PUM1 Antibody (856332)

R&D Systems | Catalog # MAB7628

R&D Systems

Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 856332
View all PUM1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PUM1
Pro230-Ala372
Accession # Q14671

Specificity

Detects human PUM1 in ELISAs. Detects human and mouse PUM1 in Western Blots

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for PUM1 Antibody (856332)

Detection of Human and Mouse PUM1 antibody by Western Blot.

Detection of Human and Mouse PUM1 by Western Blot.

Western blot shows lysates of SH-SY5Y human neuroblastoma cell line, BG01V human embryonic stem cells, and Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse PUM1 Mono-clonal Antibody (Catalog # MAB7628) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PUM1 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for PUM1 Antibody (856332)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: SH‑SY5Y human neuroblastoma cell line, BG01V human embryonic stem cells, and Neuro‑2A mouse neuroblastoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PUM1

PUM1 (Pumilio homolog 1/Pumilio-1) is a 130-140 kDa member of the PUF (PUmilio and FBF) family of RNA-binding proteins. It is ubiquitously expressed and appears to be involved in cell cycle regulation. Among other things, PUM1 is known to bind to the 3' UTR of p27 mRNA following cell activation. This results in a conformational change in the p27 transcript, allowing for miRNA binding and translational repression, followed by a decline in p27 availability and entry into S-phase of the cell cycle. Human PUM1 is 1186 amino acids (aa) in length. It contains N-terminal Ala-, Gln- and Ser-rich regions (aa 393-815), one PUM-HD region (aa 828-1168) and eight consecutive pumilio repeats (aa 726-1142). There are six utilized Ser/Thr phosphorylation sites and at least three isoform variants. One contains a five aa insert after Gly418, a second possesses an alternative start site 36 aa upstream of the standard site, and a third shows a deletion of aa 597-623 coupled to a two aa insertion after Gln950. Over aa 230-372, human PUM1 is identical to mouse PUM1 in amino acid sequence. Overall, human and mouse PUM1 share 99% aa sequence identity.

Long Name

Pumilio 1

Alternate Names

HSPUM, PUMH1, Pumilio-1, PUML1

Entrez Gene IDs

9698 (Human); 80912 (Mouse); 362609 (Rat)

Gene Symbol

PUM1

UniProt

Q14671 (Human)

Additional PUM1 Products

Product Documents for PUM1 Antibody (856332)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for PUM1 Antibody (856332)

For research use only

Related Research Areas

Translation Regulation

Citations for PUM1 Antibody (856332)

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