Detection of Human/Mouse/Rat AlphaB Crystallin/CRYAB by Western Blot.
Western blot shows lysates of mouse eye lens and human, mouse, and rat heart tissue. PVDF membrane was probed with 0.2 µg/mL Human/Mouse/Rat AlphaB Crystallin/CRYAB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4849) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, recombinant human CRYAA and CRYAB (5 ng/lane) were included. A specific band for AlphaB Crystallin/CRYAB was detected at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Detection of Human and Mouse AlphaB Crystallin/CRYAB by Simple WesternTM.
Simple Western lane view shows lysates of human heart tissue and mouse heart tissue, loaded at 0.2 mg/mL. A specific band was detected for AlphaB Crystallin/CRYAB at approximately 29 kDa (as indicated) using 2 µg/mL of Goat Anti-Human/Mouse/Rat AlphaB Crystallin/CRYAB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4849) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: AlphaB Crystallin/CRYAB
AlphaB Crystallin (CRYAB, also known as Crystallin alpha -B chain and HspB5) is a 22‑23 kDa member of the HSP20 family of proteins. It has widespread expression in many tissues in addition to lens epithelium, where it noncovalently oligomerizes with CRY-alpha A to generate a transparent 350‑1000 kDa alpha -Crystallin protein complex. Human CRYAB is 175 amino acids (aa) in length. There is a alpha -Crystallin Hsp domain over aa 66‑149. Multiple posttranslational modifications may exist. The N‑terminal Met and MetAspIleAlaIleHis sequence is occasionally cleaved. There is also phosphorylation at Ser45 and 59, potential O-GlcNAc modification at Thr158, 162 or 170, and acetylation at Lys92. There is an alternate start site at Met68 that may be accompanied by a 47 aa substitution for aa 109‑175. Full-length human CRYAB is 54% aa identical to CRY-AlphaA, and 98% aa identical to mouse CRYAB.
Entrez Gene IDs:
1410 (Human); 12955 (Mouse); 25420 (Rat)
alpha B crystallin; alpha(B)-crystallin; alpha-crystallin B chain; CRYA2; CRYA2alpha crystallin B chain; CRYAB; crystallin, alpha B; CTPP2; Heat shock protein beta-5; heat-shock 20 kD like-protein; HSPB5; Renal carcinoma antigen NY-REN-27; Rosenthal fiber component
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