Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 540421
View all PKN1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PKN1
Ile215-Thr388
Accession # Q16512

Specificity

Detects human and mouse PKN1 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human/Mouse PKN1 Antibody

Detection of Human and Mouse PKN1 antibody by Western Blot.

Detection of Human and Mouse PKN1 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and L1.2 mouse pro-B cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse PKN1 Monoclonal Antibody (Catalog # MAB6100) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PKN1 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse PKN1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and L1.2 mouse pro-B cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PKN1

PKN1 (serine/threonine protein kinase N1), also known as PKN or PRK, is a ubiquitous 120 kDa cytoplasmic kinase of the PKC superfamily. The 942 amino acid (aa) PKN1 contains three HR1 repeats (aa 34‑263), a C2 domain (aa 325‑461), and a protein kinase domain (aa 615‑874). PKN1 can be activated by proteolysis, lipid interaction, or Rho G proteins, and is a downstream effector of RhoA. It promotes the activation of several kinases involved in regulation of the actin cytoskeleton, cell cycle, and apoptosis. In vivo, PKN1 phosphorylates histone H3 at the Thr11 position.

Long Name

Serine/Threonine Protein Kinase N1

Alternate Names

DBK, PAK1, PKN, PKN-ALPHA, PRK1, PRKCL1

Entrez Gene IDs

5585 (Human); 320795 (Mouse); 29355 (Rat)

Gene Symbol

PKN1

UniProt

Q16512 (Human)

Additional PKN1 Products

Product Documents for Human/Mouse PKN1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse PKN1 Antibody

For research use only

Related Research Areas

Diabetes
Epigenetics
Intracellular Kinases

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