Human Myeloperoxidase/MPO Antibody

Catalog # Availability Size / Price Qty
MAB3174
MAB3174-SP
Human Myeloperoxidase/MPO Antibody in Data
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Product Details
Citations (5)
FAQs
Supplemental Products
Reviews (4)

Human Myeloperoxidase/MPO Antibody Summary

Species Reactivity
Human
Specificity
Detects human Myeloperoxidase/MPO in Western blots. No cross-reactivity with recombinant human Eosinophil Peroxidase is observed.
Source
Monoclonal Mouse IgG2B Clone # 392105
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Myeloperoxidase/MPO
Val279-Ser745
Accession # P05164
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human Myeloperoxidase/MPO by Western Blot. View Larger

Detection of Human Myeloperoxidase/MPO by Western Blot. Western blot shows lysates of HL-60 human acute promyelocytic leukemia cell line and human neutrophil. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Myeloperoxidase/MPO Monoclonal Antibody (Catalog # MAB3174) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Myeloperoxidase/MPO at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Immunocytochemistry Myeloperoxidase/MPO in MOLT-4 Human Cell Line. View Larger

Myeloperoxidase/MPO in MOLT-4 Human Cell Line. Myeloperoxidase/ MPO was detected in immersion fixed MOLT-4 human acute lymphoblastic leukemia cell line using 8 µg/mL Mouse Anti-Human Myeloperoxidase/ MPO Monoclonal Antibody (Catalog # MAB3174) for 3 hours at room temperature. Cells were stained (red) and counter-stained (green). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry Myeloperoxidase/MPO in HL‑60 Human Cell Line. View Larger

Myeloperoxidase/MPO in HL‑60 Human Cell Line. Myeloperoxidase/MPO was detected in immersion fixed HL-60 human acute promyelocytic leukemia cell line using Mouse Anti-Human Myeloperoxidase/MPO Mono-clonal Antibody (Catalog # MAB3174) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conju-gated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry Myeloperoxidase/MPO in Human Spleen. View Larger

Myeloperoxidase/MPO in Human Spleen. Myeloperoxidase/MPO was detected in immersion fixed paraffin-embedded sections of human spleen using 15 µg/mL Mouse Anti-Human Myeloperoxidase/MPO Monoclonal Antibody (Catalog # MAB3174) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Myeloperoxidase/MPO

Myeloperoxidase (MPO) is a hemeprotein that belongs to the XPO subfamily of the heme peroxidase superfamily. MPO is synthesized as a preproprotein that undergoes proteolytic processing to generate a disulfide-linked heterodimer of the N-terminal beta -subunit (12 kDa) and C-terminal alpha subunit (60 kDa). Active MPO is a tetramer of two beta -subunits and two alpha -subunits that are also disulfide-linked through the two alpha -subunits. MPO is stored in granules and is an abundant protein in neutrophils and monocytes. MPO is released upon activation to catalyze the formation of powerful oxidants such as hypochlorous acid, which kills microbes. Unprocessed pro-MPO can also be released. Human and mouse MPO share 87% amino acid sequence identity.

Entrez Gene IDs
4353 (Human); 17523 (Mouse); 303413 (Rat)
Alternate Names
EC 1.11.1; EC 1.11.1.7; Lactoperoxidase; MPO; Myeloperoxidase

Product Datasheets

Citations for Human Myeloperoxidase/MPO Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. The Traditional Chinese Medicine MLC901 inhibits inflammation processes after focal cerebral ischemia
    Authors: C Widmann, C Gandin, A Petit-Pait, M Lazdunski, C Heurteaux
    Sci Rep, 2018;8(1):18062.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Antimicrobial cathelicidin peptide LL?37 induces NET formation and suppresses the inflammatory response in a mouse septic model
    Authors: H Hosoda, K Nakamura, Z Hu, H Tamura, J Reich, K Kuwahara-A, T Iba, Y Tabe, I Nagaoaka
    Mol Med Rep, 2017;0(0):.
    Species: Human
    Sample Types:
  3. Suppression Colitis and Colitis-Associated Colon Cancer by Anti-S100a9 Antibody in Mice
    Authors: X Zhang, L Wei, J Wang, Z Qin, J Wang, Y Lu, X Zheng, Q Peng, Q Ye, F Ai, P Liu, S Wang, G Li, S Shen, J Ma
    Front Immunol, 2017;8(0):1774.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  4. Myocardial Structural and Biological Anomalies Induced by High Fat Diet in Psammomys obesus Gerbils.
    Authors: Sahraoui A, Dewachter C, de Medina G, Naeije R, Aouichat Bouguerra S, Dewachter L
    PLoS ONE, 2016;11(2):e0148117.
    Species: Gerbil
    Sample Types: Whole Tissue
    Applications: IHC-P
  5. The Ancient Immunoglobulin Domains of Peroxidasin Are Required to Form Sulfilimine Cross-links in Collagen IV.
    Authors: Ero-Tolliver I, Hudson B, Bhave G
    J Biol Chem, 2015;290(35):21741-8.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Myeloperoxidase/MPO Antibody

Average Rating: 3 (Based on 4 Reviews)

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Human Myeloperoxidase/MPO Antibody
By Anonymous on 12/20/2017
Application: ELISA Sample Tested: EDTA Plasma Species: Human

Human/Mouse Myeloperoxidase/MPO Antibody
By Anonymous on 09/08/2016
Application: ICC/IF Sample Tested: Peripheral blood neutrophils Species: Human

This antibody does not stain Neutrophil Extracellular Traps (NETs) on coverslips, whereas it does stain intracellular MPO. This raises questions about its specificity. This antibody is also not reactive in a sandwich ELISA to detect NETs, despite the fact that MPO is known to be a very abundant protein on NETs... On western blot it does detect a protein at the right molecular weight already at low concentrations.

R&D Systems Technical Service is investigating.


Human/Mouse Myeloperoxidase/MPO Antibody
By Anonymous on 09/02/2016
Application: IHC Sample Tested: Adult heart Species: Mouse

Used at a dilution of 1:50. Non-R-D secondary, biotinylated at a dilution of 1:200


Human/Mouse Myeloperoxidase/MPO Antibody
By Anonymous on 03/30/2016
Application: WB Sample Tested: Colon tissue Species: Mouse

None