Human Myeloperoxidase/MPO Antibody (392105)

R&D Systems | Catalog # MAB3174

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Gerbil

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA Capture

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 392105
View all Myeloperoxidase/MPO Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Myeloperoxidase/MPO
Val279-Ser745
Accession # P05164

Specificity

Detects human Myeloperoxidase/MPO in Western blots. No cross-reactivity with recombinant human Eosinophil Peroxidase is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Myeloperoxidase/MPO Antibody (392105)

Detection of Human Myeloperoxidase/MPO antibody by Western Blot.

Detection of Human Myeloperoxidase/MPO by Western Blot.

Western blot shows lysates of HL-60 human acute promyelocytic leukemia cell line and human neutrophil. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Myeloperoxidase/MPO Monoclonal Antibody (Catalog # MAB3174) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Myeloperoxidase/MPO at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Myeloperoxidase/MPO antibody in MOLT-4 Human Cell Line by Immunocytochemistry (ICC).

Myeloperoxidase/MPO in MOLT-4 Human Cell Line.

Myeloperoxidase/ MPO was detected in immersion fixed MOLT-4 human acute lymphoblastic leukemia cell line using 8 µg/mL Mouse Anti-Human Myeloperoxidase/ MPO Monoclonal Antibody (Catalog # MAB3174) for 3 hours at room temperature. Cells were stained (red) and counter-stained (green). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Myeloperoxidase/MPO antibody in HL-60 Human Cell Line by Immunocytochemistry (ICC).

Myeloperoxidase/MPO in HL‑60 Human Cell Line.

Myeloperoxidase/MPO was detected in immersion fixed HL-60 human acute promyelocytic leukemia cell line using Mouse Anti-Human Myeloperoxidase/MPO Mono-clonal Antibody (Catalog # MAB3174) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conju-gated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Myeloperoxidase/MPO antibody in Human Spleen by Immunohistochemistry (IHC-P).

Myeloperoxidase/MPO in Human Spleen.

Myeloperoxidase/MPO was detected in immersion fixed paraffin-embedded sections of human spleen using 15 µg/mL Mouse Anti-Human Myeloperoxidase/MPO Monoclonal Antibody (Catalog # MAB3174) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Myeloperoxidase/MPO in Mouse Spleen.

Myeloperoxidase/MPO was detected in immersion fixed paraffin-embedded sections of mouse spleen using Mouse Anti-Human Myeloperoxidase/MPO Monoclonal Antibody (Catalog # MAB3174) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of Myeloperoxidase by Immunohistochemistry

Detection of Myeloperoxidase by Immunohistochemistry

Increased levels of MPO in patient tissue biopsies associated with SpeB− GAS. A Representative immunofluorescence micrographs of the distribution of MPO in patient biopsies are shown. B Image analyses of MPO and C GAS stainings. Calculation of the stained area (px2) per analyzed micrograph is presented. Each bar within diagrams denotes analysis of one image from indicated tissue biopsies. Bars on the right of each diagram denote mean values ± SD. The level of significance between the groups was determined using 2-tailed Mann–Whitney U test Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37430325), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Myeloperoxidase by Immunohistochemistry

Detection of Myeloperoxidase by Immunohistochemistry

NEC survival and intestinal injury.(A) Survival curves for mice pups from the 3 groups on postnatal days 5 to 9. (B) Representative histological images of the terminal ileum. (C) Histological score of intestinal injury. Inflammatory gene marker expression of (D) Il-6 and (E) Tnf-alpha. Immunofluorescent protein expression of (F) MPO and (G) CC3 in the terminal ilea of the 3 groups. Yellow arrows indicate positive staining. Relative quantification of the (H) MPO and (I) CC3 protein expressions. (J) Angiogenesis/vascularization Vegf gene expression in the 3 groups. Data are presented as mean ± standard error, with significance of group comparisons based on one-way ANOVA and Tukey post-hoc tests. n = 10 for each group, *p<0.05, **p<0.01, and ***p<0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34855833), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Myeloperoxidase by Immunohistochemistry

Detection of Myeloperoxidase by Immunohistochemistry

Pathological features and NET marker expression in metastatic ccRCC. (A) Tissue HE staining (B) Tissue H3Cit immunohistochemistry (IHC) staining (C) Tissue MPO IHC staining (80×, magnification 400×, scale bar: 200 μm) D. 800× magnified Immunofluorescence staining of NET in tumor tissue (scale bar: 25 μm) E. NET in matched tumor tissue and adjacent normal tissue (magnification 200× and 400×, scale bar: 50 μm, ***p < 0.001). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38018346), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Myeloperoxidase/MPO Antibody (392105)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed MOLT-4 human acute lymphoblastic leukemia cell line and HL-60 human acute promyelocytic leukemia cell line

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human spleen and mouse spleen

Western Blot

1 µg/mL
Sample: HL-60 human acute promyelocytic leukemia cell line and human neutrophil

Reviewed Applications

Read 5 reviews rated 3.4 using MAB3174 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Myeloperoxidase/MPO

Myeloperoxidase (MPO) is a hemeprotein that belongs to the XPO subfamily of the heme peroxidase superfamily. MPO is synthesized as a preproprotein that undergoes proteolytic processing to generate a disulfide-linked heterodimer of the N-terminal beta -subunit (12 kDa) and C-terminal alpha subunit (60 kDa). Active MPO is a tetramer of two beta -subunits and two alpha -subunits that are also disulfide-linked through the two alpha -subunits. MPO is stored in granules and is an abundant protein in neutrophils and monocytes. MPO is released upon activation to catalyze the formation of powerful oxidants such as hypochlorous acid, which kills microbes. Unprocessed pro-MPO can also be released. Human and mouse MPO share 87% amino acid sequence identity.

Alternate Names

MPO

Entrez Gene IDs

4353 (Human); 17523 (Mouse); 303413 (Rat)

Gene Symbol

MPO

UniProt

P05164

Additional Myeloperoxidase/MPO Products

Product Documents for Human Myeloperoxidase/MPO Antibody (392105)

Certificate of Analysis

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Product Specific Notices for Human Myeloperoxidase/MPO Antibody (392105)

For research use only

Citations for Human Myeloperoxidase/MPO Antibody (392105)

Customer Reviews for Human Myeloperoxidase/MPO Antibody (392105) (5)

3.4 out of 5
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Customer Images

Showing  1 - 5 of 5 reviews Showing All
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  • Human Myeloperoxidase/MPO Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Myocardia
    Species: Human
    Verified Customer | Posted 10/10/2021
    Human Myeloperoxidase/MPO Antibody (392105) MAB3174
  • Human Myeloperoxidase/MPO Antibody
    Name: Balaji Mahender
    Application: ELISA
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 12/20/2017
  • Human/Mouse Myeloperoxidase/MPO Antibody
    Name: Cynthia de Bont
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Peripheral blood neutrophils
    Species: Human
    Verified Customer | Posted 09/08/2016
    This antibody does not stain Neutrophil Extracellular Traps (NETs) on coverslips, whereas it does stain intracellular MPO. This raises questions about its specificity. This antibody is also not reactive in a sandwich ELISA to detect NETs, despite the fact that MPO is known to be a very abundant protein on NETs... On western blot it does detect a protein at the right molecular weight already at low concentrations.
    Bio-Techne Response
    R&D Systems Technical Service is investigating.
  • Human/Mouse Myeloperoxidase/MPO Antibody
    Name: Pragnya Das
    Application: Immunohistochemistry
    Sample Tested: Adult heart
    Species: Mouse
    Verified Customer | Posted 09/02/2016
    Used at a dilution of 1:50. Non-R-D secondary, biotinylated at a dilution of 1:200
    Human Myeloperoxidase/MPO Antibody (392105) MAB3174
  • Human/Mouse Myeloperoxidase/MPO Antibody
    Name: Vishal Singh
    Application: Western Blot
    Sample Tested: Colon tissue
    Species: Mouse
    Verified Customer | Posted 03/30/2016
    None

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