Human/Mouse NeuroD2 Antibody

Catalog # Availability Size / Price Qty
AF2775
AF2775-SP
Detection of Mouse NeuroD2 by Western Blot.
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Human/Mouse NeuroD2 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human NeuroD2 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human NeuroD1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human NeuroD2
Ser174-Asn382
Accession # Q15784
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Mouse NeuroD2 by Western Blot. View Larger

Detection of Mouse NeuroD2 by Western Blot. Western blot shows lysates of mouse brain (cortex) tissue. PVDF Membrane was probed with 1 µg/mL of Human NeuroD2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2775) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for NeuroD2 at approximately 41 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NeuroD2

NeuroD2 (Neurogenic differentiation factor 2; also NDRF and KW8) is a 41 kDa nuclear member of the neuroD family of transcription factors. It is expressed in developing and mature neurons such as hippocampal granular neurons, and acts (in part) to repress factors that would otherwise block multipotential cell commitment to a neuronal lineage. NeuroD2 is presumed to act as a heterodimer with other bHLH transcription factors. Human NeuroD2 is 382 amino acids (aa) in length. It contains a poly-Glu region (aa 82-91), an NLS (aa 107-113), a DNA-binding HLH domain (aa 119-178) and a poly-Gly segment (aa 282-285). Based on rat studies, human NeuroD2 will undergo variable phosphorylation in the C-terminal region. Over aa 174-382, human and mouse NeuroD2 possess identical amino acid sequences.

Long Name
Neurogenic Differentiation Factor 2
Entrez Gene IDs
4761 (Human)
Alternate Names
BHLHA1; bHLHa1neurogenic differentiation factor 2; Class A basic helix-loop-helix protein 1; MGC26304; NDRF; NDRFneurogenic basic-helix-loop-helix protein; NeuroD2; NeuroD-related factor; neurogenic differentiation 2

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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