Norrin, also known as Norrie Disease Protein, is a secreted protein that has a C-terminal cysteine-knot motif. Although Norrin is unrelated to Wnt family proteins, it is a Frizzled-4-specific ligand that activates the canonical Wnt pathway through Frizzled-4 and LRP5 (LDL receptor-related protein). Mutations in Norrin are associated with Norrie disease and X-linked familial exudative vitroretinopathy. The amino acid sequence of human Norrin is 100% and 94% identical to rhesus macaque and mouse Norrin, respectively.
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Western Blot
Cited:
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 343711
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Product Specifications
Immunogen
E. coli-derived recombinant human Norrin
Lys25-Ser133
Accession # Q00604
Lys25-Ser133
Accession # Q00604
Specificity
Detects human and mouse Norrin in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross-reactivity with recombinant mouse Norrin is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for Norrin Antibody (343711)
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Human Norrin (Catalog # 3014-NR) under reducing conditions only
Recombinant Mouse Norrin (Catalog # 3497-NR)
Sample: Recombinant Human Norrin (Catalog # 3014-NR) under reducing conditions only
Recombinant Mouse Norrin (Catalog # 3497-NR)
Reviewed Applications
Read 1 review rated 5 using MAB3014 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Norrin
Additional Norrin Products
Product Documents for Norrin Antibody (343711)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Norrin Antibody (343711)
For research use only
Related Research Areas
Citations for Norrin Antibody (343711)
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Customer Reviews for Norrin Antibody (343711) (1)
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Application: Western BlotSample Tested: Purified mouse Ndp proteinSpecies: MouseVerified Customer | Posted 04/07/2021used at 1ug/mL in 5% Blocking Solution (Biorad) overnight at 4C
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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