Human/Mouse/Rat GABAB R1 Antibody

  
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects recombinant mouse and rat GABAB R1 in direct ELISAs and Western blots.Detects human and rat GABAB R1 in Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant rat GABAB R2 is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant rat GABAB R1
    Gly17-Leu586
    Accession # Q9Z0U4
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human and Rat GABAB R1 by Western Blot. Western blot shows lysates of IMR‑32 human neuroblastoma cell line and rat embryonic hippocampal neurons. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse/Rat GABAB R1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7000) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for GABAB R1 at approximately 70-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
GABAB R1 in Rat Brain. GABAB R1 was detected in perfusion fixed frozen sections of rat brain (dorsal root ganglia) using Sheep Anti-Mouse/Rat GABAB R1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7000) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Northern­Lights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to the cell bodies of dorsal root ganglia neurons. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GABA-B R1

GABAB R1 (GABA-B receptor subunit 1; also GABA-BR1, GABBR1 and GB1) is a multispan member of the GABA-B receptor subfamily, GPCR-3 family of proteins. It forms an obligatory heterodimer with GABA-BR2, creating a G-protein metabotropic GABA receptor that inhibits adenylyl cyclase activity and activates K+ channels. Presynaptically, this blocks neurotransmitter release; postsynaptically, it lowers neuron excitability. Rat GABAB R1 is 991 amino acids (aa) in length. It is a 7‑transmembrane glycoprotein that contains a 16 aa signal sequence, an extended N‑terminal extracellular region (aa 17-590) that contains two SUSHI domains (aa 29‑158), and a long C-terminal cytoplasmic domain (aa 885-991). There are several splice variants with predicted molecular weights ranging from 90 to 111 kDa and multiple glycosylation sites. The 991 aa isoform described above is called GABAB R1e (R1e). There is also a 960 aa, 130 kDa isoform that shows a deletion of aa 771‑801. This variant (R1a) is associated with postsynaptic membranes. A third isoform (R1b) is 844 aa in length and 100 kDa in size, and possesses both a deletion of aa 771-801, and a 47 aa substitution for aa 1-163. This variant is presynaptic in location. Two other isoforms are variants of GABAB R1b. Each show the same N‑terminal substitution, with a fourth isoform (R1c) retaining aa 771-801, and a fifth isoform (R1d) deleting aa 771-801, coupled to a 25 aa substitution for aa 935‑991. Over aa 17-586, rat GABAB R1e/a shares 99% aa identity with both mouse and human GABAB R1.

  • Long Name:
    gamma-Aminobutyric Acid Type B Receptor Subunit 1
  • Entrez Gene IDs:
    2550 (Human); 543931 (Mouse); 81657 (Rat)
  • Alternate Names:
    FLJ92613; GABA B R1; GABABR1; GABBR1; GABBR1-3; gamma-aminobutyric acid (GABA) B receptor, 1; gamma-aminobutyric acid type B receptor subunit 1; Gb1; GPRC3A; GPRC3Asubunit 1c; hGB1a; seven transmembrane helix receptor
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