Human/Mouse/Rat FKBP12.6 Antibody

  (2 citations)     
Datasheet / CoA / SDS
Product Details
Citations (2)
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  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human, mouse, and rat FKBP12.6 in Western blots. In Western blots, approximately 15% cross-reactivity with recombinant human FKBP12 and less than 1% cross-reactivity with recombinant human FKBP13 and FKBP25 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human FKBP12.6
    Met1-Glu108
    Accession # P68106
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Simple Western
    50 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human/Mouse/Rat FKBP12.6 by Western Blot. Western blot shows lysates of human cortex, human hippocampus, and mouse brain tissues. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat FKBP12.6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4174) followed by HRP‑conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for FKBP12.6 at approximately 13 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.
Immunohistochemistry
FKBP12.6 in Human Brain. FKBP12.6 was detected in immersion fixed paraffin-embedded sections of human brain (caudate nucleus) using Goat Anti-Human/Mouse/Rat FKBP12.6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4174) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of Human and Mouse FKBP12.6 by Simple WesternTM. Simple Western lane view shows lysates of human brain (cortex) tissue and mouse brain (cortex) tissue, loaded at 0.2 mg/mL. A specific band was detected for FKBP12.6 at approximately 16 kDa (as indicated) using 50 µg/mL of Goat Anti-Human/Mouse/Rat FKBP12.6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4174) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FKBP12.6
FK506 binding protein, 12.6 kDa molecular weight (FKBP12.6), also called FKBP1B, is a peptidyl-prolyl isomerase that catalyzes the transition between cis- and trans‑ proline residues critical for proper folding of proteins. The immunosuppressant FK506 is a potent inhibitor of FKBP12.6. FKBP12.6 is localized in the sarcoplasmic reticulum associated with the Ryanodyne receptor RYR2. PKA-induced phosphorylation of RYR2 causes FKBP12.6 dissociation. In heart failure, RYR2 is hyperphosphorylated and has low levels of FKBP12.6 bound to it. Male FKBP12.6 knockout mice have cardiac hypertrophy and lethal exercise arrhythmias.
  • Long Name:
    12.6 kDa FK506 Binding Protein
  • Entrez Gene IDs:
    2281 (Human)
  • Alternate Names:
    12.6 kDa FK506-binding protein; calstabin 2,12.6 kDa FKBP; EC 5.2.1.8; FK506 binding protein 1B, 12.6 kDa; FK506-binding protein 12.6; FK506-binding protein 1B (12.6 kD); FK506-binding protein 1B; FKBP12.6; FKBP-12.6; FKBP12.6h-FKBP-12; FKBP1B; FKBP-1B; FKBP1L; FKBP9; Immunophilin FKBP12.6; OTK4; OTK4PPIase FKBP1B; peptidyl-prolyl cis-trans isomerase FKBP1B; PKBP1L; PPIase; rotamase
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. Impact of hypoxia, simulated ischemia and reperfusion in HL-1 cells on the expression of FKBP12/FKBP12.6 and intracellular calcium dynamics.
    Biochem. Biophys. Res. Commun., 2102;422(4):732-8.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  2. Specific and Novel microRNAs Are Regulated as Response to Fungal Infection in Human Dendritic Cells
    Authors: A Dix, K Czakai, I Leonhardt, K Schäferhof, M Bonin, R Guthke, H Einsele, O Kurzai, J Löffler, J Linde
    Front Microbiol, 2017;8(0):270.
    Species: Human
    Sample Type: Whole Cells
    Application: WB

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