Detection of Human and Mouse PKN1 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and L1.2 mouse pro-B cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse PKN1 Monoclonal Antibody (Catalog # MAB6100) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PKN1 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
PKN1 (serine/threonine protein kinase N1), also known as PKN or PRK, is a ubiquitous 120 kDa cytoplasmic kinase of the PKC superfamily. The 942 amino acid (aa) PKN1 contains three HR1 repeats (aa 34‑263), a C2 domain (aa 325‑461), and a protein kinase domain (aa 615‑874). PKN1 can be activated by proteolysis, lipid interaction, or Rho G proteins, and is a downstream effector of RhoA. It promotes the activation of several kinases involved in regulation of the actin cytoskeleton, cell cycle, and apoptosis. In vivo, PKN1 phosphorylates histone H3 at the Thr11 position.
Serine/Threonine Protein Kinase N1
Entrez Gene IDs:
5585 (Human); 320795 (Mouse); 29355 (Rat)
DBK; EC 2.7.11; MGC46204; PAK1; PAK1Serine-threonine protein kinase N; PKN; PKN1; PKN-ALPHA; PKNPKN-ALPHA; PRK1; PRK1PAK-1; PRKCL1; PRKCL1protein kinase C-related kinase 1; Protease-activated kinase 1; Protein kinase C-like 1EC 184.108.40.206; Protein kinase C-like PKN; protein kinase N1; Protein kinase PKN-alpha; Protein-kinase C-related kinase 1; serine/threonine protein kinase N; serine/threonine-protein kinase N1; serine-threonine kinase N
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