Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 472508
View all CDC14 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CDC14A
Met15-Gly366
Accession # Q9UNH5

Specificity

Detects endogenous human, mouse and rat CDC14A in Western blots. In Western blots, this antibody does not cross‑react with recombinant human PTEN. Reactivity with CDC14B is not known.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human/Mouse/Rat CDC14 Antibody

Detection of Human and Mouse CDC14 antibody by Western Blot.

Detection of Human and Mouse CDC14 by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Raji human Burkitt's lymphoma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat CDC14 Monoclonal Antibody (Catalog # MAB4457) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CDC14 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human, Mouse, and Rat CDC14 antibody by Western Blot.

Detection of Human, Mouse, and Rat CDC14 by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, C2C12 mouse myoblast cell line, H4-II-E-C3 rat hepatoma cell line, L6 rat myoblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat CDC14 Monoclonal Antibody (Catalog # MAB4457) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for CDC14 at approximately 50-75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat CDC14 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line, Raji human Burkitt's lymphoma cell line, C2C12 mouse myoblast cell line, H4‑II‑E‑C3 rat hepatoma cell line, L6 rat myoblast cell line, and Rat‑2 rat embryonic fibroblast cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CDC14

Cell Division Cycle 14 (CDC14) is a dual tyrosine and serine/threonine phosphatase. There are two CDC14 proteins, CDC14A with a molecular weight of 70 kDa, and CDC14B with a molecular weight of 53 kDa. CDC14A is primarily associated with the nucleolus, bound to Cfi1/Net1 during cell cycle interphase. During anaphase and telophase, CDC14 redistributes to the nucleoplasm and cytoplasm, where it plays a role in segregating and separating sister chromatids. Mutations that alter CDC14 function cause uneven distribution of chromosomes in dividing cells.

Long Name

Cell Division Cycle 14

Alternate Names

CDC14A

Entrez Gene IDs

8556 (Human); 229776 (Mouse); 310806 (Rat)

Gene Symbol

CDC14A

UniProt

Q9UNH5

Additional CDC14 Products

Product Documents for Human/Mouse/Rat CDC14 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse/Rat CDC14 Antibody

For research use only

Related Research Areas

Cell Cycle
Phosphatases and Regulators

Citations for Human/Mouse/Rat CDC14 Antibody

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