Human/Mouse PLC-gamma 2 Antibody

Catalog # Availability Size / Price Qty
Detection of Human/Mouse PLC-gamma 2 by Western Blot.
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Human/Mouse PLC-gamma 2 Antibody Summary

Species Reactivity
Human, Mouse
Detects human and mouse PLC-gamma 2.
Monoclonal Mouse IgG1 Clone # 346404
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human PLC-gamma 2
Accession # P16885
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse PLC-?2 antibody by Western Blot. View Larger

Detection of Human/Mouse PLC-gamma 2 by Western Blot. Western blot shows lysates of A20 mouse B cell lymphoma cell line and U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse PLC- gamma2 Monoclonal Antibody (Catalog # MAB3716) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PLC- gamma2 at approximately 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry PLC-?2 antibody in Human PBMCs by Immunocytochemistry (ICC). View Larger

PLC-gamma 2 in Human PBMCs. PLC- gamma2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Human/Mouse PLC- gamma2 Monoclonal Antibody (Catalog # MAB3716) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PLC-gamma 2

The Phospholipase C family consists of 13 isozymes within six subfamilies, PLC-δ, -beta, -gamma, -epsilon, -zeta, and -eta. PLC-gamma 2 (Phospholipase C gamma-2) contains 2 SH2 and 1 SH3 domains and is primarily limited to cells of hematopoietic lineage. PLC-  gamma 2 is activated by receptor tyrosine kinases in response to growth factors, neurotransmitters, and hormones. Activated PLC-  gamma 2 catalyzes the hydrolysis of phosphatidylinositol 4, 5-biphosphate to produce the second messengers inositol 1, 4, 5-triphosphate (IP3) and diacylglycerol (DAG). IP3 mobilizes the release of calcium while DAG activates protein kinase C. PLC-  gamma 2 is involved in collagen induced signaling in platelets and antigen-dependent signaling in B-lymphocytes.

Long Name
Phospholipase C gamma 2
Entrez Gene IDs
5336 (Human); 234779 (Mouse); 29337 (Rat)
Alternate Names
EC; Phosphoinositide phospholipase C-gamma-2; phospholipase C gamma 2; phospholipase C, gamma 2 (phosphatidylinositol-specific); phospholipase C, gamma 2 (phosphatidylyinositol-specific); Phospholipase C-gamma-2,1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-2; Phospholipase C-IV; PLCG2; PLCgamma 2; PLC-gamma 2; PLC-gamma-2; PLC-IV

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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Human/Mouse PLC‑ gamma 2 Antibody
By Anonymous on 04/22/2016
Application: WB Sample Tested: Blood mononuclear cells (PBMCs) Species: Human