Human/Mouse/Rat eIF5A Antibody

  
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human, mouse and rat eIF5A in  Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human eIF5A
    Lys85-Lys154
    Accession # P63241
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human, Mouse, and Rat eIF5A by Western Blot. Western blot shows lysates of 786‑O human renal cell adenocarcinoma cell line, C2C12 mouse myoblast cell line, and NR8383 rat alveolar macrophage cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat eIF5A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7558) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for eIF5A at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: eIF5A

eIF5A (Elongation initiation factor 5A; also eIF5A1, Rev-binding factor and eIF-4D) is a 16-19 kDa member of the eIF5A family of molecules. It is ubiquitously expressed, being found in all human cells examined. eIF5A acts as a stabilizer of RNA transcripts and acts as a positive modulator of p53-mediated apoptosis. It also may be involved in the formation of the first peptide bond during select protein synthesis, and is known to be essential for cell cycle progression through G1/S. The human eIF5A precursor is 154 amino acids (aa) in length. It contains a 71 aa DOHH (deoxyhypusine hydroxylase)-binding site (aa 20-90) and a 69 aa RNA-binding domain (aa 84-152). eIF5A undergoes a posttranslational modification that converts the precursor peptide into a mature molecule. This modification occurs on Lys50, and involves the transfer and hydroxylation of an aminobutyl sequence from spermidine, creating a unique amino acid termed hypusine (hydroxyputrisine lysine). When hypusine is unacetylated, eIF5A is active; when acetylated, eIF5A is inactive. Nonhypusinated eIF5A is found in both cytoplasm and nucleus, while hypusinated eIF5A is targeted to the cytoplasm. There is one isoform variant that shows a 32 aa substitution for aa 1-32. Over aa 85-154, human and mouse are identical in aa sequence. Over the same aa sequence, human eIF5A (on Chr 17p13) also shares 98% and 69% aa identity with human eIF5A1-Like (on Chr 10q22) and human eiF5A2 (on Chr 3q26), respectively.

  • Long Name:
    Eukaryotic Translation Initiation Factor 5A
  • Entrez Gene IDs:
    1984 (Human); 276770 (Mouse); 287444 (Rat)
  • Alternate Names:
    eIF4D; eIF-4D; eIF5A; EIF-5A; EIF5A1; eIF-5A1; eIF-5A-1; eIF5AI; Eukaryotic initiation factor 5A isoform 1; eukaryotic initiation factor 5A; eukaryotic translation initiation factor 5A; eukaryotic translation initiation factor 5A-1; MGC104255; MGC99547; Rev-binding Factor
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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