Human/Mouse/Rat ARC Antibody

  
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  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human, mouse, and rat ARC in Western blots.
  • Source
    Polyclonal Rabbit IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    KLH-coupled human ARC synthetic peptide
    CEPEAEAEPEPELE PEPDPEP
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    Immersion fixed paraffin-embedded sections of human brain (hippocampus), heart and skeletal muscle, mouse skeletal muscle, and rat brain
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human/Mouse/Rat ARC by Western Blot.

Western blot shows lysates of rat and mouse skeletal muscle tissue. PVDF membrane was probed with 1 µg/mL Rabbit Anti-Human/Mouse/Rat ARC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF636) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). For additional reference, Recombinant Human ARC Western Blot Standard (Catalog # WBC009) (1 ng) was included. A specific band for ARC was detected at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ARC
ARC [Apoptosis Repressor with CARD (Caspase-recruitment Domain)] is a 29 kDa cytosolic protein containing an N-terminal CARD joined to a C-terminal region rich in proline and glutamic acid. The expression of human ARC is primarily restricted to skeletal muscle and cardiac tissue. ARC inhibits apoptosis induced by Caspase-8.
  • Long Name:
    Apoptosis Repressor with Caspase Recruitment Domain
  • Entrez Gene IDs:
    8996 (Human); 11838 (Mouse)
  • Alternate Names:
    Apoptosis repressor with CARD; ARC; ARCFLJ35304; CARD2; Muscle-enriched cytoplasmic protein; MYC; MYP; NOL3; NOP30; NOPnucleolar protein 3; nucleolar protein 3 (apoptosis repressor with CARD domain); Nucleolar protein of 30 kDa
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