Human/Mouse Norrin Antibody

Catalog # Availability Size / Price Qty
MAB3014
MAB3014-SP
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews (1)

Human/Mouse Norrin Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse Norrin in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross-reactivity with recombinant mouse Norrin is observed.
Source
Monoclonal Mouse IgG1 Clone # 343711
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Norrin
Lys25-Ser133
Accession # Q00604
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Norrin (Catalog # 3014-NR) under reducing conditions only
Recombinant Mouse Norrin (Catalog # 3497-NR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Norrin

Norrin, also known as Norrie Disease Protein, is a secreted protein that has a C-terminal cysteine-knot motif. Although Norrin is unrelated to Wnt family proteins, it is a Frizzled-4-specific ligand that activates the canonical Wnt pathway through Frizzled-4 and LRP5 (LDL receptor-related protein). Mutations in Norrin are associated with Norrie disease and X-linked familial exudative vitroretinopathy. The amino acid sequence of human Norrin is 100% and 94% identical to rhesus macaque and mouse Norrin, respectively.

Entrez Gene IDs
4693 (Human)
Alternate Names
EVR2; exudative vitreoretinopathy 2 (X-linked); FEVR; ND; NDP; Norrie disease (pseudoglioma); Norrie disease protein; Norrin; X-linked exudative vitreoretinopathy 2 protein

Product Datasheets

Citation for Human/Mouse Norrin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Proteomics analysis identifies new markers associated with capillary cerebral amyloid angiopathy in Alzheimer's disease
    Authors: DC Hondius, KN Eigenhuis, THJ Morrema, RC van der Sc, P van Nierop, M Bugiani, KW Li, JJM Hoozemans, AB Smit, AJM Rozemuller
    Acta Neuropathol Commun, 2018;6(1):46.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human/Mouse Norrin Antibody

Average Rating: 5 (Based on 1 Review)

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Human/Mouse Norrin Antibody
By Anonymous on 04/07/2021
Application: WB Sample Tested: Purified mouse Ndp protein Species: Mouse

used at 1ug/mL in 5% Blocking Solution (Biorad) overnight at 4C