Human/Mouse/Rat Contactin-2/TAG1 Antibody
Human/Mouse/Rat Contactin-2/TAG1 Antibody Summary
Gln31-Ser1014
Accession # Q61330
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human, Mouse, and Rat Contactin‑2/TAG1 by Western Blot. Western blot shows lysates of human cerebellum tissue, mouse brain tissue, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Contactin-2/TAG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4439) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Contactin-2/TAG1 at approximately 135 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Contactin‑2/TAG1 in Mouse Embryo. Contactin-2/TAG1 was detected in immersion fixed frozen sections of mouse embryo (E13) using Goat Anti-Human/Mouse/Rat Contactin-2/TAG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4439) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to muscle cells in proximity to ribs. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
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Detection of Mouse Contactin‑2/TAG1 by Simple WesternTM. Simple Western lane view shows lysates of mouse spinal cord tissue, loaded at 0.2 mg/mL. A specific band was detected for Contactin-2/TAG1 at approximately 162 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat Contactin-2/TAG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4439) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Contactin-2/TAG1
Contactin-2 (CNTN2), also called TAG1 (transient axonal glycoprotein), TAX1 (transiently-expressed axonal glycoprotein), or axonin-1, is a 135 kDa glycosyl-phosphatidylinositol (GPI)- anchored cell adhesion molecule that belongs to the contactin subfamily within the immunoglobulin (Ig) protein superfamily (1-3). Mouse Contactin-2 cDNA encodes a 30 amino acid (aa) signal peptide, a 984 aa mature secreted protein with 6 Ig-like domains followed by 4 fibronectin type III-like repeats, and a 26 aa C-terminal GPI anchor pro-sequence. GPI-specific phospholipase activity can release soluble, active Contactin-2 from the membrane (2). Mature mouse Contactin-2 shares approximately 93%, 97%, and 77% aa sequence identity with human, rat, and chicken Contactin-2, respectively. During development, Contactin-2 is expressed by a subset of neuronal populations in the central nervous system (CNS) and peripheral nervous system (PNS), particularly during initial phases of axon outgrowth (3-5). Both the 135 kDa form and a 90 kDa form are also upregulated in response to CNS injury in the adult (6). Data support a role for Contactin-2 in axon pathfinding, neurite outgrowth and adhesion, especially in the CNS (3-6). In mature myelinated fibers, Contactin-2 is expressed by oligodendrocytes and Schwann cells, which are myelinating glial cells of the CNS and PNS, respectively (7, 8). It is enriched in the juxtaparanodal regions, where it recruits contactin-associated protein 2 (caspr2), a transmembrane neurexin involved in cell adhesion and intercellular communication (7-10). The axonal Contactin-2 interacts in cis with caspr2 and in trans with another Contactin-2 on the glial membrane (8). This ternary complex is required for the accumulation and organization of K+ channels in the juxtaparanodes (9).
- Wolfer, D. and R.J. Giger (1994) Swissprot Accession # Q61330.
- Hasler, T.H. et al. (1993) Eur. J. Biochem. 211:329.
- Karagogeos, D. (2003) Front. Biosci. 8:s1304.
- Liu, Y. and M.C. Halloran (2005) J. Neurosci. 25:10556.
- Denaxa, M. et al. (2005) Dev. Biol. 288:87.
- Soares, S. et al. (2005) Eur. J. Neurosci. 21:1169.
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Traka, M. et al. (2002) J. Neurosci. 22:3016.
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Poliak, S. and E. Peles (2003) Nat. Rev. Neurosci. 4:968.
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Traka, M. et al. (2003) J. Cell Biol. 162:1161.
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Poliak, S. et al. (2003) J. Cell Biol. 162:1149.
Product Datasheets
Citations for Human/Mouse/Rat Contactin-2/TAG1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
13
Citations: Showing 1 - 10
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Histological Analysis of a Mouse Model of the 22q11.2 Microdeletion Syndrome
Authors: Tabata, H;Mori, D;Matsuki, T;Yoshizaki, K;Asai, M;Nakayama, A;Ozaki, N;Nagata, KI;
Biomolecules
Species: Transgenic Mouse
Sample Types: Whole Tissue
Applications: IHC -
Defective Jagged1 signaling impacts GnRH development and contributes to congenital hypogonadotropic hypogonadism
Authors: L Cotellessa, F Marelli, P Duminuco, M Adamo, GE Papadakis, L Bartoloni, N Sato, M Lang-Murit, A Troendle, WS Dhillo, A Morelli, G Guarnieri, N Pitteloud, L Persani, M Bonomi, P Giacobini, V Vezzoli
JCI Insight, 2023-03-08;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Deletion of Sphingosine 1-Phosphate receptor 1 in cardiomyocytes during development leads to abnormal ventricular conduction and fibrosis
Authors: R Jorgensen, M Katta, J Wolfe, DF Leach, B Lavelle, J Chun, LD Wilsbacher
Physiological Reports, 2021-10-01;9(19):e15060.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
NOTCH1 is critical for fibroblast-mediated induction of cardiomyocyte specialization into ventricular conduction system-like cells in vitro
Authors: A Ribeiro da, EA Neri, LT Turaça, R Dariolli, MH Fonseca-Al, A Santos-Mir, D Roman-Camp, G Venturini, JE Krieger
Sci Rep, 2020-09-30;10(1):16163.
Species: Rat
Sample Types: Whole Cells
Applications: ICC -
Afadin Signaling at the Spinal Neuroepithelium Regulates Central Canal Formation and Gait Selection
Authors: S Skarlatou, C Hérent, E Toscano, CS Mendes, J Bouvier, N Zampieri
Cell Rep, 2020-06-09;31(10):107741.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Conditional ablation and conditional rescue models for Casq2 elucidate the role of development and of cell type specific expression of Casq2 in the CPVT2 phenotype
Authors: DJ Flores, T Duong, LO Brandenber, A Mitra, A Shirali, JC Johnson, D Springer, A Noguchi, ZX Yu, SN Ebert, A Ludwig, BC Knollmann, MD Levin, K Pfeifer
Hum. Mol. Genet., 2018-05-01;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
MUNC18-1 gene abnormalities are involved in neurodevelopmental disorders through defective cortical architecture during brain development
Authors: N Hamada, I Iwamoto, H Tabata, KI Nagata
Acta Neuropathol Commun, 2017-11-30;5(1):92.
Species: Primate
Sample Types: Whole Cells
Applications: ICC -
Genome Stability by DNA polymerase ? in Neural Progenitors Contributes to Neuronal Differentiation in Cortical Development
Authors: K Onishi, A Uyeda, M Shida, T Hirayama, T Yagi, N Yamamoto, N Sugo
J. Neurosci., 2017-08-01;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Phenotypically silent Cre recombination within the postnatal ventricular conduction system
Authors: S Bhattachar, M Bhakta, NV Munshi
PLoS ONE, 2017-03-30;12(3):e0174517.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC-Fr, Western Blot -
NOVA2-mediated RNA regulation is required for axonal pathfinding during development
Elife, 2016-05-25;5(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
An aberrant sugar modification of BACE1 blocks its lysosomal targeting in Alzheimer's disease.
Authors: Kizuka Y, Kitazume S, Fujinawa R, Saito T, Iwata N, Saido T, Nakano M, Yamaguchi Y, Hashimoto Y, Staufenbiel M, Hatsuta H, Murayama S, Manya H, Endo T, Taniguchi N
EMBO Mol Med, 2015-02-01;7(2):175-89.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
BACE1 activity regulates cell surface contactin-2 levels.
Authors: Gautam, Vivek, D'Avanzo, Carla, Hebisch, Matthias, Kovacs, Dora M, Kim, Doo Yeon
Mol Neurodegener, 2014-01-09;9(0):4.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Secretome protein enrichment identifies physiological BACE1 protease substrates in neurons.
Authors: Kuhn PH, Koroniak K, Hogl S, Colombo A, Zeitschel U, Willem M, Volbracht C, Schepers U, Imhof A, Hoffmeister A, Haass C, Rossner S, Brase S, Lichtenthaler SF
EMBO J., 2012-06-22;31(14):3157-68.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
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80, 40 and 20ug of total mouse brain homogenate was separated by SDS-PAGE and transfered to PVDF membrane
Block: 1% BSA, 1% FSG PBS-T 1h RT
Primary: 1:1000, 1% BSA, 1% FSG PBS-T O/N 4oC
Secondary: 1:5000,1% BSA, 1% FSG PBS-T 2h RT
(Secondary - DaG 800, Licor)
