Human/Mouse Neprilysin/CD10 Antibody Summary
Accession # Q61391
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Neprilysin/CD10 in Human Kidney. Neprilysin/CD10 was detected in immersion fixed paraffin-embedded sections of human kidney using Rat Anti-Human/Mouse Neprilysin/CD10 Monoclonal Antibody (Catalog # MAB1126) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to convoluted tubules and glomeruli. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of Human and Mouse Neprilysin/CD10 by Western Blot. Western blot shows lysates of LNCaP human prostate cancer cell line and PC-3 human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human/Mouse Neprilysin/CD10 Monoclonal Antibody (Catalog # MAB1126) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). For additional reference, Recombinant Mouse Neprilysin (Catalog # 1126-ZN) (5 and 50 ng/lane) and Recombinant Human Neprilysin (CHO-expressed) (Catalog # 1182-ZNC) (5 and 50 ng/lane) were included. Specific bands were detected for Neprilysin/CD10 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Neprilysin is also known as CALLA and CD10. It is a zinc metalloprotease expressed at the cell surface that cleaves a variety of bioactive peptides such as enkephalins, atrial natriuretic peptide and amyloid beta peptide.
Citations for Human/Mouse Neprilysin/CD10 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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Green Tea Seed Isolated Theasaponin E1 Ameliorates AD Promoting Neurotoxic Pathogenesis by Attenuating Abeta Peptide Levels in SweAPP N2a Cells
Authors: MI Khan, JH Shin, MY Kim, TS Shin, JD Kim
Sample Types: Cell Lysates
Applications: Western Blot
Polyhydroxycurcuminoids but not curcumin upregulate neprilysin and can be applied to the prevention of Alzheimer's disease
Sci Rep, 2016-07-13;6(0):29760.
Sample Types: Tissue Homogenates
Applications: Western Blot
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