Human/Mouse/Rat ARC Antibody

Catalog #: AF636 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF636
AF636-SP

Request a Quote Bulk Orders

Detection of Human/Mouse/Rat ARC by Western Blot.

1 Image

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat ARC Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat ARC in Western blots.

Source

Polyclonal Rabbit IgG

Purification

Antigen Affinity-purified

Immunogen

KLH-coupled human ARC synthetic peptide
CEPEAEAEPEPELE PEPDPEP

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Complete Your Research

Human Apolipoprotein E/ApoE Antibody

Cat # MAB41441

(1)

Citations (2)

Human Apolipoprotein E/ApoE Antibody

Cat # AF4144

Citations (2)

Recombinant Human ARC Western Blot Standard Protein

Cat # WBC009

Human Apolipoprotein A-I/ApoA1 Alexa Fluor® 700-conjugated Antibody

Cat # FAB36641N

Recombinant Human Apolipoprotein E R2 Protein, CF

Cat # 3520-AR

Citations (2)

Apolipoprotein E3/ApoE3 antibody in Human Brain by Immunohistochemistry (IHC-P).

Human Apolipoprotein E3/ApoE3 Antibody

Cat # MAB41443

Human Apolipoprotein H/ApoH Antibody

Cat # AF5087

Human LRP-1B Antibody

Cat # AF6379

(1)

Human Apolipoprotein E3/ApoE3 Antibody

Cat # MAB41444

Human Apolipoprotein A-I/ApoA1 Alexa Fluor® 488-conjugated Antibody

Cat # FAB36641G

Human Apolipoprotein E3/ApoE3 Antibody

Cat # MAB41445

Recombinant Human Bcl-w Western Blot Standard Protein

Cat # WBC005

Gemcitabine hydrochloride

Cat # 3259

(2)

Citations (14)

Human Apolipoprotein A-I/ApoA1 Antibody

Cat # AF3664

Citations (4)

Human Apolipoprotein E R2 (NP_004622) VersaClone cDNA

Cat # RDC2616

Human L1CAM Antibody

Cat # MAB777

(1)

Citations (5)

Human FAT10 Antibody

Cat # AF8408

Detection of Human, Mouse, and Rat BOK antibody by Western Blot.

Human/Mouse/Rat BOK Antibody

Cat # AF6067

Citations (1)

Recombinant Rat BOK Western Blot Standard Protein

Cat # WBC004

Human Apolipoprotein B/ApoB Antibody

Cat # AF3556

Citations (3)

Complete Your Experiment

Detection of Rabbit IgG Primary Antibody by Western Blot

Rabbit IgG HRP-conjugated Antibody

Cat # HAF008

(4)

Citations (105)

Detection of Rabbit IgG Isotype Control by Flow Cytometry

Normal Rabbit IgG Control

Cat # AB-105-C

(10)

Citations (83)

Reconstitution Buffer 1 (PBS)

Cat # RB01

(3)

Donkey Anti-Rabbit IgG NorthernLights™ NL557-conjugated Antibody

Cat # NL004

(1)

Citations (23)

Detection of Rabbit IgG Primary Antibody by Flow Cytometry

Rabbit IgG PE-conjugated Antibody

Cat # F0110

(2)

Citations (7)

Rabbit IgG APC-conjugated Antibody

Cat # F0111

(3)

Citations (5)

Detection of Rabbit IgG Antibody by Simple Western<sup>TM</sup>.

Rabbit IgG Biotinylated Antibody

Cat # BAF008

(3)

Citations (7)

Donkey Anti-Rabbit IgG NorthernLights™ NL493-conjugated Antibody

Cat # NL006

Citations (8)

Rabbit IgG VisUCyte HRP Polymer Antibody

Cat # VC003

Citations (3)

Detection of FABP1/L-FABP in Human Kidney.

Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

Cat # VC002

Citations (2)

Rabbit IgG Fluorescein-conjugated Antibody

Cat # F0112

Citations (7)

Rabbit IgG Antibody

Cat # AF008

Citations (4)

Normal Rabbit IgG Control

Cat # MAB1050

Citations (1)

Donkey Anti-Rabbit IgG NorthernLights™ NL637-conjugated Antibody

Cat # NL005

Citations (2)

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

Immersion fixed paraffin-embedded sections of human brain (hippocampus), heart and skeletal muscle, mouse skeletal muscle, and rat brain

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human/Mouse/Rat ARC by Western Blot. Western blot shows lysates of rat and mouse skeletal muscle tissue. PVDF membrane was probed with 1 µg/mL Rabbit Anti-Human/Mouse/Rat ARC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF636) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). For additional reference, Recombinant Human ARC Western Blot Standard (Catalog # WBC009) (1 ng) was included. A specific band for ARC was detected at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ARC

ARC [Apoptosis Repressor with CARD (Caspase-recruitment Domain)] is a 29 kDa cytosolic protein containing an N-terminal CARD joined to a C-terminal region rich in proline and glutamic acid. The expression of human ARC is primarily restricted to skeletal muscle and cardiac tissue. ARC inhibits apoptosis induced by Caspase-8.

Long Name

Apoptosis Repressor with Caspase Recruitment Domain

Entrez Gene IDs

8996 (Human); 11838 (Mouse)

Alternate Names

Apoptosis repressor with CARD; ARC; ARCFLJ35304; CARD2; Muscle-enriched cytoplasmic protein; MYC; MYP; NOL3; NOP30; NOPnucleolar protein 3; nucleolar protein 3 (apoptosis repressor with CARD domain); Nucleolar protein of 30 kDa