Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Simple Western

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Ezrin Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Ezrin
Lys438-Arg562
Accession # P15311

Specificity

Detects human, mouse, and rat Ezrin in Western blots and detects recombinant human Ezrin in direct ELISAs.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat Ezrin Antibody

Detection of Human, Mouse, and Rat Ezrin antibody by Western Blot.

Detection of Human, Mouse, and Rat Ezrin by Western Blot.

Western blot shows lysates of human placenta tissue, mouse placenta tissue, and C6 rat glioma cell line. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human/Mouse/Rat Ezrin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7239) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for Ezrin at approximately 81 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human Ezrin by Simple WesternTM.

Simple Western shows lysates of Exosome Standard (HEK293) (NBP3-11684), Exosome Standard (PC‑3) (NBP2-49856), and human cerebellum tissue, loaded at 0.5 mg/ml. A specific band was detected for Ezrin at approximately 76 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human/Mouse/Rat Ezrin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7239). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

Ezrin antibody in Human Colon Stem Cells by Immunohistochemistry (IHC-P).

Ezrin in Human Colon Stem Cells.

Ezrin was detected in immersion fixed paraffin-embedded sections of human colon stem cells differentiating into ascending colon using Sheep Anti-Human/Mouse/Rat Ezrin Antigen Affinity-purified Polyclonal Antibody (AF7239) at 0.5 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; NL010). Chromogranin A was also detected using Mouse Anti-Human Chromogranin A Monoclonal Antibody (MAB90981) at 0.5 µg/mL overnight at 4 °C. Tissue was co-stained using the NorthernLights™ 637-conjugated Anti-Mouse IgG Secondary Antibody (red; NL008) and counterstained with DAPI (blue). Specific staining of Ezrin was localized to microtubules. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Detection of Ezrin in Human Stomach

Ezrin was detected in immersion fixed paraffin-embedded sections of human stomach using Sheep Anti-Human/Mouse/Rat Ezrin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7239) at 1 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC006). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface of epithelial cells in gastric glands. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human/Mouse/Rat Ezrin Antibody

Application
Recommended Usage

Immunohistochemistry

0.5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human colon (colon stem cells differentiating into ascending colon) and human stomach

Simple Western

10 µg/mL
Sample: Exosome Standard (HEK293) (Catalog # NBP3-11684), Exosome Standard (PC-3) (Catalog # NBP2-49856), and human cerebellum tissue

Western Blot

0.2 µg/mL
Sample: Human placenta tissue, mouse placenta tissue, and C6 rat glioma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Ezrin

EZRIN (also Cytovillin, Villin2 and p81) is a founding member of the ERM family, Band 4.1 Superfamily of proteins. Although its predicted MW is 69 kDa, it runs anomalously at 77-82 kDa in SDS-PAGE. ERZIN is expressed by epithelial cells where it serves as a linker between the cell membrane and the actin cytoskeleton. Its presence is particularly strong in microvilli where it helps organize this structure. In addition, ERZIN also organizes microtubules in lymphocytes at or near the immunological synapse by interacting with Glg1. Human EZRIN is 585 amino acids (aa) in length. It contains a band 4.1 homology/FERM domain that binds CD44, ICAM-1, EBP50 and ERM family members (aa 1-295), a central alpha -helical region (aa 296-352), and a C-terminal ERM and actin-binding/FERM C domain (aa 353-586). EZRIN exists as either a monomer, or a homo/heterodimer. EZRIN is not constitutively active, but must be phosphorylated and unfolded to bind to cytoplasmic proteins. Over aa 438-562, human EZRIN shares 96% aa identity with mouse EZRIN.

Alternate Names

CVIL, CVL, Cytovillin 2, EZR, p81, VIL2, Villin 2

Entrez Gene IDs

7430 (Human); 22350 (Mouse); 54319 (Rat)

Gene Symbol

EZR

UniProt

P15311 (Human)

Additional Ezrin Products

Product Documents for Human/Mouse/Rat Ezrin Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat Ezrin Antibody

For research use only

Citations for Human/Mouse/Rat Ezrin Antibody

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Protocols

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