Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat, Primate, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Simple Western

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry, Mass Spectrometry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Contactin-2/TAG1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Contactin-2/TAG1
Gln31-Ser1014
Accession # Q61330

Specificity

Detects mouse and rat Contactin-2 in direct ELISAs. Detects human, mouse, and rat Contactin-2 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat Contactin‑2/TAG1 Antibody

Detection of Human, Mouse, and Rat Contactin-2/TAG1 antibody by Western Blot.

Detection of Human, Mouse, and Rat Contactin‑2/TAG1 by Western Blot.

Western blot shows lysates of human cerebellum tissue, mouse brain tissue, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Contactin-2/TAG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4439) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Contactin-2/TAG1 at approximately 135 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Contactin-2/TAG1 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr).

Contactin‑2/TAG1 in Mouse Embryo.

Contactin-2/TAG1 was detected in immersion fixed frozen sections of mouse embryo (E13) using Goat Anti-Human/Mouse/Rat Contactin-2/TAG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4439) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to muscle cells in proximity to ribs. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Detection of Mouse Contactin‑2/TAG1 antibody by Simple WesternTM.

Detection of Mouse Contactin‑2/TAG1 by Simple WesternTM.

Simple Western lane view shows lysates of mouse spinal cord tissue, loaded at 0.2 mg/mL. A specific band was detected for Contactin-2/TAG1 at approximately 162 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat Contactin-2/TAG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4439) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of Mouse Human/Mouse/Rat Contactin-2/TAG1 Antibody by Western Blot

Detection of Mouse Human/Mouse/Rat Contactin-2/TAG1 Antibody by Western Blot

Quantitative proteomics in Rab35 cKO P0 hippocampus.a Volcano plot of the TMT-based quantitative proteomes identifying the dysregulated proteins in Rab35 cKO hippocampus in comparison with the control hippocampus (n = 5 mice per genotype). b Number of proteins identified as significantly dysregulated and as either membrane traffic-related or neuronal migration-related. c, d Western blot analysis of control and Rab35 cKO P0 hippocampi using anti-contactin-2, anti-CHL1, and anti-actin antibodies. e, f Quantification of contactin-2 (c) and CHL1 (d) protein levels in control and Rab35 cKO P0 hippocampi. Band intensities of the indicated proteins were normalized to those of actin (n = 9 mice per genotype). Unpaired Student’s t-test; e, p = 0.0153; f, p = 0.0095. g, h Levels of contactin-2 (g) and CHL1 (h) were quantified by targeted MS using the PRM method (n = 5 mice per genotype). Unpaired Student’s t-test; gp = 0.0053; hp = 0.0229. i Western blot analysis of control and Rab35 cKO P0 hippocampus using anti-N-cadherin and anti-actin antibodies. j Quantification of N-cadherin protein levels in the control and Rab35 cKO P0 hippocampus (n = 9 mice per genotypes). Unpaired Student’s t-test, p = 0.9020. k Representative images of DIV 2 hippocampal primary neurons stained for contactin-2 (green), rhodamine-phalloidin (magenta) and DAPI (blue). Scale bar, 20 μm. l Quantification of contactin-2 intensity at the somatic plasma membrane in control (n = 4) and Rab35-deficient (n = 4) cells. Thirty neurons from four different cultures per genotype were analyzed. Mann–Whitney U-test, p = 0.0286. Data represent the mean ± SEM; n.s. not significant (p > 0.05); *p < 0.05; **p < 0.01. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/37085665), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human/Mouse/Rat Contactin‑2/TAG1 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample:

Immersion fixed frozen sections of mouse embryo (E13)

 

Simple Western

10 µg/mL
Sample: Mouse spinal cord tissue

Western Blot

1 µg/mL
Sample: Human cerebellum tissue, Mouse brain tissue, and Rat brain tissue

Reviewed Applications

Read 2 reviews rated 4.5 using AF4439 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Contactin-2/TAG1

Contactin-2 (CNTN2), also called TAG1 (transient axonal glycoprotein), TAX1 (transiently-expressed axonal glycoprotein), or axonin-1, is a 135 kDa glycosyl-phosphatidylinositol (GPI)- anchored cell adhesion molecule that belongs to the contactin subfamily within the immunoglobulin (Ig) protein superfamily (1-3). Mouse Contactin-2 cDNA encodes a 30 amino acid (aa) signal peptide, a 984 aa mature secreted protein with 6 Ig-like domains followed by 4 fibronectin type III-like repeats, and a 26 aa C-terminal GPI anchor pro-sequence. GPI-specific phospholipase activity can release soluble, active Contactin-2 from the membrane (2). Mature mouse Contactin-2 shares approximately 93%, 97%, and 77% aa sequence identity with human, rat, and chicken Contactin-2, respectively. During development, Contactin-2 is expressed by a subset of neuronal populations in the central nervous system (CNS) and peripheral nervous system (PNS), particularly during initial phases of axon outgrowth (3-5). Both the 135 kDa form and a 90 kDa form are also upregulated in response to CNS injury in the adult (6). Data support a role for Contactin-2 in axon pathfinding, neurite outgrowth and adhesion, especially in the CNS (3-6). In mature myelinated fibers, Contactin-2 is expressed by oligodendrocytes and Schwann cells, which are myelinating glial cells of the CNS and PNS, respectively (7, 8). It is enriched in the juxtaparanodal regions, where it recruits contactin-associated protein 2 (caspr2), a transmembrane neurexin involved in cell adhesion and intercellular communication (7-10). The axonal Contactin-2 interacts in cis with caspr2 and in trans with another Contactin-2 on the glial membrane (8). This ternary complex is required for the accumulation and organization of K+ channels in the juxtaparanodes (9).

References

  1. Wolfer, D. and R.J. Giger (1994) Swissprot Accession # Q61330.
  2. Hasler, T.H. et al. (1993) Eur. J. Biochem. 211:329.
  3. Karagogeos, D. (2003) Front. Biosci. 8:s1304.
  4. Liu, Y. and M.C. Halloran (2005) J. Neurosci. 25:10556.
  5. Denaxa, M. et al. (2005) Dev. Biol. 288:87.
  6. Soares, S. et al. (2005) Eur. J. Neurosci. 21:1169.
  7. Traka, M. et al. (2002) J. Neurosci. 22:3016.
  8. Poliak, S. and E. Peles (2003) Nat. Rev. Neurosci. 4:968.
  9. Traka, M. et al. (2003) J. Cell Biol. 162:1161.
  10. Poliak, S. et al. (2003) J. Cell Biol. 162:1149.

Alternate Names

Axonin-1, AXT, CNTN2, Contactin2, TAG1, TAX1

Entrez Gene IDs

6900 (Human); 21367 (Mouse)

Gene Symbol

CNTN2

UniProt

Q61330

Additional Contactin-2/TAG1 Products

Product Documents for Human/Mouse/Rat Contactin‑2/TAG1 Antibody

Certificate of Analysis

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Product Specific Notices for Human/Mouse/Rat Contactin‑2/TAG1 Antibody

For research use only

Related Research Areas

Immunoglobulin Superfamily CAMs

Citations for Human/Mouse/Rat Contactin‑2/TAG1 Antibody

Customer Reviews for Human/Mouse/Rat Contactin‑2/TAG1 Antibody (2)

4.5 out of 5
2 Customer Ratings
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  • Human/Mouse/Rat Contactin-2/TAG1 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: mouse heart
    Species: Mouse
    Verified Customer | Posted 08/05/2020
    Human/Mouse/Rat Contactin‑2/TAG1 Antibody AF4439
  • Mouse/Rat Contactin‑2/TAG1 Antibody
    Name: Rob Andrew
    Application: Western Blot
    Sample Tested: Brain tissue
    Species: Mouse
    Verified Customer | Posted 04/26/2016
    80, 40 and 20ug of total mouse brain homogenate was separated by SDS-PAGE and transfered to PVDF membrane Block: 1% BSA, 1% FSG PBS-T 1h RT Primary: 1:1000, 1% BSA, 1% FSG PBS-T O/N 4oC Secondary: 1:5000,1% BSA, 1% FSG PBS-T 2h RT (Secondary - DaG 800, Licor)
    Human/Mouse/Rat Contactin‑2/TAG1 Antibody AF4439

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