Human/Mouse/Rat DYRK3 Antibody

  
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects endogenous human, mouse and rat DYRK3 in Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human DYRK3
    Asn52-Glu167
    Accession # O43781
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human/Mouse/Rat DYRK3 by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells, C2C12 mouse myoblast cell line, L6 rat myoblast cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat DYRK3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5409) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for DYRK3 at approximately 72 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DYRK3

DYRK3 (Dual-specificity tyrosine [Y] phosphorylation regulated kinase 3; also REDK) is a member of the MNB/DYRK subfamily, CMGC Ser/Thr protein kinase family of enzymes. DYRK3 is expressed in testis, erythroid-lineage precursors and other tissues, and shows dual substrate specificity. Autophosphorylation on Tyr369 leads to self-activation, while target proteins are Ser/Thr phosphorylated. Targets include CREB and histone 2B. Human DYRK3 is 588 amino acids (aa) in length, and contains one kinase catalytic domain (aa 209‑522). There are two potential isoform variants that show either a six aa substitution for aa 1‑26, or this same substitution in combination with a premature truncation after Gln263.

  • Long Name:
    Dual-specificity Tyrosine-[Y]-phosphorylation Regulated Kinase 3
  • Entrez Gene IDs:
    8444 (Human); 226419 (Mouse); 304775 (Rat)
  • Alternate Names:
    dual specificity tyrosine-phosphorylation-regulated kinase 3; dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 3; dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 5; DYRK3; DYRK5; EC 2.7.12; EC 2.7.12.1; hYAK3-2; protein kinase Dyrk3; REDK; REDKRED; Regulatory erythroid kinase; YAK3-2
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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