Detection of Human/Mouse/ Rat 14‑3‑3 eta by Western Blot.
Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Balb/3T3 mouse embryonic fibroblast cell line, and TS1 mouse helper T cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/ Mouse/Rat 14‑3‑3 eta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4420) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, recombinant human 14-3-3 beta, epsilon, eta, gamma, sigma, theta, and zeta (2 ng/lane) were included. A specific band for 14‑3‑3 eta was detected at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: 14-3-3 eta
14-3-3 proteins are a highly conserved family of homo- and heterodimeric phosphoserine/threonine-binding proteins present in all eukaryotic cells. 14-3-3 proteins act as key regulators of intracellular signal transduction through their ability to bind specific motifs phosphorylated on serine or threonine. There are at least 7 distinct mammalian 14-3-3 genes: alpha / beta, epsilon, eta, gamma, tau / theta, sigma, and zeta /δ.
Entrez Gene IDs:
7533 (Human); 22629 (Mouse); 25576 (Rat)
1433 eta; 14-3-3 eta; 14-3-3 protein eta; Protein AS1; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, etapolypeptide; YWHA1; YWHA114-3-3 eta; YWHAH
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