Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all NeuroD2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human NeuroD2
Ser174-Asn382
Accession # Q15784

Specificity

Detects human NeuroD2 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human NeuroD1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse NeuroD2 Antibody

Detection of Mouse NeuroD2 antibody by Western Blot.

Detection of Mouse NeuroD2 by Western Blot.

Western blot shows lysates of mouse brain (cortex) tissue. PVDF Membrane was probed with 1 µg/mL of Human NeuroD2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2775) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for NeuroD2 at approximately 41 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human/Mouse NeuroD2 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Mouse brain (cortex) tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NeuroD2

NeuroD2 (Neurogenic differentiation factor 2; also NDRF and KW8) is a 41 kDa nuclear member of the neuroD family of transcription factors. It is expressed in developing and mature neurons such as hippocampal granular neurons, and acts (in part) to repress factors that would otherwise block multipotential cell commitment to a neuronal lineage. NeuroD2 is presumed to act as a heterodimer with other bHLH transcription factors. Human NeuroD2 is 382 amino acids (aa) in length. It contains a poly-Glu region (aa 82-91), an NLS (aa 107-113), a DNA-binding HLH domain (aa 119-178) and a poly-Gly segment (aa 282-285). Based on rat studies, human NeuroD2 will undergo variable phosphorylation in the C-terminal region. Over aa 174-382, human and mouse NeuroD2 possess identical amino acid sequences.

Long Name

Neurogenic Differentiation Factor 2

Alternate Names

NDRF

Entrez Gene IDs

4761 (Human)

Gene Symbol

NEUROD2

UniProt

Q15784

Additional NeuroD2 Products

Product Documents for Human/Mouse NeuroD2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse NeuroD2 Antibody

For research use only

Citations for Human/Mouse NeuroD2 Antibody

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