Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all AlphaB Crystallin/CRYAB Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human AlphaB Crystallin/CRYAB
Met1-Lys175
Accession # P02511

Specificity

Detects human, mouse and rat AlphaB Crystallin/CRYAB in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human CRYAA is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat AlphaB Crystallin/CRYAB Antibody

Detection of Human/Mouse/Rat AlphaB Crystallin/CRYAB antibody by Western Blot.

Detection of Human/Mouse/Rat AlphaB Crystallin/CRYAB by Western Blot.

Western blot shows lysates of mouse eye lens and human, mouse, and rat heart tissue. PVDF membrane was probed with 0.2 µg/mL Human/Mouse/Rat AlphaB Crystallin/CRYAB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4849) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, recombinant human CRYAA and CRYAB (5 ng/lane) were included. A specific band for AlphaB Crystallin/CRYAB was detected at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Detection of Human and Mouse AlphaB Crystallin/CRYAB antibody by Simple WesternTM.

Detection of Human and Mouse AlphaB Crystallin/CRYAB by Simple WesternTM.

Simple Western lane view shows lysates of human heart tissue and mouse heart tissue, loaded at 0.2 mg/mL. A specific band was detected for AlphaB Crystallin/CRYAB at approximately 29 kDa (as indicated) using 2 µg/mL of Goat Anti-Human/Mouse/Rat AlphaB Crystallin/CRYAB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4849) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human/Mouse/Rat AlphaB Crystallin/CRYAB Antibody

Application
Recommended Usage

Simple Western

2 µg/mL
Sample: Human heart tissue and mouse heart tissue

Western Blot

0.2 µg/mL
Sample: Mouse eye lens and human, mouse, and rat heart tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: AlphaB Crystallin/CRYAB

AlphaB Crystallin (CRYAB, also known as Crystallin alpha -B chain and HspB5) is a 22‑23 kDa member of the HSP20 family of proteins. It has widespread expression in many tissues in addition to lens epithelium, where it noncovalently oligomerizes with CRY-alpha A to generate a transparent 350‑1000 kDa alpha -Crystallin protein complex. Human CRYAB is 175 amino acids (aa) in length. There is a alpha -Crystallin Hsp domain over aa 66‑149. Multiple posttranslational modifications may exist. The
N‑terminal Met and MetAspIleAlaIleHis sequence is occasionally cleaved. There is also phosphorylation at Ser45 and 59, potential O-GlcNAc modification at Thr158, 162 or 170, and acetylation at Lys92. There is an alternate start site at Met68 that may be accompanied by a 47 aa substitution for aa 109‑175. Full-length human CRYAB is 54% aa identical to CRY-AlphaA, and 98% aa identical to mouse CRYAB.

Alternate Names

CRYA2, CRYAB, CTPP2, HSPB5

Entrez Gene IDs

1410 (Human); 12955 (Mouse); 25420 (Rat)

Gene Symbol

CRYAB

UniProt

P02511 (Human)

Additional AlphaB Crystallin/CRYAB Products

Product Documents for Human/Mouse/Rat AlphaB Crystallin/CRYAB Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat AlphaB Crystallin/CRYAB Antibody

For research use only

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