Human Myeloperoxidase/MPO Antibody Summary
Accession # P05164
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human Myeloperoxidase/MPO by Western Blot. Western blot shows lysates of HL-60 human acute promyelocytic leukemia cell line and human neutrophil. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Myeloperoxidase/MPO Monoclonal Antibody (Catalog # MAB3174) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Myeloperoxidase/MPO at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Myeloperoxidase/MPO in MOLT-4 Human Cell Line. Myeloperoxidase/ MPO was detected in immersion fixed MOLT-4 human acute lymphoblastic leukemia cell line using 8 µg/mL Mouse Anti-Human Myeloperoxidase/ MPO Monoclonal Antibody (Catalog # MAB3174) for 3 hours at room temperature. Cells were stained (red) and counter-stained (green). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Myeloperoxidase/MPO in HL‑60 Human Cell Line. Myeloperoxidase/MPO was detected in immersion fixed HL-60 human acute promyelocytic leukemia cell line using Mouse Anti-Human Myeloperoxidase/MPO Mono-clonal Antibody (Catalog # MAB3174) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conju-gated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Myeloperoxidase/MPO in Human Spleen. Myeloperoxidase/MPO was detected in immersion fixed paraffin-embedded sections of human spleen using 15 µg/mL Mouse Anti-Human Myeloperoxidase/MPO Monoclonal Antibody (Catalog # MAB3174) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Myeloperoxidase (MPO) is a hemeprotein that belongs to the XPO subfamily of the heme peroxidase superfamily. MPO is synthesized as a preproprotein that undergoes proteolytic processing to generate a disulfide-linked heterodimer of the N-terminal beta -subunit (12 kDa) and C-terminal alpha subunit (60 kDa). Active MPO is a tetramer of two beta -subunits and two alpha -subunits that are also disulfide-linked through the two alpha -subunits. MPO is stored in granules and is an abundant protein in neutrophils and monocytes. MPO is released upon activation to catalyze the formation of powerful oxidants such as hypochlorous acid, which kills microbes. Unprocessed pro-MPO can also be released. Human and mouse MPO share 87% amino acid sequence identity.
Citations for Human Myeloperoxidase/MPO Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 5
Filter your results:
The Traditional Chinese Medicine MLC901 inhibits inflammation processes after focal cerebral ischemia
Authors: C Widmann, C Gandin, A Petit-Pait, M Lazdunski, C Heurteaux
Sci Rep, 2018;8(1):18062.
Sample Types: Whole Tissue
Antimicrobial cathelicidin peptide LL?37 induces NET formation and suppresses the inflammatory response in a mouse septic model
Authors: H Hosoda, K Nakamura, Z Hu, H Tamura, J Reich, K Kuwahara-A, T Iba, Y Tabe, I Nagaoaka
Mol Med Rep, 2017;0(0):.
Suppression Colitis and Colitis-Associated Colon Cancer by Anti-S100a9 Antibody in Mice
Authors: X Zhang, L Wei, J Wang, Z Qin, J Wang, Y Lu, X Zheng, Q Peng, Q Ye, F Ai, P Liu, S Wang, G Li, S Shen, J Ma
Front Immunol, 2017;8(0):1774.
Sample Types: Whole Tissue
Myocardial Structural and Biological Anomalies Induced by High Fat Diet in Psammomys obesus Gerbils.
Authors: Sahraoui A, Dewachter C, de Medina G, Naeije R, Aouichat Bouguerra S, Dewachter L
PLoS ONE, 2016;11(2):e0148117.
Sample Types: Whole Tissue
The Ancient Immunoglobulin Domains of Peroxidasin Are Required to Form Sulfilimine Cross-links in Collagen IV.
Authors: Ero-Tolliver I, Hudson B, Bhave G
J Biol Chem, 2015;290(35):21741-8.
Sample Types: Cell Lysates
Applications: Western Blot
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Cell and Tissue Staining Kits
Reviews for Human Myeloperoxidase/MPO Antibody
Average Rating: 3 (Based on 4 Reviews)
Have you used Human Myeloperoxidase/MPO Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
This antibody does not stain Neutrophil Extracellular Traps (NETs) on coverslips, whereas it does stain intracellular MPO. This raises questions about its specificity. This antibody is also not reactive in a sandwich ELISA to detect NETs, despite the fact that MPO is known to be a very abundant protein on NETs... On western blot it does detect a protein at the right molecular weight already at low concentrations.
R&D Systems Technical Service is investigating.
Used at a dilution of 1:50. Non-R-D secondary, biotinylated at a dilution of 1:200