Detection of Mouse NeuroD2 by Western Blot.
Western blot shows lysates of mouse brain (cortex) tissue. PVDF Membrane was probed with 1 µg/mL of Human NeuroD2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2775) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for NeuroD2 at approximately 41 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
NeuroD2 (Neurogenic differentiation factor 2; also NDRF and KW8) is a 41 kDa nuclear member of the neuroD family of transcription factors. It is expressed in developing and mature neurons such as hippocampal granular neurons, and acts (in part) to repress factors that would otherwise block multipotential cell commitment to a neuronal lineage. NeuroD2 is presumed to act as a heterodimer with other bHLH transcription factors. Human NeuroD2 is 382 amino acids (aa) in length. It contains a poly-Glu region (aa 82-91), an NLS (aa 107-113), a DNA-binding HLH domain (aa 119-178) and a poly-Gly segment (aa 282-285). Based on rat studies, human NeuroD2 will undergo variable phosphorylation in the C-terminal region. Over aa 174-382, human and mouse NeuroD2 possess identical amino acid sequences.
Neurogenic Differentiation Factor 2
Entrez Gene IDs:
BHLHA1; bHLHa1neurogenic differentiation factor 2; Class A basic helix-loop-helix protein 1; MGC26304; NDRF; NDRFneurogenic basic-helix-loop-helix protein; NeuroD2; NeuroD-related factor; neurogenic differentiation 2
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