Human Chromogranin A Antibody Summary
Accession # P10645
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Chromogranin A in H69AR Human Cell Line. Chromogranin A was detected in immersion fixed H69AR human small cell lung cancer cell line using Mouse Anti-Human Chromogranin A Monoclonal Antibody (Catalog # MAB90981) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Chromogranin A in Human Parathyroid. Chromogranin A was detected in immersion fixed paraffin-embedded sections of human parathyroid using Mouse Anti-Human Chromogranin A Monoclonal Antibody (Catalog # MAB90981) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to principal cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Chromogranin A
Human Chromogranin A (CHGA) an acidic protein and a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is located in secretory vesicles of neurons and endocrine cells such as islet beta cell secretory granules in pancreas. Chromogranin A expression has been oberserved in neuroendocrine tumors, medullary thyroid carcinoma, as well as carcinoid tumors.
Citation for Human Chromogranin A Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Loss of MAGEL2 in Prader-Willi syndrome leads to decreased secretory granule and neuropeptide production
Authors: H Chen, AK Victor, J Klein, KF Tacer, DJ Tai, C de Esch, A Nuttle, J Temirov, LC Burnett, M Rosenbaum, Y Zhang, L Ding, JJ Moresco, JK Diedrich, JR Yates, HS Tillman, RL Leibel, ME Talkowski, DD Billadeau, LT Reiter, PR Potts
JCI Insight, 2020;5(17):.
Sample Types: Cell Lysates
Applications: Western Blot
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Reviews for Human Chromogranin A Antibody
Average Rating: 5 (Based on 1 Review)
Have you used Human Chromogranin A Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image