Casein Kinase 1 alpha Antibody

R&D Systems | Catalog # AF4569

R&D Systems

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Casein Kinase 1 alpha Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CK1 alpha
Ala255-Phe365
Accession # NP_001020276

Specificity

Detects endogenous human, mouse, and rat CK1 alpha in Western blots. In Western blots, this antibody does not cross-react with recombinant human CK1 delta or CK1 gamma ; reactivity with CK1 epsilon  is unknown.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Casein Kinase 1 alpha Antibody

Detection of Human and Rat Casein Kinase 1a antibody by Western Blot.

Detection of Human and Rat Casein Kinase 1 alpha by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line, NRK rat normal kidney cell line, and Y3-Ag rat myeloid cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Casein Kinase 1a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4569) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for Casein Kinase 1a at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western Blot Shows Human Casein Kinase 1 alpha Specificity Using Knockdown Cell Line.

Western Blot Shows Human Casein Kinase 1 alpha Specificity Using Knockdown Cell Line.

Western blot shows lysates of HCT 116 human colorectal carcinoma cell line and Casein Kinase 1 alpha knockdown HCT 116 cell line (KD). Nitrocellulose membrane was probed with 2 µg/mL of Human/Mouse/Rat Casein Kinase 1 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4569) followed by HRP-conjugated donkey anti-sheep IgG Secondary Antibody. A specific band was detected for Casein Kinase 1 alpha at approximately 36 kDa (as indicated) in the parental HCT 116 cell line, but is significantly reduced in knockdown HCT 116 cell line. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.
Detection of Casein Kinase 1 alpha by Immunoprecipitation.

Detection of Casein Kinase 1 alpha by Immunoprecipitation.

HCT 116 lysates were prepared and immunoprecipitation was performed using 2.0 µg of Human/Mouse/Rat Casein Kinase 1 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4569) pre-coupled to Dynabeads protein G. Immunoprecipitated Casein Kinase 1 alpha was detected with a Rabbit Anti-Casein Kinase 1 alpha antibody. The Ponceau stained transfer of the blot is shown. SM=4% starting material; UB=4% unbound fraction; IP=immunoprecipitate; HC=antibody heavy chain. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).
Casein Kinase 1 alpha Specificity is Shown by Immunocytochemistry in Knockdown Cell Line.

Casein Kinase 1 alpha Specificity is Shown by Immunocytochemistry in Knockdown Cell Line.

HCT 116 WT and Casein Kinase 1 alpha KD cells were labelled with a green or a far-red fluorescent dye, respectively. Cells were stained with Human/Mouse/Rat Casein Kinase 1 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4569) followed by incubation with a donkey anti-sheep Alexa-fluor 555 coupled secondary antibody (upper panel). DAPI-only counterstained cells shown on a lower panel. Acquisition of the blue (nucleus-DAPI), green (identification of WT cells), red (antibody staining) and far-red (identification of KD cells) channels was performed. Representative images of the blue and red (grayscale) channels are shown. WT and KD cells are outlined with green and magenta dashed line, respectively. Primary antibody concentration used: 2 µg/mL. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).

Applications for Casein Kinase 1 alpha Antibody

Application
Recommended Usage

Immunocytochemistry

2 µg/mL
Sample: HCT 116 human colorectal carcinoma cell line

Immunoprecipitation

2 µg/1 mg cell lysate
Sample: Lysates of HCT 116 human colorectal carcinoma cell line

Western Blot

1 µg/mL
Sample: NRK rat normal kidney cell, HeLa human cervical epithelial carcinoma cell line,K562 human chronic myelogenous leukemia cell line, Y3-Ag rat myeloid cell line, and HCT 116 human colorectal carcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Casein Kinase 1 alpha

Casein Kinase 1 (CK1) represents a group of serine/threonine protein kinases that are present in all eukaryotic organisms. Human CK1 isoforms ( alpha, gamma 1, gamma 2, gamma 3, delta, and  epsilon ) act as monomeric constitutively active enzymes that phosphorylate key regulatory proteins involved in the control of cell differentiation, proliferation, chromosome segregation, and circadian rhythms. CK1 family members share a highly conserved kinase domain but differ in their variable N- and C-terminal domains. CK1 alpha is speculated to play a role in mammalian cell cycle progression, spindle dynamics, and chromosome segregation.

Alternate Names

CSNK1A1, HLCDGP1

Entrez Gene IDs

1452 (Human); 93687 (Mouse); 113927 (Rat)

Gene Symbol

CSNK1A1

UniProt

NP_001020276

Additional Casein Kinase 1 alpha Products

Product Documents for Casein Kinase 1 alpha Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Casein Kinase 1 alpha Antibody

For research use only

Related Research Areas

Intracellular Kinases

Citations for Casein Kinase 1 alpha Antibody

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Protocols

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