Human/Mouse MMP-24/MT5-MMP Antibody

  
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse MMP‑24/MT5-MMP in Western blots.
  • Source
    Polyclonal Rabbit IgG
  • Purification
    Protein A or G purified
  • Immunogen
    E. coli-derived recombinant human MMP‑24/MT5-MMP
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human/Mouse MMP‑24/MT5-MMP Western Blot Standard (Catalog # WBC014)
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Immunoprecipitation
    25 µg/mL
    Conditioned cell culture medium spiked with Recombinant Human or Recombinant Mouse MMP-24/MT5-MMP, see our available Western blot detection antibodies
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
MMP‑24 in Human Brain. MMP‑24 was detected in immersion fixed paraffin-embedded sections of human brain using Rabbit Anti-Human/Mouse MMP-24/MT5-MMP Polyclonal Antibody (Catalog # AB924) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS006) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Immunohistochemistry
MMP‑24 in Human Astro­cytoma. MMP‑24 was detected in immersion fixed paraffin-embedded sections of human brain (astrocytoma) using Rabbit Anti-Human/Mouse MMP‑24/
MT5-MMP Polyclonal Antibody (Catalog # AB924) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS006) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MMP-24/MT5-MMP

The matrix metalloproteinases (MMPs) consist of 24 known human zinc proteases with essential roles in breaking down components of the extracellular matrix (ECM). Additional MMP substrates include cytokines, chemokines, growth factors and binding proteins, cell/cell adhesion molecules, and other proteinases. With a few exceptions, MMPs share common structural motifs including a pro-peptide domain, a catalytic domain, a hinge region, and a hemopexin-like domain. Synthesized as pro-enzymes, most MMPs are secreted before conversion to their active form. MMP activities are modulated on several levels including transcription, pro-enzyme activation, or by their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs). A subset of MMPs are associated with membranes and designated as membrane-type metalloproteinases (MT-MMP).

  • Long Name:
    Matrix Metalloproteinase 24/Membrane Type 5 MMP
  • Entrez Gene IDs:
    10893 (Human); 17391 (Mouse)
  • Alternate Names:
    EC 3.4.24; EC 3.4.24.-; EC 3.4.24.80; matrix metallopeptidase 24 (membrane-inserted); matrix metalloproteinase 24 (membrane-inserted); matrix metalloproteinase-24; membrane-type 5 matrix metalloproteinase; Membrane-type matrix metalloproteinase 5; Membrane-type-5 matrix metalloproteinase; MMP24; MMP-24; MT5MMP; MT5-MMP; MT5-MMPMMP25; MT-MMP 5; MTMMP5; MT-MMP5
Related Research Areas
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VisUCyte Antigen Retrieval Reagent-Universal

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Isotype Controls
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Normal Rabbit IgG Control

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Normal Rabbit IgG Control

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Secondary Antibodies
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Rabbit IgG HRP-conjugated Antibody

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Donkey Anti-Rabbit IgG NorthernLights™ NL557-conjugated Antibody

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Donkey Anti-Rabbit IgG NorthernLights™ NL637-conjugated Antibody

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Rabbit IgG APC-conjugated Antibody

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Rabbit IgG PE-conjugated Antibody

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Rabbit IgG Fluorescein-conjugated Antibody

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Donkey Anti-Rabbit IgG NorthernLights™ NL493-conjugated Antibody

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Goat Anti-Rabbit IgG Biotinylated Antibody

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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

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Rabbit IgG Antibody

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Rabbit IgG VisUCyte HRP Polymer Antibody

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Donkey Anti-Rabbit IgG (H+L) Antibody

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