Human/Mouse/Rat Chk1 Antibody Summary
Accession # O14757
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human, Mouse, and Rat Chk1 by Western Blot. Western blot shows lysates of NRK rat normal kidney cell line, U2OS human osteosarcoma cell line, MCF-7 human breast cancer cell line, CEM human T-lymphoblastoid cell line, and Balb/3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Chk1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1630) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Chk1 at approximately 57 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
The Chk1 checkpoint kinase is an integral member of a signaling cascade that controls cell cycle progression. In response to genotoxic stress, Chk1 is phosphorylated by ATM or ATM-related kinases. In turn, Chk1 phosphorylates downstream effectors, such as p53 or the Cdc25 phosphatases to halt cell cycle progression and allow time for repair of incurred damage.
Citation for Human/Mouse/Rat Chk1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
VIP blockade leads to microcephaly in mice via disruption of Mcph1-Chk1 signaling.
Authors: Passemard S, El Ghouzzi V, Nasser H, Verney C, Vodjdani G, Lacaud A, Lebon S, Laburthe M, Robberecht P, Nardelli J, Mani S, Verloes A, Gressens P, Lelievre V
J. Clin. Invest., 2011;121(0):3071-87.
Sample Types: Cell Lysates
Applications: Western Blot
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