Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 346404
View all PLC-gamma 2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PLC-gamma 2
Pro400-Tyr600
Accession # P16885

Specificity

Detects human and mouse PLC-gamma 2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human/Mouse PLC‑ gamma 2 Antibody

Detection of Human/Mouse PLC-?2 antibody by Western Blot.

Detection of Human/Mouse PLC-gamma 2 by Western Blot.

Western blot shows lysates of A20 mouse B cell lymphoma cell line and U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse PLC- gamma2 Monoclonal Antibody (Catalog # MAB3716) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PLC- gamma2 at approximately 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
PLC-?2 antibody in Human PBMCs by Immunocytochemistry (ICC).

PLC-gamma 2 in Human PBMCs.

PLC- gamma2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Human/Mouse PLC- gamma2 Monoclonal Antibody (Catalog # MAB3716) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human/Mouse PLC‑ gamma 2 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells

Western Blot

1 µg/mL
Sample: A20 mouse B cell lymphoma cell line and U937 human histiocytic lymphoma cell line

Reviewed Applications

Read 1 review rated 5 using MAB3716 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PLC-gamma 2

The Phospholipase C family consists of 13 isozymes within six subfamilies, PLC-delta, -beta, -gamma, -epsilon, -zeta, and -eta. PLC-gamma 2 (Phospholipase C gamma-2) contains 2 SH2 and 1 SH3 domains and is primarily limited to cells of hematopoietic lineage. PLC-  gamma 2 is activated by receptor tyrosine kinases in response to growth factors, neurotransmitters, and hormones. Activated PLC-  gamma 2 catalyzes the hydrolysis of phosphatidylinositol 4, 5-biphosphate to produce the second messengers inositol 1, 4, 5-triphosphate (IP3) and diacylglycerol (DAG). IP3 mobilizes the release of calcium while DAG activates protein kinase C. PLC-  gamma 2 is involved in collagen induced signaling in platelets and antigen-dependent signaling in B-lymphocytes.

Long Name

Phospholipase C gamma 2

Alternate Names

PLCG2, PLCgamma 2

Entrez Gene IDs

5336 (Human); 234779 (Mouse); 29337 (Rat)

Gene Symbol

PLCG2

UniProt

P16885

Additional PLC-gamma 2 Products

Product Documents for Human/Mouse PLC‑ gamma 2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse PLC‑ gamma 2 Antibody

For research use only

Citations for Human/Mouse PLC‑ gamma 2 Antibody

Customer Reviews for Human/Mouse PLC‑ gamma 2 Antibody (1)

5 out of 5
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  • Human/Mouse PLC‑ gamma 2 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Blood mononuclear cells (PBMCs)
    Species: Human
    Verified Customer | Posted 04/22/2016

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Protocols

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