Human/Mouse/Rat FAK Antibody

Catalog # Availability Size / Price Qty
AF4467
AF4467-SP
Detection of Human, Mouse, and Rat FAK by Western Blot.
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Product Details
Citations (2)
FAQs
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Human/Mouse/Rat FAK Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse and rat FAK.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human FAK
Asp213-Thr412
Accession # Q05397
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Knockout Validated
FAK is specifically detected in HEK293T human embryonic kidney parental cell line but is not detectable in FAK knockout HEK293T cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human, Mouse, and Rat FAK antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat FAK by Western Blot. Western blot shows lysates of MCF‑7 human breast cancer cell line, HUVEC human umbilical vein endothelial cells, Neuro‑2A mouse neuroblastoma cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat FAK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4467) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for FAK at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Immunohistochemistry FAK antibody in Human Brain by Immunohistochemistry (IHC-P). View Larger

FAK in Human Brain. FAK was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using 3 µg/mL Human/Mouse/Rat FAK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4467) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Knockout Validated Western Blot Shows Human FAK Antibody Specificity by Using Knockout Cell Line. View Larger

Western Blot Shows Human FAK Specificity by Using Knockout Cell Line. Western blot shows lysates of HEK293T human embryonic kidney parental cell line and FAK knockout HEK293T cell line (KO). PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat FAK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4467) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FAK at approximately 135 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FAK

Focal adhesion kinase 1 (FAK) is a ubiquitously expressed non-receptor protein tyrosine kinase that is concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. This cellular localization is directed by a "Focal Adhesion Targeting" (FAT) sequence, a 125 amino acid sequence at the C-terminus. FAK plays an important role in migration, cell spreading, differentiation, cytoskeleton protein phosphorylation, apoptosis and acceleration of the G1 to S phase transition of the cell cycle. It associates with several different signaling proteins such as Src-family PTKs, p130Cas, Shc, Grb2, PI 3-kinase, and paxillin. This enables FAK to function within a network of integrin-stimulated signaling pathways leading to the activation of targets such as the ERK and JNK/mitogen-activated protein kinase pathways. FAK is also linked to oncogenes at biochemical and functional levels. Increased expression and/or activity of FAK in various tumors has been correlated with enhanced migration and invasiveness of human tumor cells in addition to promoting increased cell proliferation.

Long Name
Focal adhesion kinase 1
Entrez Gene IDs
5747 (Human); 14083 (Mouse); 25614 (Rat)
Alternate Names
EC 2.7.10; FADK 1; FADK1; FADKFAK-related non-kinase polypeptide; FAK; FAK1FRNK; FAKEC 2.7.10.2; focal adhesion kinase 1; pp125FAK; Protein-tyrosine kinase 2; PTK2 protein tyrosine kinase 2; PTK2

Product Datasheets

Citations for Human/Mouse/Rat FAK Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Focal adhesion kinase coordinates costamere-related JNK signaling with muscle fiber transformation after Achilles tenotomy and tendon reconstruction
    Authors: C Ferrié, S Kasper, F Wanivenhau, M Flück
    Exp. Mol. Pathol., 2019;0(0):.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: ELISA Development (Capture)
  2. Knee Extensors Muscle Plasticity Over a 5-Years Rehabilitation Process After Open Knee Surgery
    Authors: M Flück, C Viecelli, AM Bapst, S Kasper, P Valdivieso, MV Franchi, S Ruoss, JM Lüthi, M Bühler, H Claassen, H Hoppeler, C Gerber
    Front Physiol, 2018;9(0):1343.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Immunoprecipitation

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