14-3-3 epsilon Antibody

R&D Systems | Catalog # AF4419

R&D Systems

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all 14-3-3 epsilon Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human 14-3-3 epsilon
Met1-Gln255
Accession # P62258

Specificity

Detects human, mouse, and rat 14-3-3 epsilon in Western blots. In Western blots, less than 1% cross-reactivity with other recombinant human 14-3-3 isoforms is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for 14-3-3 epsilon Antibody

Detection of Human/Mouse/ Rat 14-3-3e antibody by Western Blot.

Detection of Human/Mouse/ Rat 14‑3‑3 epsilon by Western Blot.

Western blot shows lysates of Raji human Burkitt's lymphoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromo­cytoma cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/ Mouse/Rat 14-3-3e Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4419) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human 14-3-3 beta, epsilon, eta, gamma, sigma, theta, and zeta (2 ng/lane) were included. Specific bands for 14-3-3 epsilon was detected at approximately 26 and 29 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for 14-3-3 epsilon Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Raji human Burkitt's lymphoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromocytoma cell line

Reviewed Applications

Read 1 review rated 3 using AF4419 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: 14-3-3 epsilon

14-3-3 proteins are a highly conserved family of homo- and heterodimeric phosphoserine/threonine-binding proteins present in all eukaryotic cells. 14-3-3 proteins act as key regulators of intracellular signal transduction through their ability to bind specific motifs phosphorylated on serine or threonine. There are at least 7 distinct mammalian 14-3-3 genes: alpha / beta, epsilon, eta, gamma, tau / theta, sigma, and zeta / delta.

Alternate Names

1433 epsilon, YWHAE

Entrez Gene IDs

7531 (Human); 22627 (Mouse); 29753 (Rat)

Gene Symbol

YWHAE

UniProt

P62258

Additional 14-3-3 epsilon Products

Product Documents for 14-3-3 epsilon Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for 14-3-3 epsilon Antibody

For research use only

Customer Reviews for 14-3-3 epsilon Antibody (1)

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  • Human/Mouse/Rat 14-3-3 epsilon Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Myocardium
    Species: Human
    Verified Customer | Posted 01/05/2021
    14-3-3 epsilon Antibody AF4419

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