Human/Mouse/Rat CrkL Antibody

  
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human, mouse, and rat CrkL in Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human CrkL
    Met1-Glu303
    Accession # P46109
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Simple Western
    1-20 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human, Mouse, and Rat CrkL by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, BaF3 mouse pro-B cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat CrkL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5127) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CrkL at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human CrkL by Simple WesternTM. Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for CrkL at approximately 44 kDa (as indicated) using 1 µg/mL of Goat Anti-Human/Mouse/Rat CrkL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5127) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.
Detection of Human, Mouse and Rat CrkL by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, BaF3 mouse pro-B cell line, and Rat‑2 rat embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for CrkL at approximately 43 and 44 kDa (as indicated) using 20 µg/mL of Goat Anti-Human/Mouse/Rat CrkL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5127) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CrkL

CrkL is a widely-expressed 39 kD adaptor protein that belongs to the Crk protein family and is abundantly expressed in cells of hematopoetic origin. Through its SH2 and SH3 domains CrkL initiates interactions with numerous downstream proteins such as CBL, Cas, Paxillin, C3G, SOS, PI3K, c-Abl and BCR/Abl. It is involved in processes such as adhesion, migration and immune responses. Furthermore, CrkL plays an essential role in Chronic Myelogenous Leukemia and is constitutively phosphorylated by the Bcr-Abl kinase in this disease.

  • Long Name:
    V-Crk Sarcoma Virus CT10 Oncogene Homolog-like Protein
  • Entrez Gene IDs:
    1399 (Human); 12929 (Mouse); 287942 (Rat)
  • Alternate Names:
    CrkL; crk-like protein; v-crk avian sarcoma virus CT10 oncogene homolog-like; v-crk sarcoma virus CT10 oncogene homolog (avian)-like
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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