse); 680915 (Rat)
Alternate Names
EC 3.2.2.-; G/T mismatch glycosylase; G/U mismatch glycosylase; MBD4; MED1G/5-fluorouracil mismatch glycosylase with biphasic kinetics; methyl-CpG binding domain protein 4; methyl-CpG-binding domain protein 43,N(4)-ethenocytosine glycosylase; Methyl-CpG-binding endonuclease 1; Methyl-CpG-binding protein MBD4; Mismatch-specific DNA N-glycosylase; putative methyl-CpG binding protein

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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