Detection of Human/Mouse/Rat Blk by Western Blot.
Western blot shows lysates of A20 mouse B cell lymphoma cell line and CH-1 mouse B cell lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat Blk Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2679) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Blk at approximately 55 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
B-cell lymphocyte kinase (Blk) is a member of the Src family of non‑receptor tyrosine kinases. Constitutively active Blk induces malignant transformation of B-cell progenitors, suggesting a role for Blk in B-lineage cell differentiation and proliferation.
B Lymphoid Tyrosine Kinase
Entrez Gene IDs:
640 (Human); 12124 (Mouse); 364403 (Rat)
B lymphocyte kinase; B lymphoid tyrosine kinase; BLK nonreceptor tyrosine kinase; Blk; EC 184.108.40.206; MGC10442; MODY11; p55-Blk; tyrosine-protein kinase Blk
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