Human/Mouse/Rat Chk1 Antibody

(1 citations)   
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human, mouse, and rat Chk1 in Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human Chk1
    Tyr157-Thr476
    Accession # O14757
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human, Mouse, and Rat Chk1 by Western Blot. Western blot shows lysates of NRK rat normal kidney cell line, U2OS human osteosarcoma cell line, MCF‑7 human breast cancer cell line, CEM human T‑lymphoblastoid cell line, and Balb/3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Chk1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1630) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Chk1 at approximately 57 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Chk1
The Chk1 checkpoint kinase is an integral member of a signaling cascade that controls cell cycle progression. In response to genotoxic stress, Chk1 is phosphorylated by ATM or ATM-related kinases. In turn, Chk1 phosphorylates downstream effectors, such as p53 or the Cdc25 phosphatases to halt cell cycle progression and allow time for repair of incurred damage.
  • Long Name:
    Checkpoint Kinase 1
  • Entrez Gene IDs:
    1111 (Human); 12649 (Mouse); 140583 (Rat)
  • Alternate Names:
    Checkpoint, S. pombe, homolog of, 1; CHEK1; CHK1 (checkpoint, S.pombe) homolog; CHK1 checkpoint homolog (S. pombe); CHK1serine/threonine-protein kinase Chk1; EC 2.7.11; EC 2.7.11.1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Showing Results 1 - 1 of 1

  1. VIP blockade leads to microcephaly in mice via disruption of Mcph1-Chk1 signaling.
    Authors: Passemard S, El Ghouzzi V, Nasser H, Verney C, Vodjdani G, Lacaud A, Lebon S, Laburthe M, Robberecht P, Nardelli J, Mani S, Verloes A, Gressens P, Lelievre V
    J. Clin. Invest., 2011;121(0):3071-87.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: Western Blot
ExactaChIP Chromatin Immunoprecipitation Kits
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ExactaChIP Chromatin IP Buffer Panel

ChIP ECP001 2
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Isotype Controls
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Normal Goat IgG Control

Ctrl AB-108-C 127
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Molecular Weight Markers
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Full-Range Molecular Weight Marker, Biotinylated

MW001
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Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG Horseradish Peroxidase-conjugated Antibody

WB, Simple Western HAF109 12
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF017 11
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 2
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 3
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 4
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 4
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 2
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Donkey Anti-Goat IgG Antibody

WB AF109 4
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Goat IgG VisUCyte HRP Polymer Antibody

IHC VC004
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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