FABP3/H-FABP Antibody (242305)

R&D Systems | Catalog # MAB1678

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, Simple Western

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 242305
View all FABP3/H-FABP Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human FABP3/H‑FABP
Met1-Ala133
Accession # P05413

Specificity

Detects human, mouse, and rat FABP3/H‑FABP in Western blots. In Western blots, approximately 15% cross-reactivity with recombinant rat (rr) FABP2 is observed and no cross-reactivity with rrFABP1, recombinant mouse (rm) FABP4, or rmFABP5 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for FABP3/H-FABP Antibody (242305)

Detection of Human, Mouse, and Rat FABP3/H-FABP antibody by Western Blot.

Detection of Human, Mouse, and Rat FABP3/H‑FABP by Western Blot.

Western blot shows lysates of human heart tissue, human skeletal muscle tissue, mouse heart tissue, and rat heart tissue. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Human/Mouse/Rat FABP3/H-FABP Monoclonal Antibody (Catalog # MAB1678) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for FABP3/H-FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
FABP3/H-FABP antibody in Human Heart by Immunohistochemistry (IHC-P).

FABP3/H‑FABP in Human Heart.

FABP3/H-FABP was detected in immersion fixed paraffin-embedded sections of human heart using Rat Anti-Human/Mouse/Rat FABP3/H-FABP Monoclonal Antibody (Catalog # MAB1678) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counter-stained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human and Mouse FABP3/H-FABP antibody by Simple WesternTM.

Detection of Human and Mouse FABP3/H‑FABP by Simple WesternTM.

Simple Western lane view shows lysates of human heart tissue and mouse heart tissue, loaded at 0.2 mg/mL. A specific band was detected for FABP3/H-FABP at approximately 19 kDa (as indicated) using 5 µg/mL of Rat Anti-Human FABP3/H-FABP Monoclonal Antibody (Catalog # MAB1678) followed by 1:50 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for FABP3/H-FABP Antibody (242305)

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human heart

Simple Western

5 µg/mL
Sample: Human heart tissue and mouse heart tissue

Western Blot

0.5 µg/mL
Sample: Human heart tissue, human skeletal muscle tissue, mouse heart tissue, and rat heart tissue

Reviewed Applications

Read 1 review rated 5 using MAB1678 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FABP3/H-FABP

Human FABP3, also known as FABP-Heart (H-FABP), FABP-Muscle (M-FABP) and mammary-derived growth inhibitor (MDGI), is a member of the intracellular FABP family. It plays a role in the transport of long chain fatty acids in the cytoplasm.

Long Name

Fatty Acid-Binding Protein 3

Alternate Names

H-FABP, HFABP, MDGI

Entrez Gene IDs

2170 (Human)

Gene Symbol

FABP3

UniProt

P05413

Additional FABP3/H-FABP Products

Product Documents for FABP3/H-FABP Antibody (242305)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for FABP3/H-FABP Antibody (242305)

For research use only

Related Research Areas

Cardiac Stem Cell Markers
Obesity

Citations for FABP3/H-FABP Antibody (242305)

Customer Reviews for FABP3/H-FABP Antibody (242305) (1)

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Customer Images

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  • Human/Mouse/Rat FABP3/H-FABP Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Intestine
    Species: Mouse
    Verified Customer | Posted 10/14/2021
    Mouse FABP3/H-FABP Antibody, staining in intestine tissue.
    Fixed paraffin embedded sections. Samples were incubated with primary antibody for 13 hours, dilution 1:200.
    FABP3/H-FABP Antibody (242305) MAB1678

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Protocols

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