Human/Mouse/Rat FKBP12.6 Antibody

Catalog # Availability Size / Price Qty
AF4174
AF4174-SP
Detection of Human/Mouse/Rat FKBP12.6 by Western Blot.
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Product Details
Citations (3)
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Human/Mouse/Rat FKBP12.6 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat FKBP12.6 in Western blots. In Western blots, approximately 15% cross-reactivity with recombinant human FKBP12 and less than 1% cross-reactivity with recombinant human FKBP13 and FKBP25 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human FKBP12.6
Met1-Glu108
Accession # P68106
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
50 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human/Mouse/Rat FKBP12.6 antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat FKBP12.6 by Western Blot. Western blot shows lysates of human cortex, human hippocampus, and mouse brain tissues. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat FKBP12.6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4174) followed by HRP‑conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for FKBP12.6 at approximately 13 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.

Western Blot Specificity of FKBP12.6 Shown by Western Blot. View Larger

Specificity of FKBP12.6 Shown by Western Blot. Western blot shows recombinant human FKBP 12, recombinant human FKBP 12.6, recombinant human FKBP 13, recombinant human FKBP 25, recombinant human FKBP 38, and recombinant human FKBP 52. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat FKBP12.6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4174) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for FKBP12.6 at approximately 13 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry FKBP12 by Immunohistochemistry (IHC-P).6 antibody in Human Brain by Immunohistochemistry (IHC-P). View Larger

FKBP12.6 in Human Brain. FKBP12.6 was detected in immersion fixed paraffin-embedded sections of human brain (caudate nucleus) using Goat Anti-Human/Mouse/Rat FKBP12.6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4174) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Simple Western Detection of Human and Mouse FKBP12.6 antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human and Mouse FKBP12.6 by Simple WesternTM. Simple Western lane view shows lysates of human brain (cortex) tissue and mouse brain (cortex) tissue, loaded at 0.2 mg/mL. A specific band was detected for FKBP12.6 at approximately 16 kDa (as indicated) using 50 µg/mL of Goat Anti-Human/Mouse/Rat FKBP12.6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4174) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FKBP12.6

FK506 binding protein, 12.6 kDa molecular weight (FKBP12.6), also called FKBP1B, is a peptidyl-prolyl isomerase that catalyzes the transition between cis- and trans‑ proline residues critical for proper folding of proteins. The immunosuppressant FK506 is a potent inhibitor of FKBP12.6. FKBP12.6 is localized in the sarcoplasmic reticulum associated with the Ryanodyne receptor RYR2. PKA-induced phosphorylation of RYR2 causes FKBP12.6 dissociation. In heart failure, RYR2 is hyperphosphorylated and has low levels of FKBP12.6 bound to it. Male FKBP12.6 knockout mice have cardiac hypertrophy and lethal exercise arrhythmias.

Long Name
12.6 kDa FK506 Binding Protein
Entrez Gene IDs
2281 (Human)
Alternate Names
12.6 kDa FK506-binding protein; calstabin 2,12.6 kDa FKBP; EC 5.2.1.8; FK506 binding protein 1B, 12.6 kDa; FK506-binding protein 12.6; FK506-binding protein 1B (12.6 kD); FK506-binding protein 1B; FKBP12.6; FKBP-12.6; FKBP12.6h-FKBP-12; FKBP1B; FKBP-1B; FKBP1L; FKBP9; Immunophilin FKBP12.6; OTK4; OTK4PPIase FKBP1B; peptidyl-prolyl cis-trans isomerase FKBP1B; PKBP1L; PPIase; rotamase

Product Datasheets

Citations for Human/Mouse/Rat FKBP12.6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Impact of hypoxia, simulated ischemia and reperfusion in HL-1 cells on the expression of FKBP12/FKBP12.6 and intracellular calcium dynamics.
    Biochem. Biophys. Res. Commun., 2102;422(4):732-8.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Altered calcium-handling produces reentry-promoting action potential alternans in atrial fibrillation-remodeled hearts
    Authors: T Liu, F Xiong, XY Qi, J Xiao, L Villeneuve, I Abu-Taha, D Dobrev, C Huang, S Nattel
    JCI Insight, 2020;0(0):.
    Species: Canine
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Specific and Novel microRNAs Are Regulated as Response to Fungal Infection in Human Dendritic Cells
    Authors: A Dix, K Czakai, I Leonhardt, K Schäferhof, M Bonin, R Guthke, H Einsele, O Kurzai, J Löffler, J Linde
    Front Microbiol, 2017;8(0):270.
    Species: Human
    Sample Types: Whole Cells
    Applications: Western Blot

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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