Human/Mouse Myeloperoxidase/MPO Antibody

(13 citations)   
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse Myeloperoxidase/MPO in Western blots. Goat anti-human/mouse MPO does not cross‑react with recombinant human Eosinophil Peroxidase (EPPO) in Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse MPO
    Met16-Thr718
    Accession # P11247
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
  • Simple Western
    5 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry

Myeloperoxidase/MPO in Human Spleen. Myeloperoxidase/MPO was detected in immersion fixed paraffin-embedded sections of human spleen using 3 µg/mL Goat Anti-Human/Mouse Myeloperoxidase/MPO Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3667) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human/Mouse Myeloperoxidase/MPO by Western Blot. Western blot shows lysates of HL‑60 human acute promyelocytic leukemia cell line, human neutrophil cells, and mouse spleen tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse Myeloperoxidase/MPO Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3667) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Myeloperoxidase/MPO at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Detection of Mouse Myeloperoxidase/MPO by Simple WesternTM. Simple Western lane view shows lysates of mouse spleen tissue, loaded at 0.2 mg/mL. A specific band was detected for Myeloperoxidase/MPO at approximately 67 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse Myeloperoxidase/MPO Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3667) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.

Immunocytochemistry

Myeloperoxidase/MPO in Mouse Splenocytes. Myeloperoxidase/MPO was detected in immersion fixed mouse splenocytes using Goat Anti-Human/Mouse Myeloperoxidase/MPO Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3667) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Myeloperoxidase/MPO
Myeloperoxidase (MPO) is a hemeprotein that belongs to the XPO subfamily of the heme peroxidase superfamily. MPO is synthesized as a preproprotein that undergoes proteolytic processing to generate a disulfide-linked heterodimer of the N-terminal beta -subunit (12 kDa) and C-terminal alpha subunit (60 kDa). Active MPO is a tetramer of two beta -subunits and two alpha -subunits that are also disulfide-linked through the two alpha -subunits. MPO is stored in granules and is an abundant protein in neutrophils and monocytes. MPO is released upon activation to catalyze the formation of powerful oxidants such as hypochlorous acid, which kills microbes. Unprocessed pro-MPO can also be released. Mouse MPO shares 87% amino acid sequence identity with that of human MPO.
  • Entrez Gene IDs:
    4353 (Human); 17523 (Mouse); 303413 (Rat)
  • Alternate Names:
    EC 1.11.1; EC 1.11.1.7; Lactoperoxidase; MPO; Myeloperoxidase
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Host DNA released by NETosis promotes rhinovirus-induced type-2 allergic asthma exacerbation
    Authors: M Toussaint, DJ Jackson, D Swieboda, A Guedán, TD Tsouroukts, YM Ching, C Radermecke, H Makrinioti, J Aniscenko, MR Edwards, R Solari, F Farnir, V Papayannop, F Bureau, T Marichal, SL Johnston
    Nat. Med., 2017;23(6):681-691.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  2. The blood-borne sialyltransferase ST6Gal-1 is a negative systemic regulator of granulopoiesis
    Authors: CWL Dougher, A Buffone, MJ Nemeth, M Nasirikena, EE Irons, PN Bogner, JTY Lau
    J. Leukoc. Biol., 2017;0(0):.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  3. Topical Fibronectin Improves Wound Healing of Irradiated Skin
    Authors: MB Johnson, B Pang, DJ Gardner, S Niknam-Ben, V Soundaraja, A Bramos, DP Perrault, K Banks, GK Lee, RY Baker, GH Kim, S Lee, Y Chai, M Chen, W Li, L Kwong, YK Hong, AK Wong
    Sci Rep, 2017;7(1):3876.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  4. Neutrophil Attack Triggers Extracellular Trap-Dependent Candida Cell Wall Remodeling and Altered Immune Recognition
    PLoS Pathog, 2016;12(5):e1005644.
    Species: Mouse
    Sample Type: Whole Cells
    Application: IHC Not Specified
  5. Beneficial Effects of Sodium Phenylbutyrate Administration During Infection with Salmonella enterica serovar Typhimurium
    Infect Immun, 2016;0(0):.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  6. Swimming Motility Mediates the Formation of Neutrophil Extracellular Traps Induced by Flagellated Pseudomonas aeruginosa
    PLoS Pathog., 2016;12(11):e1005987.
    Species: Mouse
    Sample Type: Whole Cells
    Application: IF
  7. Rhadinovirus host entry by co-operative infection.
    Authors: Lawler C, Milho R, May J, Stevenson P
    PLoS Pathog, 2015;11(3):e1004761.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC OCT-embedded
  8. Correction: A chitin-like component on sclerotic cells of fonsecaea pedrosoi inhibits dectin-1-mediated murine Th17 development by masking beta-glucans.
    PLoS ONE, 2015;10(3):e0119244.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  9. The cytokine IL-22 promotes pathogen colonization by suppressing related commensal bacteria.
    Authors: Behnsen J, Jellbauer S, Wong C, Edwards R, George M, Ouyang W, Raffatellu M
    Immunity, 2014;40(2):262-73.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  10. Lactobacillus rhamnosus GG and Bifidobacterium longum attenuate lung injury and inflammatory response in experimental sepsis.
    Authors: Khailova L, Petrie B, Baird C, Dominguez Rieg J, Wischmeyer P
    PLoS ONE, 2014;9(5):e97861.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  11. IL-33 targeting attenuates intestinal mucositis and enhances effective tumor chemotherapy in mice.
    Authors: Guabiraba R, Besnard A, Menezes G, Secher T, Jabir M, Amaral S, Braun H, Lima-Junior R, Ribeiro R, Cunha F, Teixeira M, Beyaert R, Graham G, Liew F
    Mucosal Immunol, 2014;7(5):1079-93.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  12. Transcriptomic analysis of host immune and cell death responses associated with the influenza A virus PB1-F2 protein.
    Authors: Le Goffic R, Leymarie O, Chevalier C, Rebours E, Da Costa B, Vidic J, Descamps D, Sallenave JM, Rauch M, Samson M, Delmas B
    PLoS Pathog., 2011;7(8):e1002202.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  13. Tissue inhibitor of metalloproteinases 3 regulates resolution of inflammation following acute lung injury.
    Authors: Gill SE, Huizar I, Bench EM
    Am. J. Pathol., 2010;176(1):64-73.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: ELISA Development
Expand to show all 13 Citations
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