Human/Mouse PKN1 Antibody

  
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse PKN1 in Western blots.
  • Source
    Monoclonal Mouse IgG1 Clone # 540421
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human PKN1
    Ile215-Thr388
    Accession # Q16512
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human and Mouse PKN1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and L1.2 mouse pro-B cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse PKN1 Monoclonal Antibody (Catalog # MAB6100) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PKN1 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PKN1

PKN1 (serine/threonine protein kinase N1), also known as PKN or PRK, is a ubiquitous 120 kDa cytoplasmic kinase of the PKC superfamily. The 942 amino acid (aa) PKN1 contains three HR1 repeats (aa 34‑263), a C2 domain (aa 325‑461), and a protein kinase domain (aa 615‑874). PKN1 can be activated by proteolysis, lipid interaction, or Rho G proteins, and is a downstream effector of RhoA. It promotes the activation of several kinases involved in regulation of the actin cytoskeleton, cell cycle, and apoptosis. In vivo, PKN1 phosphorylates histone H3 at the Thr11 position.

  • Long Name:
    Serine/Threonine Protein Kinase N1
  • Entrez Gene IDs:
    5585 (Human); 320795 (Mouse); 29355 (Rat)
  • Alternate Names:
    DBK; EC 2.7.11; MGC46204; PAK1; PAK1Serine-threonine protein kinase N; PKN; PKN1; PKN-ALPHA; PKNPKN-ALPHA; PRK1; PRK1PAK-1; PRKCL1; PRKCL1protein kinase C-related kinase 1; Protease-activated kinase 1; Protein kinase C-like 1EC 2.7.11.13; Protein kinase C-like PKN; protein kinase N1; Protein kinase PKN-alpha; Protein-kinase C-related kinase 1; serine/threonine protein kinase N; serine/threonine-protein kinase N1; serine-threonine kinase N
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Isotype Control

Ctrl MAB002 138  
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Mouse IgG1 Isotype Control

Ctrl MAB002R 1
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse IgG Antibody

WB AF007 4
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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