Human/Mouse/Rat 14-3-3 theta Antibody

Catalog # Availability Size / Price Qty
Detection of Human/Mouse/ Rat 14‑3‑3 theta  by Western Blot.
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Product Details
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Human/Mouse/Rat 14-3-3 theta Antibody Summary

Species Reactivity
Human, Mouse, Rat
Detects human, mouse, and rat 14-3-3 theta in Western blots. Does not cross-react with other recombinant human 14-3-3 isoforms.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant human 14-3-3 theta
Accession # P27348
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse/ Rat 14-3-3? antibody by Western Blot. View Larger

Detection of Human/Mouse/ Rat 14‑3‑3 theta by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromo-cytoma cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/ Mouse/Rat 14-3-3 theta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4425) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human 14-3-3 beta, epsilon, eta, gamma, sigma, theta, and zeta (2 ng/lane) were included. A specific band for 14-3-3 theta was detected at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: 14-3-3 theta

14-3-3 proteins are a highly conserved family of homo- and heterodimeric phosphoserine/threonine-binding proteins present in all eukaryotic cells. 14-3-3 proteins act as key regulators of intracellular signal transduction through their ability to bind specific motifs phosphorylated on serine or threonine. There are at least 7 distinct mammalian 14-3-3 genes: alpha / beta, epsilon, eta, gamma, tau / theta, sigma, and zeta /δ.

Entrez Gene IDs
10971 (Human); 22630 (Mouse); 25577 (Rat)
Alternate Names
14-3-3 protein tau; 14-3-3 protein T-cell; 14-3-3 protein theta; 1433 theta; 14-3-3 theta; 14-3-3,1C5; HS1 protein; HS1; Protein HS1; protein tau; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, thetapolypeptide; YWHAQ

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Goat IgG (H+L) Antibody


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