Detects endogenous human, mouse, and rat CK1 alpha in Western blots. In Western blots, this antibody does not cross-react with recombinant human CK1δ or CK1 gamma ; reactivity with CK1 epsilon is unknown.
Polyclonal Sheep IgG
E. coli-derived recombinant human CK1 alpha Ala255-Phe365 Accession # NP_001020276
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Detection of Human and Rat Casein Kinase 1 alpha by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line, NRK rat normal kidney cell line, and Y3‑Ag rat myeloid cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Casein Kinase 1 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4569) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Casein Kinase 1 alpha at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Casein Kinase 1 alpha
Casein Kinase 1 (CK1) represents a group of serine/threonine protein kinases that are present in all eukaryotic organisms. Human CK1 isoforms ( alpha, gamma 1, gamma 2, gamma 3, δ, and epsilon ) act as monomeric constitutively active enzymes that phosphorylate key regulatory proteins involved in the control of cell differentiation, proliferation, chromosome segregation, and circadian rhythms. CK1 family members share a highly conserved kinase domain but differ in their variable N- and C-terminal domains. CK1 alpha is speculated to play a role in mammalian cell cycle progression, spindle dynamics, and chromosome segregation.
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The data collected includes not only links to publications in PubMed,
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