Human/Mouse PLC-gamma 2 Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot View Larger

Detection of Human/Mouse PLC-gamma 2 by Western Blot. Western blot shows lysates of A20 mouse B cell lymphoma cell line and U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse PLC- gamma2 Monoclonal Antibody (Catalog # MAB3716) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PLC- gamma2 at approximately 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry View Larger

PLC-gamma 2 in Human PBMCs. PLC- gamma2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Human/Mouse PLC- gamma2 Monoclonal Antibody (Catalog # MAB3716) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Preparation and Storage

Background: PLC-gamma 2

The Phospholipase C family consists of 13 isozymes within six subfamilies, PLC-δ, -beta, -gamma, -epsilon, -zeta, and -eta. PLC-gamma 2 (Phospholipase C gamma-2) contains 2 SH2 and 1 SH3 domains and is primarily limited to cells of hematopoietic lineage. PLC-  gamma 2 is activated by receptor tyrosine kinases in response to growth factors, neurotransmitters, and hormones. Activated PLC-  gamma 2 catalyzes the hydrolysis of phosphatidylinositol 4, 5-biphosphate to produce the second messengers inositol 1, 4, 5-triphosphate (IP3) and diacylglycerol (DAG). IP3 mobilizes the release of calcium while DAG activates protein kinase C. PLC-  gamma 2 is involved in collagen induced signaling in platelets and antigen-dependent signaling in B-lymphocytes.

Product Datasheets

Related Research Areas

• Additional Signal Transduction Molecules
• Bioactive Lipids and Receptors
• C-type Lectin Receptors
• GDNF Family
• Lipid/Cholesterol Metabolism Enzymes
• Neurotrophin/Trk Family
• PDGF Family
• Phospholipases, Small GTPases, and Other Molecules in the Akt Pathway
• Platelet Signaling
• Steroid Hormones and Bioactive Lipids
• VEGF Family