Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Primate - Chlorocebus aethiops (African Green Monkey)

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all DYRK3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human DYRK3
Asn52-Glu167
Accession # O43781

Specificity

Detects endogenous human, mouse and rat DYRK3 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat DYRK3 Antibody

Detection of Human/Mouse/Rat DYRK3 antibody by Western Blot.

Detection of Human/Mouse/Rat DYRK3 by Western Blot.

Western blot shows lysates of HUVEC human umbilical vein endothelial cells, C2C12 mouse myoblast cell line, L6 rat myoblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat DYRK3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5409) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for DYRK3 at approximately 72 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat DYRK3 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HUVEC human umbilical vein endothelial cells, C2C12 mouse myoblast cell line, L6 rat myoblast cell line, and Rat-2 rat embryonic fibroblast cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DYRK3

DYRK3 (Dual-specificity tyrosine [Y] phosphorylation regulated kinase 3; also REDK) is a member of the MNB/DYRK subfamily, CMGC Ser/Thr protein kinase family of enzymes. DYRK3 is expressed in testis, erythroid-lineage precursors and other tissues, and shows dual substrate specificity. Autophosphorylation on Tyr369 leads to self-activation, while target proteins are Ser/Thr phosphorylated. Targets include CREB and histone 2B. Human DYRK3 is 588 amino acids (aa) in length, and contains one kinase catalytic domain (aa 209‑522). There are two potential isoform variants that show either a six aa substitution for aa 1‑26, or this same substitution in combination with a premature truncation after Gln263.

Long Name

Dual-specificity Tyrosine-[Y]-phosphorylation Regulated Kinase 3

Alternate Names

DYRK5, REDK, YAK3-2

Entrez Gene IDs

8444 (Human); 226419 (Mouse); 304775 (Rat)

Gene Symbol

DYRK3

UniProt

O43781

Additional DYRK3 Products

Product Documents for Human/Mouse/Rat DYRK3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat DYRK3 Antibody

For research use only

Related Research Areas

Intracellular Kinases

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