Cell Division Cycle 14 (CDC14) is a dual tyrosine and serine/threonine phosphatase. There are two CDC14 proteins, CDC14A with a molecular weight of 70 kDa, and CDC14B with a molecular weight of 53 kDa. CDC14A is primarily associated with the nucleolus, bound to Cfi1/Net1 during cell cycle interphase. During anaphase and telophase, CDC14 redistributes to the nucleoplasm and cytoplasm, where it plays a role in segregating and separating sister chromatids. Mutations that alter CDC14 function cause uneven distribution of chromosomes in dividing cells.
CDC14 Antibody (472508)
R&D Systems | Catalog # MAB4457
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Mouse
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 472508
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Product Specifications
Immunogen
E. coli-derived recombinant human CDC14A
Met15-Gly366
Accession # Q9UNH5
Met15-Gly366
Accession # Q9UNH5
Specificity
Detects endogenous human, mouse and rat CDC14A in Western blots. In Western blots, this antibody does not cross‑react with recombinant human PTEN. Reactivity with CDC14B is not known.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for CDC14 Antibody (472508)
Detection of Human and Mouse CDC14 by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Raji human Burkitt's lymphoma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat CDC14 Monoclonal Antibody (Catalog # MAB4457) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CDC14 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human, Mouse, and Rat CDC14 by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, C2C12 mouse myoblast cell line, H4-II-E-C3 rat hepatoma cell line, L6 rat myoblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat CDC14 Monoclonal Antibody (Catalog # MAB4457) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for CDC14 at approximately 50-75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for CDC14 Antibody (472508)
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line, Raji human Burkitt's lymphoma cell line, C2C12 mouse myoblast cell line, H4‑II‑E‑C3 rat hepatoma cell line, L6 rat myoblast cell line, and Rat‑2 rat embryonic fibroblast cell line
Sample: HepG2 human hepatocellular carcinoma cell line, Raji human Burkitt's lymphoma cell line, C2C12 mouse myoblast cell line, H4‑II‑E‑C3 rat hepatoma cell line, L6 rat myoblast cell line, and Rat‑2 rat embryonic fibroblast cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: CDC14
Long Name
Cell Division Cycle 14
Alternate Names
CDC14A
Gene Symbol
CDC14A
UniProt
Additional CDC14 Products
Product Documents for CDC14 Antibody (472508)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for CDC14 Antibody (472508)
For research use only
Related Research Areas
Citations for CDC14 Antibody (472508)
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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