Human/Mouse/Rat 14-3-3 eta Antibody Summary
Accession # Q04917
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human/Mouse/ Rat 14‑3‑3 eta by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Balb/3T3 mouse embryonic fibroblast cell line, and TS1 mouse helper T cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/ Mouse/Rat 14-3-3 eta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4420) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, recombinant human 14-3-3 beta, epsilon, eta, gamma, sigma, theta, and zeta (2 ng/lane) were included. A specific band for 14-3-3 eta was detected at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: 14-3-3 eta
14-3-3 proteins are a highly conserved family of homo- and heterodimeric phosphoserine/threonine-binding proteins present in all eukaryotic cells. 14-3-3 proteins act as key regulators of intracellular signal transduction through their ability to bind specific motifs phosphorylated on serine or threonine. There are at least 7 distinct mammalian 14-3-3 genes: alpha / beta, epsilon, eta, gamma, tau / theta, sigma, and zeta /δ.
Citation for Human/Mouse/Rat 14-3-3 eta Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
YWHA (14-3-3) protein isoforms and their interactions with CDC25B phosphatase in mouse oogenesis and oocyte maturation
Authors: AA Eisa, S De, A Detwiler, E Gilker, AC Ignatious, S Vijayaragh, D Kline
BMC Dev. Biol., 2019;19(1):20.
Sample Types: Tissue Homogenates
Applications: Western Blot
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Reviews for Human/Mouse/Rat 14-3-3 eta Antibody
There are currently no reviews for this product. Be the first to review Human/Mouse/Rat 14-3-3 eta Antibody and earn rewards!
Have you used Human/Mouse/Rat 14-3-3 eta Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image