Detects human, mouse, and rat FABP3/H‑FABP in Western blots. In Western blots, approximately 15% cross-reactivity with recombinant rat (rr) FABP2 is observed and no cross-reactivity with rrFABP1, recombinant mouse (rm) FABP4, or rmFABP5 is observed.
Monoclonal Rat IgG2A Clone # 242305
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human FABP3/H‑FABP Met1-Ala133 Accession # P05413
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human, Mouse, and Rat FABP3/H‑FABP by Western Blot. Western blot shows lysates of human heart tissue, human skeletal muscle tissue, mouse heart tissue, and rat heart tissue. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Human/Mouse/Rat FABP3/H‑FABP Monoclonal Antibody (Catalog # MAB1678) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for FABP3/H‑FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
FABP3/H‑FABP in Human Heart. FABP3/H‑FABP was detected in immersion fixed paraffin-embedded sections of human heart using Rat Anti‑Human/Mouse/Rat FABP3/H‑FABP Monoclonal Antibody (Catalog # MAB1678) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human and Mouse FABP3/H‑FABP by Simple WesternTM. Simple Western lane view shows lysates of human heart tissue and mouse heart tissue, loaded at 0.2 mg/mL. A specific band was detected for FABP3/H‑FABP at approximately 19 kDa (as indicated) using 5 µg/mL of Rat Anti-Human FABP3/H‑FABP Monoclonal Antibody (Catalog # MAB1678) followed by 1:50 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Human FABP3, also known as FABP-Heart (H-FABP), FABP-Muscle (M-FABP) and mammary-derived growth inhibitor (MDGI), is a member of the intracellular FABP family. It plays a role in the transport of long chain fatty acids in the cytoplasm.
Fatty Acid-Binding Protein 3
Entrez Gene IDs:
FABP11; FABP3; fatty acid binding protein 11; fatty acid binding protein 3, muscle and heart (mammary-derived growthinhibitor); Fatty acid-binding protein 3; Fatty acid-binding protein 3, muscle; fatty acid-binding protein, heart; Heart-type fatty acid-binding protein; HFABP; H-FABP; H-FABPM-FABP; Mammary-derived growth inhibitor; MDGI; Muscle fatty acid-binding protein; O-FABP
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
Have you used Human/Mouse/Rat FABP3/H-FABP Antibody?
Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.