Human/Mouse/Rat Akt1 Antibody

Name: Human/Mouse/Rat Akt1 Antibody
Brand: R&D Systems
SKU: AF17751
Price: 399 USD
Availability: InStock

R&D Systems | Catalog # AF17751

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all Akt1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Akt1
Met1-Glu149
Accession # P31749

Specificity

Detects human, mouse, and rat Akt1 in Western blots. The antibody is specific for Akt1 and does not detect recombinant Akt2 or Akt3.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat Akt1 Antibody

Detection of Human/Mouse/Rat Akt1 by Western Blot.

Western blot shows lysates of C2C12 mouse myoblast cell line and HL-60 human promyelocytic leukemia cell line, and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL Sheep Anti-Human/Mouse/Rat Akt1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF17751) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). For additional reference, Recombinant Human Active Akt1 (Catalog # 1775-KS), recombinant human Akt2, and recombinant human Akt3 (2 ng/lane) were included. A specific band for Akt1 was detected at approximately 63 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat Akt1 Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: C2C12 mouse myoblast cell line and HL-60 human promyelocytic leukemia cell line, and Raji human Burkitt's lymphoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Akt1

The serine/threonine kinase Akt, also known as protein kinase B (PKB), is a central regulator of such diverse cellular processes as glucose uptake, cell cycle progression, and apoptosis. In mammals, three highly homologous members define the Akt family: Akt1 (PKB alpha ), Akt2 (PKB beta ), and Akt3 (PKB gamma ). Akt1 is the most ubiquitously expressed family member. All three Akts contain an amino-terminal pleckstrin homology domain, a central kinase domain, and a carboxyl-terminal regulatory domain.