|Detection of Human and Mouse CDC14 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Raji human Burkitt's lymphoma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat CDC14 Monoclonal Antibody (Catalog # MAB4457) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CDC14 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
Cell Division Cycle 14 (CDC14) is a dual tyrosine and serine/threonine phosphatase. There are two CDC14 proteins, CDC14A with a molecular weight of 70 kDa, and CDC14B with a molecular weight of 53 kDa. CDC14A is primarily associated with the nucleolus, bound to Cfi1/Net1 during cell cycle interphase. During anaphase and telophase, CDC14 redistributes to the nucleoplasm and cytoplasm, where it plays a role in segregating and separating sister chromatids. Mutations that alter CDC14 function cause uneven distribution of chromosomes in dividing cells.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.
The document you requested is not available online. Please enter the Catalog Number and Lot Number below to have a document emailed to you at the address provided