Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Western Blot, Simple Western

Cited:

Immunohistochemistry, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all GRP78/HSPA5 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human GRP78/HSPA5
Met1-Leu654
Accession # P11021

Specificity

Detects endogenous human, mouse and rat GRP78 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat GRP78/HSPA5 Antibody

Detection of Human and Rat GRP78/HSPA5 antibody by Western Blot.

Detection of Human and Rat GRP78/HSPA5 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and A20 mouse B cell lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat GRP78/HSPA5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4846) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for GRP78/HSPA5 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Detection of Human GRP78/HSPA5 antibody by Simple WesternTM.

Detection of Human GRP78/HSPA5 by Simple WesternTM.

Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for GRP78/HSPA5 at approximately 74 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat GRP78/HSPA5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4846) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human/Mouse/Rat GRP78/HSPA5 Antibody

Application
Recommended Usage

Simple Western

5 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line

Western Blot

0.5 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, A20 mouse B cell lymphoma cell line, and rat colon tissue

Reviewed Applications

Read 1 review rated 5 using AF4846 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GRP78/HSPA5

GRP78 (Glucose-regulated protein 78 kDa; also BiP and HSPA5) is a 72 kDa member of the heat shock protein 70 family of proteins. Intracellularly, GRP78 is an endoplasmic reticulum chaperone that participates in protein folding; extracellularly, it induces IL-10 production from T cells and interacts with Cripto to block TGF-beta signaling. Human GRP78 precursor is 654 amino acids (aa) in length. It contains an 18 aa signal sequence and a 636 aa mature region that shows a hydantoinase A region (aa 145‑245) and a C-terminal KDEL motif that is present on intracellular GRP78, but absent on secreted GRP78. There is alternative splicing in the signal sequence (aa 1‑10), and multiple single aa substitituion. Over aa 1‑654, human GRP78 is more than 97% aa identical to mouse and rat GRP78.

Long Name

75 KD Glucose Regulated Protein

Alternate Names

BIP, HSP70-5, HSPA5

Entrez Gene IDs

3309 (Human); 14828 (Mouse); 25617 (Rat)

Gene Symbol

HSPA5

UniProt

P11021 (Human)

Additional GRP78/HSPA5 Products

Product Documents for Human/Mouse/Rat GRP78/HSPA5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat GRP78/HSPA5 Antibody

For research use only

Citations for Human/Mouse/Rat GRP78/HSPA5 Antibody

Customer Reviews for Human/Mouse/Rat GRP78/HSPA5 Antibody (1)

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  • Human/Mouse/Rat GRP78/HSPA5 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: A549 human lung carcinoma cell line and H69AR human small cell lung cancer cell line
    Species: Human
    Verified Customer | Posted 10/17/2018
    Human/Mouse/Rat GRP78/HSPA5 Antibody AF4846

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