|Detection of Human/Mouse/Rat Annexin A11 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, A20 mouse B cell lymphoma cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Annexin A11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3927) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Annexin A11 at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.|
Annexin A11 (ANXA11), also known as Annexin XI, 56K Autoantigen and Calcyclin-associated annexin 50 (CAP50), is a 54.4 kDa (predicted) member of the Annexin protein family. The Annexins are a family of Calcium-dependent phospholipid-binding proteins that are preferentially located on the cytosolic face of the plasma membrane. The Annexin’s have a molecular weight of approximately 35‑40 kDa and consist of a unique amino terminal domain followed by a homologous C-terminal core domain containing the calcium-dependent phospholipid-binding sites. The C‑terminal domain is comprised of four 60‑70 amino acid (aa) repeats, known as annexin repeats or an endonexin fold (Annexin A6 contains 8 annexin repeats). The four annexin repeats form a highly alpha -helical, tightly packed disc known as the annexin domain, which binds to phospholipids in the membrane in a calcium-dependent manner. Members of the annexin family play a role in cytoskeletal interactions, phospholipase inhibition, regulation of cellular growth, and intracellular signal transduction pathways. Annexin A11 was identified as a 56 kDa antigen recognized by sera from patients with autoimmune diseases such as rheumatoid arthritis, systemic lupus erythematosus, or Sjogren's syndrome. Human Annexin A11 shares 93% identity with mouse and rat Annexin A11.