Human Chromogranin A Antibody

Catalog # Availability Size / Price Qty
MAB90981
MAB90981-SP
Chromogranin A in H69AR Human Cell Line.
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Product Details
Citations (1)
FAQs
Supplemental Products
Reviews (1)

Human Chromogranin A Antibody Summary

Species Reactivity
Human
Specificity
Detects human Chromogranin A in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 948128
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human embryonic kidney cell line HEK293-derived human Chromogranin A 
Met1-Gly457
Accession # P10645
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
5-25 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunocytochemistry Chromogranin A antibody in H69AR Human Cell Line by Immunocytochemistry (ICC). View Larger

Chromogranin A in H69AR Human Cell Line. Chromogranin A was detected in immersion fixed H69AR human small cell lung cancer cell line using Mouse Anti-Human Chromogranin A Monoclonal Antibody (Catalog # MAB90981) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry Chromogranin A antibody in Human Parathyroid by Immunohistochemistry (IHC-P). View Larger

Chromogranin A in Human Parathyroid. Chromogranin A was detected in immersion fixed paraffin-embedded sections of human parathyroid using Mouse Anti-Human Chromogranin A Monoclonal Antibody (Catalog # MAB90981) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to principal cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Chromogranin A

Human Chromogranin A (CHGA) an acidic protein and a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is located in secretory vesicles of neurons and endocrine cells such as islet beta cell secretory granules in pancreas. Chromogranin A expression has been oberserved in neuroendocrine tumors, medullary thyroid carcinoma, as well as carcinoid tumors.

Entrez Gene IDs
1113 (Human); 12652 (Mouse); 24258 (Rat)
Alternate Names
betagranin (N-terminal fragment of chromogranin A); Betagranin; Catestatin; CGA; CHGA; Chromofungin; chromogranin A (parathyroid secretory protein 1); Chromogranin A; chromogranin-A; Pancreastatin; Parastatin; Parathyroid Secretory Protein 1; Pituitary Secretory Protein I; SP-I

Product Datasheets

Citation for Human Chromogranin A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Loss of MAGEL2 in Prader-Willi syndrome leads to decreased secretory granule and neuropeptide production
    Authors: H Chen, AK Victor, J Klein, KF Tacer, DJ Tai, C de Esch, A Nuttle, J Temirov, LC Burnett, M Rosenbaum, Y Zhang, L Ding, JJ Moresco, JK Diedrich, JR Yates, HS Tillman, RL Leibel, ME Talkowski, DD Billadeau, LT Reiter, PR Potts
    JCI Insight, 2020;5(17):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Chromogranin A Antibody

Average Rating: 5 (Based on 1 Review)

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Human Chromogranin A Antibody
By Anonymous on 12/07/2021
Application: IHC Sample Tested: Prostatic adenocarcinoma tissue,Tumor tissue Species: Human